We narrowed to 60,212 results for: LAS

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FFiBlast p5

Plasmid

#68470

Purpose

Inverted and floxed blasticidin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Blasticidin Resistance

Use

Cre/Lox

Expression

Mammalian

Mutation

Inverted

Promoter

LTR

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-dCas9-p300_Blast

Plasmid

#192653

Purpose

3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

dCas9-p300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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BlastR-P2A-DD-TCOF1-donor

Plasmid

#60748

Purpose

Donor plasmid for HR-mediated recombination to insert blasticidin resistance and append a destabilization domain to the N-terminus of TCOF1

Depositor

Benhur Lee

Article

Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

Insert

TCOF1 (TCOF1 Human)

Use

Targeting donor

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)

Plasmid

#114078

Purpose

CAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain

Tags

SV40 NLS

Expression

Mammalian

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS

Plasmid

#63170

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-OLLAS"

Tags

N-myristoylation

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-mScarlet

Plasmid

#207678

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mScarlet cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MYC-PGK-blast

Plasmid

#190618

Purpose

Lentiviral expression plasmid of human MYC, blast selection

Depositor

Junwei Shi

Article

Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-EA-BE4max-NG-BlastR

Plasmid

#152992

Purpose

High purity C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

BE4 H47E+S48A-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-eA3Amax-BlastR

Plasmid

#152997

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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Endoplasmic reticulum-targeted optoPLD

Plasmid

#140060

Purpose

Encodes CRY2-mCherry-PLD-P2A-CIBN-Sac1(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CIBN, CRY2, P2A, Sac1(TM), and mCherry

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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LB901: pMVP/PB/Blast-DEST

Plasmid

#121873

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCLIPf-Cox8A Control Plasmid

Plasmid

#101130

Purpose

Expression of CLIP-Tagged Cox8A in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Cox8A (COX8A Human)

Tags

CLIP-tag (CLIPf)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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LVXN-Neo-NSD2-E1099K

Plasmid

#86011

Purpose

express NSD2 E1099K mutant in mammalian cells

Depositor

Darrin Stuart

Article

Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

Insert

NSD2 E1099K mutant (NSD2 Human)

Tags

Flag tag D Y K D D D D K

Expression

Mammalian

Mutation

E1099K; K1002R (please see depositor comments bel…

Promoter

CMV

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only
pLV-Blast_hCOL4A5

Plasmid

#198100

Purpose

lentiviral expression of hCOL4A5 in mammalian cells

Depositor

Jeffrey Miner

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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Accessory plasmid 2

Plasmid

#128571

Purpose

for cloning identified individual SPECS

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagRFP

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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CelTag Plasmid

Plasmid

#66562

Purpose

Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

Depositor

David Engelke

Article

Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

Insert

CelTag

Expression

Bacterial

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-Lasp1-N-10

Plasmid

#55071

Purpose

Localization: Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Lasp1 (LASP1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

A209V

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
AiP1010-pAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA

Plasmid

#163476

Purpose

Direct-expressing iCre AAV Virus. Alias: AiP1010 - pAAV-AiE2004m-minBG-iCre-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

iCre

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-RfxCas13d-Blast-BFP

Plasmid

#196726

Purpose

Sortable and selectable RfxCas13d.

Depositor

Bernhard Schmierer

Insert

RfxCas13d-T2A-BSD-TagBFP

Use

CRISPR and Lentiviral

Tags

SV40 NLS

Promoter

EF1a

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-Cas9-VQR-Blast

Plasmid

#87155

Purpose

lentiviral expression of SpCas9-VQR (NGA PAM restricted)

Depositor

Daniel Bauer

Article

Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

Insert

SpCas9-VQR(D1135V,R1335Q,T1337R)

Use

Lentiviral

Mutation

D1135V,R1335Q,T1337R

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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