We narrowed to 9,859 results for: Coli

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psgRNA

Plasmid

#114005

Purpose

express sgRNA. ColE1, Kan

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Insert

gRNA

Promoter

BBa_J23119

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pTrcECT

Plasmid

#75477

Purpose

Inducible expression of ectABC in E. coli

Depositor

Xixian Xie

Article

Ning et al Metab Eng. 2016 Mar 9;36:10-18. doi: 10.

Insert

ectABC

Expression

Bacterial

Promoter

trc

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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psgRNAc

Plasmid

#114006

Purpose

express sgRNA, p15A, CRM

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Insert

gRNA

Promoter

BBa_J23119

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMBI

Plasmid

#17816

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
TGT-His

Plasmid

#138201

Purpose

Expresses E. Coli TGT with a C-terminal His Tag

Depositor

Neal Devaraj

Article

Busby et al Methods Enzymol. 2020;641:373-399. doi:

Insert

E Coli tRNA Guanine Transglycosylase-His

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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PIA900

Plasmid

#104401

Purpose

Polycistronic vector for expression of E. coli core RNAP

Depositor

Irina Artsimovitch

Article

Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

Insert

rpoA, B, C, and Z

Use

E. coli expression vector

Tags

encodes a C-terminally His-tagged rpoC which can …

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAB172a

Plasmid

#156324

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Mutation

WT antiRBS engineered to orthogonal antiRBS

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-FRT

Plasmid

#183241

Purpose

anhydrotetracycline (aTC) inducible sgRNA that targets FRT scar sites and arabinose inducible lambda Red

Depositor

Karin Schnetz

Article

Rangarajan et al Microbiology (Reading). 2022 Apr;168(4).

Insert

sgRNA-FRT

Expression

Bacterial

Promoter

P-tet

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLA230

Plasmid

#11724

Depositor

Lawrence Loeb

Article

Shinkai et al J Biol Chem. 2001 Dec 14. 276(50):46759-

Insert

b-lactamase

Expression

Bacterial

Mutation

GAA for Glu26 changed to TAA (premature stop codo…

Available Since

Aug. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pHK316

Plasmid

#235474

Purpose

Inducible gene VIII by Ptet promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII

Expression

Bacterial

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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PAS528 (TJF059)

Plasmid

#65602

Purpose

pETDuet-1 Containing FadD-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Tags

6x His

Expression

Bacterial

Promoter

T7 lacO

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pTETDuet_M42FG121VDHFR_R166QTS

Plasmid

#191298

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

M42F/G121V DHFR, R166Q TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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EcYbbN pET15b

Plasmid

#60690

Purpose

Recombinant protein expression of YbbN

Depositor

Mark Wilson

Article

Lin et al J Biol Chem. 2011 Jun 3;286(22):19459-69

Insert

YbbN

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pMAM-RecG-ST2TaV-EGFP

Plasmid

#31275

Depositor

Andrew Pierce

Article

Killen et al BMC Mol Biol. 2012 Oct 30;13:33. doi: 10

Insert

RecG-ST2-TaV-EGFP

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pMAM-RecG-EGFP

Plasmid

#31274

Depositor

Andrew Pierce

Article

Killen et al BMC Mol Biol. 2012 Oct 30;13:33. doi: 10

Insert

RecG-EGFP

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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MetNI (Lm)

Plasmid

#78316

Purpose

Methionine ABC Transporter

Depositor

Douglas Rees

Article

Kadaba et al Science. 2008 Jul 11;321(5886):250-3. do

Inserts

MetI
MetN

Tags

10x Histidine Tag and 6x Histidine Tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD33(A-N)

Plasmid

#187084

Purpose

Medium expression of Complex I with subunit N-HA tagged

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoA-N

Tags

HA tag nuoN

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD33(EFG)

Plasmid

#187087

Purpose

Medium expression of subunits EFG

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoEFG

Tags

HA tag nuoG

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAW209

Plasmid

#113188

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pTETDuet_WTDHFR_WTTS

Plasmid

#191282

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

WT DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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