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Viral prep#98928-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 (#98928). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent expression of Ruby2sm-Flag spaghetti monster construct. Spaghetti monster constructs are not fluorescent ("dark") to allow full range of antibody staining. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmRuby2sm-Flag (spaghetti monster construct)Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only
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pNH-TrxT
Plasmid#26106PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneTags6xHis, TEV cleavage site, and TrxAExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available sinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-nEF-Coff/Fon-ChRmine-oScarlet (AAV8)
Viral prep#137160-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChRmine-oScarlet (#137160). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChRmine-oScarlet plasmid DNA. nEF-driven expression of ChRmine-oScarlet in the presence of Flp (inhibited in the presence of Cre). These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsoScarlet (Flp-dependent)Available sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Con/Fon-GCaMP6M
Plasmid#137119PurposeIntersectional viral expression of GCaMP6M in cells expressing both Cre AND FlpDepositorHas serviceAAV8InsertCon/Fon-GCaMP6M
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationRemoved RSET tagPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Ef1a-Con/Fon-GCaMP6F
Plasmid#137122PurposeIntersectional viral expression of GCaMP6F in cells expressing both Cre AND FlpDepositorHas serviceAAV5 and AAV8InsertCon/Fon-GCaMP6F
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationRemoved RSET tagPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
anti-FLAG M2 light chain
Plasmid#175358PurposeMammalian expression vector for over-expression of anti-FLAG M2 light chainDepositorInsertanti-FLAG M2 light chain
TagsN-terminal secretion signal peptide (MEAPAQLLFLLL…ExpressionMammalianPromoterCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-MP2
Plasmid#36097Purpose3rd generation lentiviral plasmidDepositorTypeEmpty backboneUseLentiviralAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-nEF-Con/Fon-ChRmine-oScarlet
Plasmid#137159PurposeIntersectional viral expression of ChRmine-p2a-oScarlet in cells expressing both Cre AND FlpDepositorHas serviceAAV8InsertCon/Fon-ChRmine-p2a-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationoScarlet E95DPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-CMV-jGCaMP7f
Plasmid#104483PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertjGCaMP7f
TagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Ef1a-mCherry-IRES-Cre (AAV Retrograde)
Viral prep#55632-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-Ef1a-mCherry-IRES-Cre (#55632). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry-IRES-Cre plasmid DNA. Cre and bicistronic (IRES) mCherry expression under the EF1a promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1a and Ef1a/IRESTagsmCherryAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-mCherry-IRES-Cre
Plasmid#55632PurposeExpresses Cre in Mammalian CellsDepositorHas serviceAAV Retrograde and AAV8InsertsmCherry
Cre
UseAAVExpressionMammalianPromoterEf1a and Ef1a/IRESAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRGEN-CMV-CjCas9
Plasmid#89752PurposeExpression of CjCas9 in mammalian cellsDepositorInsertCas9
UseCRISPRTagsSV40-HAExpressionMammalianAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGRB
Plasmid#71539Purposefor expression of gRNA in E. coliDepositorTypeEmpty backboneExpressionBacterialPromoterJ231196Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pREDCas9
Plasmid#71541PurposeConstitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
lambda red genes
plasmid curing system
ExpressionBacterialPromoterpBADAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQCXIP-BSR-GFP11
Plasmid#68716PurposeSplit GFP assayDepositorInsertbsr
UseRetroviralTagsFLAG and GFP11ExpressionMammalianAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
anti-FLAG M2 heavy chain
Plasmid#175359PurposeMammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)DepositorInsertanti-FLAG M2 heavy chain
TagsHis8 tag and N-terminal secretion signal peptide …ExpressionMammalianMutationL51I (see paper)PromoterCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet
Plasmid#137161PurposeIntersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Cre AND NOT FlpDepositorHas serviceAAV8InsertCon/Foff 2.0-ChRmine-p2a-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationoScarlet E95DPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDULE-ABK
Plasmid#49086PurposeA pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experimentsDepositorPublicationInsertstRNA synthetase
tRNA for a photocrosslinking amino acid
ExpressionBacterialMutationL274M, C313A, Y349FPromoterGlnRS and lppAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
Available sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-mTH-GFP
Plasmid#99128PurposeAn AAV genome that expresses GFP from the mouse TH promoterDepositorInsertGFP
UseAAVExpressionMammalianPromotermTHAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by