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nSpG UGI Puro

Plasmid

#235046

Purpose

Control nickase Cas9

Depositor

Justin Pritchard

Article

Sokirniy et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

nCas9

Use

CRISPR and Lentiviral

Tags

P2A-PuroR

Promoter

EF1a core

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL814

Plasmid

#204133

Purpose

Bacillus cereus (B. ce) fluoride riboswitch, pseudoknot/terminator overlap from T. pe

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

B. ce fluoride riboswitch with T. pe pseuoknot regulated sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL815

Plasmid

#204134

Purpose

Thermotoga petrophila (T. pe) fluoride riboswitch, pseudoknot/terminator overlap from B. ce

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

T. pe fluoride riboswitch with B. ce pseuoknot regulated sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL831

Plasmid

#204137

Purpose

Thermotoga petrophila (T. pe) fluoride riboswitch, W1D4

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

T. pe fluoride riboswitch with wobble at position one and decreasing mutant at position 4 regulated sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-NanA-CSS-ET64

Plasmid

#234666

Purpose

Expression an ELP-tagged fusion enzyme of NanA and CSS

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

sialic acid aldolase
CMP-sialic acid synthetase

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-ManC-NahK-ET64

Plasmid

#234664

Purpose

Expression an ELP-tagged fusion enzyme of PfManC and NahK

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

GDP-Man pyrophosphorylase
N-acetylhexosamine 1-kinase

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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esgRNA_NbmiR164e/h

Plasmid

#231157

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting NbmiR164e/h

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNA targeting NbmiR164e/h

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-larE-larC

Plasmid

#232793

Purpose

Expression of L. plantarum larE and LarC genes in E. coli

Depositor

Robert Hausinger

Article

Nevarez et al mBio. 2024 Dec 16:e0340424. doi: 10.1128

Inserts

Pyridinium-3,5-bisthiocarboxylic acid mononucleotide synthase
Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein

Expression

Bacterial

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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esgRNA_SlMIR164b

Plasmid

#231153

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlMIR164b

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNA targeting SlMIR164b

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pDY1935

Plasmid

#212315

Purpose

Express ApmFnuc protein with sumotag

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

Isvmimi

Tags

6xHis-thrombin site-TwinStrepTag-SUMO

Expression

Bacterial

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGL2084

Plasmid

#137726

Purpose

Gateway vector for inducible stem loop RNAi in T.b. brucei 2T1

Depositor

Jeremy Mottram

Article

Jones et al PLoS Pathog. 2014 Jan;10(1):e1003886. do

Type

Empty backbone

Use

RNAi; Trypanosoma brucei tet inducible stem loop

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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SaCas9-mSA

Plasmid

#182953

Purpose

Express SaCas9-mSA

Depositor

Wen Xue

Article

Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

Insert

SaCas9-mSA

Use

CRISPR

Expression

Mammalian

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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p8116 LentiCRISPR v2 sgPTPN14-4

Plasmid

#163316

Purpose

Expresses Cas9 and a human PTPN14-targeting control guide RNA

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

sgPTPN14-4

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-FFSARG-ddFLN4-HIS-ybbr

Plasmid

#101240

Purpose

minimal SdrG binding peptide FFSARG with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

Depositor

Hermann Gaub

Article

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

FFSARG peptide, ddFLN4 fingerprint

Tags

HIS and ybbr

Expression

Bacterial

Mutation

C18S in ddFLN4

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJB172

Plasmid

#86992

Purpose

CRISPR/Cas9 in fission yeast using fluoride selection and targetting pil1

Depositor

Julien Berro

Article

Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

Insert

gRNA targeting pil1

Expression

Yeast

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pJK130

Plasmid

#71129

Purpose

Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide synthetase

Tags

His6

Expression

Bacterial

Mutation

changed valine-319 to phenylalanine

Promoter

T7

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pJK173

Plasmid

#71831

Purpose

Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE, and purK genes

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Inserts

hypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase

Expression

Bacterial

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pJK174

Plasmid

#71835

Purpose

Expression of Acetobacter aceti 1023 PurE and PurK in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Inserts

N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase

Expression

Bacterial

Mutation

additional N-terminal methionine encoded by this …

Promoter

T7 and endogenous

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJK175

Plasmid

#71132

Purpose

Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

additional N-terminal methionine encoded by this …

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-RTRG

Plasmid

#69845

Purpose

The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

Depositor

Daniel Christophe

Article

Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

Insert

1 RTRG repeat

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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