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pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)

Plasmid

#114081

Purpose

CAG promoter expression plasmid for human codon optimized enAsBE1.1

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1)

Tags

SV40 NLS

Expression

Mammalian

Mutation

E174R, S542R, K548R and D908A, human codon optimi…

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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MF81_PB-TRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

Plasmid

#176625

Purpose

TF A self-activation cassette in MultiFate-2.1

Depositor

Michael Elowitz

Article

Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

Insert

TRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

Use

Synthetic Biology

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a_eeBxb1_Int_2x_NPL_c-term_NLS_pTwist

Plasmid

#244901

Purpose

Expression of eeBxb1

Depositor

Andrew Bassett

Article

Migliori et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

eeBxb1

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP670

Plasmid

#45466

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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3xnls-mScarlet-I

Plasmid

#98816

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

3xNLS

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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3xnls-mTurquoise2

Plasmid

#98817

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

3xNLS

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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4xNLS-pMJ915v2

Plasmid

#88917

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Bxb1o_nlsFlag

Plasmid

#119901

Purpose

Expresses Bxb1 integrase in mammalian cells

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

Mammalian codon optimized Bxb1 integrase

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBPnlsLexA::p65Uw

Plasmid

#26230

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

nlsLexA::p65 (RELA Human)

Expression

Insect

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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3xnls-mNeonGreen

Plasmid

#98875

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

3xNLS

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET-21a_3xNLS_SpCas9_protein_expression

Plasmid

#114365

Purpose

Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

Depositor

Scot Wolfe

Article

Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

Insert

3xNLS_SpCas9

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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p3xnls-mScarlet3_N1

Plasmid

#189775

Purpose

Construct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

3xnls-mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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p16 nlsGPS1

Plasmid

#101081

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Article

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

nlsGPS1

Tags

cMyc

Expression

Plant

Promoter

p16 (AT3G60245)

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_Cas9–superNLS

Plasmid

#232430

Purpose

Expression plasmid for the SpCas9 nuclease with superNLS

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

Cas9–superNLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBPnlsLexA::GADflUw

Plasmid

#26232

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

nlsLexA::GADfl

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV_ABE8e–superNLS

Plasmid

#232429

Purpose

Expression plasmid for the ABE8e base editor with superNLS

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

ABE8e–superNLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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L_NLS 41kDa

Plasmid

#201342

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A4-EGFP-2PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP720

Plasmid

#45467

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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AAV_Efs_hSpCas9_NLS_FLAG-SV40

Plasmid

#97307

Purpose

AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

humanized S. pyogenes Cas9

Use

AAV and Mouse Targeting

Tags

Flag

Expression

Mammalian

Promoter

EFS

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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p16 nlsGPS2

Plasmid

#228093

Purpose

Expression plasmid for genetically-encoded FRET biosensor for Gibberellin (GA)

Depositor

Alexander Jones

Article

Griffiths et al Plant Cell. 2024 Jul 23:koae198. doi: 10

Insert

nlsGPS2

Tags

6xHis-T7 XpressTag and cMyc

Expression

Plant

Promoter

p16

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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