We narrowed to 10,059 results for: Uty

1. pSEMS-SDHB-Halo7Tag

   Plasmid

   #108973

   Purpose

   Selflabeling tag at succinate dehydrogenase complex subunit B (SDHB)

   Depositor

   Karin Busch

   Article

   Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

   Insert

   succinate dehydrogenase complex iron sulfur subunit B (SDHB Human)
   

   Tags

   Halo7-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLCHKO

   Plasmid

   #155048

   Purpose

   Lentiviral backbone for cloning and expressing U6 driven hybrid guide (hg)RNAs with BfuAI cloning sites and puromycin selection.

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFPC1 MOSPD1

   Plasmid

   #226404

   Purpose

   Expression of human MOSPD1 fused to EGFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

   Insert

   MOSPD1 (MOSPD1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMCL-HA-MAPKK1-G1C [delta(44-51)/S218E/S222D]

   Plasmid

   #40807

   Depositor

   Natalie Ahn

   Article

   Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta(44-51), S218E, S222D

   Promoter

   CMV

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

5. p35u4

   Plasmid

   #174786

   Purpose

   Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system

   Depositor

   Katherine Berry

   Article

   Wang et al RNA. 2021 Apr;27(4):513-526. doi: 10.126

   Insert

   MS2 coat protein
   

   Tags

   λCI

   Expression

   Bacterial

   Mutation

   V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…

   Promoter

   Synthetic Constitutive Promoter

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNAIIIB cdc42 QL

   Plasmid

   #61636

   Purpose

   mammalian expression of cdc42 constitutively active mutant

   Depositor

   JS Gutkind

   Article

   Coso et al Cell. 1995 Jun 30;81(7):1137-46.

   Insert

   Cdc42 (CDC42 Human)
   

   Expression

   Mammalian

   Mutation

   Q61L, constitutively active

   Promoter

   CMV

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCDNAIIIB rhoA QL

   Plasmid

   #61632

   Purpose

   mammalian expression of rhoA constitutively active mutant

   Depositor

   JS Gutkind

   Article

   Coso et al Cell. 1995 Jun 30;81(7):1137-46.

   Insert

   rhoA (RHOA Human)
   

   Expression

   Mammalian

   Mutation

   Q63L, constitutively active

   Promoter

   CMV

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

8. SaCas9 TadCBEa

   Plasmid

   #193844

   Purpose

   Express TadCBEa (with SaCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   SaCas9 TadCBEa
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHM123DEnv

   Plasmid

   #137668

   Purpose

   Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

   Depositor

   Anjali Joshi

   Article

   Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

   Insert

   HIV-1 Envelope Glycoprotein
   

   Expression

   Mammalian

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEDF-PhdRS

    Plasmid

    #127445

    Purpose

    Double mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophage

    Depositor

    Wenshe Liu

    Article

    Tharp et al Nat Commun. 2020 Mar 13;11(1):1392. doi:

    Insert

    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    

    Expression

    Bacterial

    Mutation

    N346A-C348A mutations on PylRS

    Promoter

    araBAD

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Dendra2-Vinculin-N-21

    Plasmid

    #57749

    Purpose

    Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    NM_003373.3 (VCL Human)
    

    Tags

    Dendra2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pETM6-RgTALsyn-HpaBC

    Plasmid

    #100949

    Purpose

    Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Inserts

    RgTALsyn
    HpaB
    HpaC
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. WTTEM1_pSTC0-zeo

    Plasmid

    #202351

    Purpose

    Control WT TEM-1 (wild type TEM-1 beta lactamase) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    TEM-1
    

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP

    Plasmid

    #192273

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloning

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-mTDGa.0

    Plasmid

    #81050

    Purpose

    bacterial expression of murine TDG

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TDG (Tdg Mouse)
    

    Tags

    6HIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W

    Plasmid

    #105034

    Purpose

    Lentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Ptpn23 (Ptpn23 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)

    Plasmid

    #191647

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletion

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABE FLAG-IKK2-EE puro

    Plasmid

    #216790

    Purpose

    Mammalian expression of constitutively active human IKK-2

    Depositor

    Kevin Janes

    Article

    Griffiths et al Sci Adv. 2024 Nov 15;10(46):eadp6303. do

    Insert

    IKK-2 (IKBKB Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S177E and S181E, constitutively active

    Promoter

    LTR

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

    Plasmid

    #105039

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX

    Plasmid

    #188992

    Purpose

    A plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    K109R, P245A, G328S, G381V, G429D

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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