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pET21-pelB-GST-1

Plasmid

#228983

Purpose

For bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-1

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-3

Plasmid

#228985

Purpose

For bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-3

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-1

Plasmid

#228974

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-1

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCHW111118

Plasmid

#222461

Purpose

Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-CMV1 (Cucumber Mosaic Virus)

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW111101

Plasmid

#222452

Purpose

Level 1, Position 7 containing CBS4-miniDFR::Cre::NOS.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CBS4-miniDFR::Cre::NOS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-ALFA-CD28

Plasmid

#223630

Purpose

Lentiviral expression of human CD28 with ALFA tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

ALFA tag

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-mGFP-Eps15DN

Plasmid

#223621

Purpose

Transient expression of human Eps15 with mGFP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

Eps15 (EPS15 Human)

Tags

mGFP

Expression

Mammalian

Mutation

Eps15 _ 95-295aa

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SmBiT-CD80

Plasmid

#223614

Purpose

Lentiviral expression of human CD80 with SmBiT tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

SmBiT

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD86-mTFP1

Plasmid

#223606

Purpose

Lentiviral expression of human CD86 with mTFP1 tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

mTFP1

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-CD86iso1

Plasmid

#223601

Purpose

Lentiviral expression of human CD86 isoform 1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 isotype1 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCDF-rM1PYK

Plasmid

#220232

Purpose

Inducible expression of rabbit muscle pyruvate kinase isoform 1

Depositor

Aron Fenton, Liskin Swint-Kruse

Article

Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

Insert

Rabbit muscle pyruvate kinase (PKM )

Tags

6xHis-MBP-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-CRT-Fc FL

Plasmid

#221853

Purpose

Express human Calreticulin (full-length) with C-terminal human IgG1Fc tag in mammalian cell.

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

calreticulin (CALR Human)

Tags

Human IgG1Fc tag

Expression

Mammalian

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pENN491_TALEcharm_MmT8x

Plasmid

#220836

Purpose

TALEcharm Kv2 expression in mammalian cells; targets Prnp

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

TALEcharm

Use

Lentiviral and TALEN

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

EF1a

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCDF-hLPYK

Plasmid

#220231

Purpose

Inducible expression of human liver pyruvate kinase

Depositor

Aron Fenton, Liskin Swint-Kruse

Article

Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

Insert

Human liver pyruvate kinase (PKLR Human)

Expression

Bacterial

Promoter

T7

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2

Plasmid

#210019

Purpose

tetO-OKS (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + Sox2)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Oct4-p2a-Klf4-IRES-Sox2 (Klf4, Pou5f1, Sox2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO-mini-CMV

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgBAK-2

Plasmid

#210132

Purpose

knock out BAK in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Bcl-2 homologous antagonist/killer (BAK1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgCYFIP1-2

Plasmid

#210138

Purpose

knock out CYFIP1 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Cytoplasmic FMR1-interacting protein 1 (CYFIP1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_12xTEAD_luc2

Plasmid

#173004

Purpose

firefly luciferase reporter vector with 12x clustered TEAD binding sites

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

12xTEAD-luc2

Use

AAV and Luciferase

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-ACSL4-FL

Plasmid

#202515

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -1 to -2844 with respect to exon1 start site)

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Pcdh15_mini_V7-CD2

Plasmid

#199186

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

mmPcdh15_mini_V7-CD2

Use

AAV

Expression

Mammalian

Mutation

EC4, EC5, EC6, EC9, EC10 are deleted from msPCDH1…

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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