We narrowed to 6,132 results for: cat.2

1. psiCHECK2-mMOV10-3'UTR-MRE16+MRE153-mut

   Plasmid

   #178910

   Purpose

   dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   3'UTR mMov10 (Mov10 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   CTG>AGT and TGC>CAT mir16+153 mutated

   Promoter

   SV40 promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-KrasWT/sgKras/Cre

   Plasmid

   #99848

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Promoter

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #223321

   Purpose

   It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.

   Depositor

   Jonathan Arias

   Article

   Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

   Inserts

   SpCas9 codon optimized for human (cas9 Synthetic, Human)
   chloramphenicol resistance protein
   CcdB
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

   Plasmid

   #227610

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Use

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 2-400, 706-757 [TM and HB2 deleted]

   Promoter

   AOX1

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPA101

   Plasmid

   #17256

   Depositor

   Nancy Craig

   Article

   Gamas et al Nucleic Acids Res. 1992 May 25. 20(10):2

   Insert

   TnsC (tnsC E. coli Tn7)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAMPK alpha2 delta312X

   Plasmid

   #60127

   Purpose

   expresses constitutively active AMPKalpha2

   Depositor

   Morris Birnbaum

   Insert

   AMPK alpha 2 (Prkaa2 Rat)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Truncated at residue 312

   Promoter

   Available since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1 puro shTEAD1/3/4-1

   Plasmid

   #193672

   Purpose

   Constitutive lentiviral expression of TEAD shRNA

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   TEAD
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1 puro shPRDM14-1

   Plasmid

   #193694

   Purpose

   Constitutive lentiviral expression of PRDM14 shRNA

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   PRDM14 (PRDM14 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1 puro shPRDM14-5

   Plasmid

   #193698

   Purpose

   Constitutive lentiviral expression of PRDM14 shRNA

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   PRDM14 (PRDM14 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBolux

    Plasmid

    #188109

    Purpose

    Encodes a promoter-less Bacteroides-optimized lux cassette

    Depositor

    Guy Townsend

    Article

    Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Akaby

    Bacterial Strain

    #184224

    Purpose

    Akaby is a RecB knockout E. coli strain, made for cell-free expression system for linear templates.

    Depositor

    Kate Adamala

    Article

    Sato et al PLoS One. 2022 Apr 7;17(4):e0266272. doi

    Bacterial Resistance

    Kanamycin

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

12. HOXC5 (human) HIS-tag pET

    Plasmid

    #8563

    Depositor

    Corey Largman

    Insert

    HOXC5 (HOXC5 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Starts at Amino Acid #2 (Ser); makes protein by W…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pPPI4-Strep-SNAP-PD-1

    Plasmid

    #223569

    Purpose

    Protein purification of human PD-1 with Twin-Strep and SNAP tags from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

    Insert

    PD-1 (PDCD1 Human)
    

    Use

    Tags

    Twin-Strep, SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC001 - huLshC2C2-MBP for bacterial expression

    Plasmid

    #79150

    Purpose

    Expresses human codon-optimized LshC2c2 for purification in E. coli

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    C2c2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cas12f-GE ver4.0

    Plasmid

    #176543

    Purpose

    Expresses Cas12f-GE in mammalian cells

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

    Inserts

    Cas12f-GE ver4.0
    Cas12f-GE ver4.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken β-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cas12f-GE ver3.0

    Plasmid

    #176508

    Purpose

    Expresses Cas12f-GE in mammalian cells

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

    Inserts

    Cas12f-GE ver3.0
    Cas12f-GE ver3.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken β-actin

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CAG-eYFP-3x-miR204-5p-TS

    Plasmid

    #117380

    Purpose

    An AAV genome containing miRNA target sequence (TS) 204-5p to reduce expression of the fluorescent protein eYFP in astrocytes

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSI2-ADA

    Plasmid

    #166967

    Purpose

    Express the fusion protein of human MSI2 and the hyperactive catalytic domain of Drosophila ADAR

    Depositor

    Michael Kharas

    Article

    Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

    Insert

    MSI2-ADA
    

    Use

    Retroviral

    Tags

    MSI2-ADA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUCmini-iCAP-PHP.B

    Plasmid

    #103002

    Purpose

    non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cas12f-GE ver4.1

    Plasmid

    #176544

    Purpose

    Expresses Cas12f-GE in mammalian cells

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

    Inserts

    Cas12f-GE ver4.1
    Cas12f-GE ver4.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken β-actin

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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