We narrowed to 7,257 results for: trac

1. pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

   Plasmid

   #45565

   Purpose

   Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   I-Sce I Nuclease
   BFP
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZac2.1 hSynapsin1 NAPA-N SV40

   Plasmid

   #92282

   Purpose

   FRET probe; extracellular synapse targeted mCherry

   Depositor

   Baljit Khakh

   Article

   Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

   Insert

   Neuron-astrocyte proximity assay - Neuronal
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSynapsin1

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCLE NICD3

   Plasmid

   #26894

   Depositor

   Nicholas Gaiano

   Article

   Dang et al Dev Neurosci. 2006;28(1-2):58-69.

   Insert

   NICD3 (NOTCH3 Human)
   

   Use

   Retroviral

   Tags

   Alkaline Phosphatase and IRES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-mCh-kCAAX

   Plasmid

   #112267

   Purpose

   ITRACK Acceptor Construct

   Depositor

   Ryohei Yasuda

   Article

   Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

   Insert

   mCherry with KRas membrane targeting domain
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ135B2.0

   Plasmid

   #167159

   Purpose

   Golden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU3

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ136B2.0

   Plasmid

   #167160

   Purpose

   Golden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU3

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFG036 + pFG051

   Plasmid

   #137997

   Purpose

   Transposon mutagenesis plasmid and the cI repressor expression plasmid

   Depositor

   Sébastien Rodrigue

   Article

   Neil et al Commun Biol. 2020 Sep 22;3(1):523. doi:

   Insert

   Tn5 tnp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC_sgRNA

   Plasmid

   #68710

   Purpose

   Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

   Depositor

   Roberto Docampo

   Article

   Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Human p150,95

   Plasmid

   #8633

   Depositor

   Timothy Springer

   Article

   Corbi et al EMBO J 1987 Dec 20;6(13):4023-8.

   Insert

   Human p150,95 (ITGAX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZac2.1 hSynapsin1 FLEX NAPA-N SV40

    Plasmid

    #92283

    Purpose

    FRET probe; extracellular synapse targeted mCherry, Cre-dependently expressed

    Depositor

    Baljit Khakh

    Article

    Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

    Insert

    Neuron-astrocyte proximity assay - Neuronal
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSynapsin1

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTol2-hspDRv7_scGstlt

    Plasmid

    #108870

    Purpose

    scGESTALT V7 lineage barcode for zebrafish

    Depositor

    Alex Schier

    Article

    Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

    Insert

    scGESTALT lineage tracing target V7
    

    Use

    Tol2 integration into zebrafish genome

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKS235

    Plasmid

    #191855

    Purpose

    Negative selection marker for MosSCI

    Depositor

    K Subramaniam

    Article

    Revathi et al MicroPubl Biol. 2022 Oct 20;2022:10.1791

    Insert

    peel-1 toxic protein for negative selection against extrachromosomal array
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    hsp-16.41

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWJ68

    Plasmid

    #46571

    Purpose

    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    w-dcas9
    CRISPR array
    

    Use

    CRISPR

    Tags

    rpoZ

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXD69m1-TetW

    Plasmid

    #191246

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid with tetracycline resistance in E. lenta

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAX APP C60

    Plasmid

    #30152

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein C60
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fragment

    Promoter

    Available since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pAH25-SceI

    Plasmid

    #129389

    Purpose

    Derived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Chloramphenicol acetyltransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    CAT promoter

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pME-Fv2E-flt4um200-ns

    Plasmid

    #67696

    Purpose

    middle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domain

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    Fv2E-flt4um200
    

    Use

    Tags

    HA

    Expression

    Mutation

    um200, no stop codon; N1056S

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSW002

    Plasmid

    #111248

    Purpose

    Broad host-range bacterial vector

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSW002-Pc

    Plasmid

    #108234

    Purpose

    Broad host bacterial expression vector with constitutive Pc promoter.

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    Pc promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBEVY-4420-mCherry

    Plasmid

    #218828

    Purpose

    Yeast surface display of an anti-fluorescene scFv and intracellular expression of mCherry

    Depositor

    Yongku Cho

    Article

    Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

    Insert

    4-4-20 scFv
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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