We narrowed to 6,241 results for: nls

1. pHR'CMVGFP_NLS_hRIF1(1-1308)

   Plasmid

   #23134

   Depositor

   Lifeng Xu

   Article

   Xu et al J Cell Biol. 2004 Dec 6. 167(5):819-30.

   Insert

   RIF1 deletion construct (RIF1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Feb. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNLS-iRFP713

   Plasmid

   #45468

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP713
   

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   T2A, S13L, A92T, V104T, V114I, E161K, E193K, F198…

   Promoter

   CMV

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGPTVII-nlsSED1

   Plasmid

   #178190

   Purpose

   SED1 biosensor for expression in Arabidopsis thaliana

   Depositor

   Jose Dinneny, Cesar Cuevas-Velazquez

   Article

   Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

   Insert

   SED1 biosensor
   

   Tags

   SV40 NLS

   Expression

   Plant

   Promoter

   pUBQ10

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-P4:gs:nls:CIBN

   Plasmid

   #135986

   Purpose

   Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   P4 coiled-coil fused to the CIBN domain
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNLS-iRFP670

   Plasmid

   #45466

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP670
   

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

   Promoter

   CMV

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. p6XHis_NLS-SaCas9

   Plasmid

   #101086

   Purpose

   Express SaCas9 in bacteria with a 6xHis tag for purification

   Depositor

   Rick Tarleton

   Article

   Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

   Insert

   CRISPR-associated protein Cas9/Csn1
   

   Use

   CRISPR

   Tags

   TrxA-6xHis-STag

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWPT-nlsLacZ

   Plasmid

   #12261

   Depositor

   Didier Trono

   Insert

   LacZ
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_EF1a-->GssmycLaG16LaG2_P2A_NLSIFP

   Plasmid

   #162228

   Purpose

   Lentiviral vector for the expression of Myc GFP nanbody LaG16LaG2 with IFP NLS in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   LaG16LaG2 and IFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-Bxb1o_nlsFlag

   Plasmid

   #119901

   Purpose

   Expresses Bxb1 integrase in mammalian cells

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   Mammalian codon optimized Bxb1 integrase
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. L_NLS 41kDa

    Plasmid

    #201342

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    (NLS)SV40A4-EGFP-2PrA
    

    Expression

    Mammalian

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. nls-NgAgo-GK

    Plasmid

    #78253

    Purpose

    Mammalian expression of NgAgo (Argonaute from N. gregoryi).

    Depositor

    Chunyu Han

    Article

    Gao et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    NgAgo
    

    Tags

    a nuclear localization signal (NLS)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. 4xNLS-pMJ915v2

    Plasmid

    #88917

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. p16 nlsGPS2

    Plasmid

    #228093

    Purpose

    Expression plasmid for genetically-encoded FRET biosensor for Gibberellin (GA)

    Depositor

    Alexander Jones

    Article

    Griffiths et al Plant Cell. 2024 Jul 23:koae198. doi: 10

    Insert

    nlsGPS2
    

    Tags

    6xHis-T7 XpressTag and cMyc

    Expression

    Plant

    Promoter

    p16

    Available Since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNLS-iRFP720

    Plasmid

    #45467

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP720
    

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3xnls-mTurquoise2

    Plasmid

    #98817

    Purpose

    In vivo visualization of the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    3xNLS
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. AsCpf1-2NLS

    Plasmid

    #102565

    Purpose

    bacterial expression of AsCpf1-2NLS

    Depositor

    Jennifer Doudna

    Article

    Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

    Insert

    AsCpf1
    

    Tags

    6xHis-MBP-TEV and HA-2xSV40NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. M_NLS 41kDa

    Plasmid

    #201341

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    (NLS)SV40A5-EGFP-2PrA
    

    Expression

    Mammalian

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-21a_FnCpf1_2xNLS_protein_expression

    Plasmid

    #124548

    Purpose

    Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnCpf1_2xNLS
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNLS-2xBFP(4C)

    Plasmid

    #176151

    Purpose

    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    NLS-2xBFP(4C)
    

    Tags

    6xHis tag and SV40 nuclear localization sequence …

    Expression

    Bacterial

    Mutation

    Compared with EGFP, the two BFP domains have the …

    Promoter

    Ptrc

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-NLS-HA-B3

    Plasmid

    #51270

    Purpose

    Expression of the site-specific recombinase B3 (mouse codon-optimized)

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    NLS-HA-B3
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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