We narrowed to 6,134 results for: nls

1. AsCpf1-2NLS

   Plasmid

   #102565

   Purpose

   bacterial expression of AsCpf1-2NLS

   Depositor

   Jennifer Doudna

   Article

   Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

   Insert

   AsCpf1
   

   Use

   Tags

   6xHis-MBP-TEV and HA-2xSV40NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. nls-NgAgo-GK

   Plasmid

   #78253

   Purpose

   Mammalian expression of NgAgo (Argonaute from N. gregoryi).

   Depositor

   Chunyu Han

   Article

   Gao et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   NgAgo
   

   Use

   Tags

   a nuclear localization signal (NLS)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. M_NLS 41kDa

   Plasmid

   #201341

   Purpose

   Nucleocytoplasmic transport probe

   Depositor

   Pere Roca-Cusachs

   Article

   Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

   Insert

   (NLS)SV40A5-EGFP-2PrA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNLS-iRFP720

   Plasmid

   #45467

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP720
   

   Use

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

   Promoter

   CMV

   Available since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-21a_FnCpf1_2xNLS_protein_expression

   Plasmid

   #124548

   Purpose

   Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   FnCpf1_2xNLS
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xnls-mTurquoise2

   Plasmid

   #98817

   Purpose

   In vivo visualization of the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   3xNLS
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-NLS-HA-B3

   Plasmid

   #51270

   Purpose

   Expression of the site-specific recombinase B3 (mouse codon-optimized)

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   NLS-HA-B3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-P4:gs:nls:CIBN

   Plasmid

   #135986

   Purpose

   Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   P4 coiled-coil fused to the CIBN domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40

   Plasmid

   #100030

   Purpose

   AAV expression of ArcLightD

   Depositor

   Lawrence Cohen

   Article

   Storace et al Sci Rep. 2015 May 13;5:10212. doi: 10.10

   Insert

   ArcLightD
   

   Use

   AAV

   Tags

   2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBA

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNLS-2xBFP(4C)

    Plasmid

    #176151

    Purpose

    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    NLS-2xBFP(4C)
    

    Use

    Tags

    6xHis tag and SV40 nuclear localization sequence …

    Expression

    Bacterial

    Mutation

    Compared with EGFP, the two BFP domains have the …

    Promoter

    Ptrc

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGPTVII-nlsSED1

    Plasmid

    #178190

    Purpose

    SED1 biosensor for expression in Arabidopsis thaliana

    Depositor

    Cesar Cuevas-Velazquez, Jose Dinneny

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 biosensor
    

    Use

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    pUBQ10

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. L_NLS 67kDa

    Plasmid

    #201361

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    (NLS)SV40A4-EGFP-6PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. H_NLS 67kDa

    Plasmid

    #201372

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    (NLS)SV40-EGFP-6PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. p16 nlsGPS1

    Plasmid

    #101081

    Purpose

    GA biosensor expression plasmid

    Depositor

    Alexander Jones, Wolf Frommer

    Article

    Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

    Insert

    nlsGPS1
    

    Use

    Tags

    cMyc

    Expression

    Plant

    Mutation

    Promoter

    p16 (AT3G60245)

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRIPZ (M)-HT-NLS

    Plasmid

    #82518

    Purpose

    Lentivirus vector. Expresses Halotag proteins with nuclear localization signal in mammalian cells.

    Depositor

    Xiaojun Ren

    Insert

    Nuclear localizaition sequence
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSynapsin_NLS_GFP-P2A_SNAP_GluA2

    Plasmid

    #170455

    Purpose

    Expresses SNAP tagged GluA2 in neuronal cells

    Depositor

    Shigeki Watanabe

    Article

    Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

    Insert

    GluA2 (Gria2 Mouse)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. WT A1-LCD +NLS

    Plasmid

    #178658

    Purpose

    Bacterial expression of N-terminally 6His tagged WT A1-LCD (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    WT A1-LCD +NLS (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR_TRE-->2xNLS_IFP_PGK_PuroR

    Plasmid

    #162238

    Purpose

    Lentiviral vector for the expression of TRE-->2xNLS_IFP_PGK_PuroR in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    NLS IFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR_EF1a-->GssmycLaG16LaG2_P2A_NLSIFP

    Plasmid

    #162228

    Purpose

    Lentiviral vector for the expression of Myc GFP nanbody LaG16LaG2 with IFP NLS in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    LaG16LaG2 and IFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV_Efs_hSpCas9_NLS_FLAG-SV40

    Plasmid

    #97307

    Purpose

    AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    humanized S. pyogenes Cas9
    

    Use

    AAV and Mouse Targeting

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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