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We narrowed to 1,350 results for: RPL

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Showing: 421 - 440 of 1350 results

  1. pIEforRed

    Plasmid
    #185892
    Purpose
    In vivo gene amplification (HamAmp) of EforRed expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185892-plasmid-map-sequence-id-370865

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  2. pUC-ODN2

    Plasmid
    #208643
    Purpose
    Empty vector for long single strand DNA (up to 1.5k) preparation. Further remove BsaI and BsmBI site in pUC-ODN (Addgene #208642).
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Long single strand dna production
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208643-plasmid-map-sequence-id-418356

    Enlarge

  3. pcDNA3.1-3'3xFlag

    Plasmid
    #208616
    Purpose
    Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208616-plasmid-map-sequence-id-418312

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  4. pcDNA3.1-StrepII

    Plasmid
    #208617
    Purpose
    Empty vector for mammalian expression, insert a Strep II tag (with ATG) using XbaI and XhoI site of pcDNA3.1 (-), both site retained
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Strep II
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208617-plasmid-map-sequence-id-418313

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  5. pIAeBlue

    Plasmid
    #185891
    Purpose
    In vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185891-plasmid-map-sequence-id-370863

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  6. pIEforRed

    Plasmid
    #185892
    Purpose
    In vivo gene amplification (HamAmp) of EforRed expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185892-plasmid-map-sequence-id-370865

    Enlarge

  7. pET28-H12TEV

    Plasmid
    #208635
    Purpose
    for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    6xhis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208635-plasmid-map-sequence-id-433640

    Enlarge

  8. pcDNA3.1-3'TwStrep

    Plasmid
    #208618
    Purpose
    Empty vector for mammalian expression, insert Twin Strep tag using BamHI and AflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with Twin Strep tag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Twin Strep
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208618-plasmid-map-sequence-id-418314

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  9. pUC-ODN

    Plasmid
    #208642
    Purpose
    Empty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Long single strand dna production
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208642-plasmid-map-sequence-id-418350

    Enlarge

  10. pET28-3'TwStrep

    Plasmid
    #208053
    Purpose
    Empty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Twin Strep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208053-plasmid-map-sequence-id-415923

    Enlarge

  11. pET28-5'TwStrep

    Plasmid
    #208620
    Purpose
    Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Twin Strep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208620-plasmid-map-sequence-id-415919

    Enlarge

  12. pUC-ODN2

    Plasmid
    #208643
    Purpose
    Empty vector for long single strand DNA (up to 1.5k) preparation. Further remove BsaI and BsmBI site in pUC-ODN (Addgene #208642).
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Long single strand dna production
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208643-plasmid-map-sequence-id-418356

    Enlarge

  13. pcDNA3.1-Flag

    Plasmid
    #208051
    Purpose
    Empty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with Flag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208051-plasmid-map-sequence-id-418310

    Enlarge

  14. pET28-Flag-RBM24

    Plasmid
    #208621
    Purpose
    for E. coli expression of Flag tagged human RBM24 (ENST00000379052)
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000379052) coding region (RBM24 Human)
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208621-plasmid-map-sequence-id-433637

    Enlarge

  15. pcDNA3.1-3'3xFlag

    Plasmid
    #208616
    Purpose
    Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208616-plasmid-map-sequence-id-418312

    Enlarge

  16. pcDNA3.1-StrepII

    Plasmid
    #208617
    Purpose
    Empty vector for mammalian expression, insert a Strep II tag (with ATG) using XbaI and XhoI site of pcDNA3.1 (-), both site retained
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Strep II
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208617-plasmid-map-sequence-id-418313

    Enlarge

  17. pcDNA3.1-Flag-RBM24

    Plasmid
    #208052
    Purpose
    Expressing N-ter Flag tagged human RBM24 (ENST00000379052) in mammalian cells
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000379052) coding region (RBM24 Human)
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208052-plasmid-map-sequence-id-418311

    Enlarge

  18. pET28-Flag-RBM24-1~93

    Plasmid
    #208633
    Purpose
    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    only aa 1~93, other residues not included
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208633-plasmid-map-sequence-id-415921

    Enlarge

  19. pET28-H12TEV

    Plasmid
    #208635
    Purpose
    for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    6xhis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208635-plasmid-map-sequence-id-433640

    Enlarge

  20. pcDNA3.1-3'TwStrep

    Plasmid
    #208618
    Purpose
    Empty vector for mammalian expression, insert Twin Strep tag using BamHI and AflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with Twin Strep tag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Twin Strep
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208618-plasmid-map-sequence-id-418314

    Enlarge

Showing: 421 - 440 of 1350 results