We narrowed to 4,455 results for: TIL
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Plasmid#165920PurposeBlasticidin resistant 5xQUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable QUAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-12xLexAOp-sfGFP-MiniWhite
Plasmid#165916PurposeBlasticidin resistant 12xLexAOp sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable LexAOp Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xQUAS-mCherry-MiniWhite
Plasmid#165923PurposeBlasticidin resistant 5xQUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable QUAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc C1A4E
Plasmid#141088Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of NanolucDepositorInsertAntares-T2A-puromycin-IRESv24-SEP-C1A4E
UseTagsExpressionMammalianMutationPlease see depositor commentsPromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
D19_Ld_130940
Plasmid#135835PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertsubtilisin-like serine peptidase,serine peptidase, clan SB, family S8-like protein
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas9FnCpf1GG
Plasmid#131013PurposeBackbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.DepositorInsertsdirect repeat of SpCas9
GFP expression cassette
direct repeat of FnCas12a
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a(cd96)
Plasmid#177901PurposeBacterial expression of his10-tagged human cd96 Intracellular DomainDepositorInsertCD96 (aa 557-585 only) (CD96 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
HMGA2(3'UTR DEL)+GFP
Plasmid#25406DepositorInserthigh mobility group AT-Hook 2 (Hmga2 Mouse)
UseLentiviralTagsExpressionMammalianMutation3" UTR is absentPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV/D377Y-mPCSK9
Plasmid#58376PurposeExpresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosisDepositorUseAAVTagsExpressionMammalianMutationD377YPromoterHCRApoE/hAATAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
NowGFP /pQE-30
Plasmid#74749PurposeNowGFP gene for bacterial expressionDepositorInsertNowGFP
UseTagsHisTag (with a mutation but still working)ExpressionBacterialMutationWasCFP with substitutions E6K, L42M, T43S, V68M, …PromoterT5Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Puro-P2A-EGFP
Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-SorLA-v1
Plasmid#154892PurposeExpresses SorLA fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assaysDepositorInsertSORL1 (SORL1 Human)
UseTagsVenus fragment 1 (v1)ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV/D374Y-hPCSK9
Plasmid#58379PurposeExpresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosisDepositorInsertproprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
UseAAVTagsExpressionMammalianMutationD374YPromoterHCRApoE/hAATAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST-SorLA-v2
Plasmid#154900PurposeExpresses SorLA fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assaysDepositorInsertSORL1 (SORL1 Human)
UseTagsVenus fragment 2 (v2)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-aBEST
Plasmid#131464PurposeExpression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetesDepositorInsertsStreptomyces codon optimized spCas9n (D10A)
streptomyces codon optimized tadA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromotertipAAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-GST-4EBP1-Low GC
Plasmid#69018Purposeexpresses tagged 4EBP1DepositorInsertEIF4EBP1 (EIF4EBP1 Human)
UseTagsGST and HAExpressionMammalianMutationlowGC content, wt is ~70% GC rich. Still, it code…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-mcBEST
Plasmid#209416PurposePlasmid for multiplexed cytosine base editing in streptomycetesDepositorInsertsCodon optimized APOBEC1-nCas9-UGI fusion protein
csy4 from Pseudomonas aeruginosa PAO1
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterbase editing cassette: PtipA ; sgRNAs: PkasO* an…Available sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-IFNA-neo
Plasmid#225710PurposeThe pCDH-IFNA-neo plasmid is designed for the overexpression of human IFNα in mammalian cells. This plasmid was utilized to engineer A549 cells for stable IFNα overexpression.DepositorInsertIFNA (IFNA1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
SiriusGFP
Plasmid#123201PurposeWe have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.DepositorInsertSiriusGFP
UseMouse TargetingTagsN/AExpressionBacterial and MammalianMutationeGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…PromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEA01
Plasmid#170766PurposeUtilisation of pSAM2 excision tool in Amycolatopsis sppDepositorInsertsxis, int
oriT
pac
UseTagsExpressionBacterialMutationPromotertrcpAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only