We narrowed to 677 results for: Bacillus

1. eA3A-BE3 (pRZ206)

   Plasmid

   #131315

   Purpose

   CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. hA3A-BE3 (pRZ215)

   Plasmid

   #131314

   Purpose

   CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. nCas9-UGI-NLS-P2A-EGFP (pJUL1001)

   Plasmid

   #123611

   Purpose

   CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   nCas9-UGI-NLS-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CAG

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. hAID-BE3 (pRZ352)

   Plasmid

   #131316

   Purpose

   CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYTRW27K_7Ti1

   Plasmid

   #177294

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and LacZ

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Insert

   PsacB-sacB
   

   Use

   Synthetic Biology; Yeast expression, rrn genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYTRW28K_0Ti1

   Plasmid

   #177290

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Insert

   PsacB-sacB
   

   Use

   Synthetic Biology; Yeast expression, rrn genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAcBac1-EcTyr-TAG

   Plasmid

   #228782

   Purpose

   EcTyr aaRS/tRNA system for mammalian ncAA incorporation

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTyrRS
   EcYtR
   BstR
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Promoter

   Available Since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28:LanIII-6

   Plasmid

   #225076

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanIII-6 (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA(A) (LanIII-6)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDR111_mannose_Stat1Klf6

   Plasmid

   #188400

   Purpose

   Expresses mouse transcription factors Stat-1 and Klf6 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.

   Depositor

   Christopher Contag

   Article

   Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

   Inserts

   Stat-1 (Stat1 Mouse, Escherichia coli)
   Klf6 (Klf6 Mouse)
   

   Use

   Tags

   PhoD secretion peptide

   Expression

   Bacterial

   Mutation

   Promoter

   D-mannose

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. C85m

    Plasmid

    #120996

    Purpose

    MoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    Sigma X (ECF20_992)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBsacA

    Plasmid

    #158650

    Purpose

    Constitutive transcription of sacA crRNA

    Depositor

    Wenjing Cui

    Insert

    sacA crRNA
    

    Use

    CRISPR and Synthetic Biology; Shuttle vector baci…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pveg

    Available Since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHTganA-Cpf1

    Plasmid

    #158647

    Purpose

    Constitutive transcription of FnCpf1 and ganA crRNA

    Depositor

    Wenjing Cui

    Insert

    Cpf1, ganA crRNA
    

    Use

    CRISPR and Synthetic Biology; Shuttle vector baci…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P43

    Available Since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pADsacA

    Plasmid

    #158649

    Purpose

    Constitutive transcription of sacA crRNA and GFP

    Depositor

    Wenjing Cui

    Insert

    sacA crRNA
    

    Use

    CRISPR and Synthetic Biology; Shuttle vector baci…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pveg

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCIRCgp41-1

    Plasmid

    #74227

    Purpose

    Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    gp41-1 C-intein and gp41-1 N-intein

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCIRCNpu

    Plasmid

    #74226

    Purpose

    Vector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Npu DnaE C-intein and Npu DnaE N-intein

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

    Plasmid

    #123615

    Purpose

    CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3(R33A/K34A)-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A/K34A in rAPOBEC1, D10A in SpCas9

    Promoter

    CAG

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

    Plasmid

    #123614

    Purpose

    CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3(R33A)-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in SpCas9

    Promoter

    CAG

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGV3319

    Plasmid

    #170277

    Purpose

    Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

    Depositor

    Gregory Van Duyne

    Article

    Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

    Insert

    Bst-LF D720A
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    D720A

    Promoter

    T7-lac

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. BE3(E63Q)-P2A-EGFP (pRZ189)

    Plasmid

    #123613

    Purpose

    CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3(E63Q)-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E63Q in rAPOBEC1, D10A in SpCas9

    Promoter

    CAG

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSS02:cyt-Peredox-mCherry

    Plasmid

    #161743

    Purpose

    cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

    Depositor

    Markus Schwarzländer

    Article

    Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

    Insert

    cyt-Peredox-mCherry
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubiquitin 10

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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