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pUCBB-ctH6-eGFP

Plasmid

#32562

Depositor

Claudia Schmidt-dannert

Publication

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Promoter

Consitutive lac

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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gCH29 (crCD81-1)

Plasmid

#217344

Purpose

crRNA targeting CD81

Depositor

Luke Gilbert

Publication

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

crCD81-1 (CD81 Human)

Use

CRISPR and Lentiviral

Promoter

hU6

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-AsCas12f1-v5.1+T1-empty

Plasmid

#204977

Purpose

AsCas12f1-v5.1+T1-based Human cell genome editing

Depositor

Quanjiang Ji

Publication

Wu et al Nature Catalysis (2023) 6, 695–709

Type

Empty backbone

Use

CRISPR

Mutation

N70Q/K103R/A104R/S118A/D364R

Promoter

PCMV,U6

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2 mBAK

Plasmid

#129578

Purpose

CRISPR deletion of murine BAK

Depositor

Stephen Tait

Publication

Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

Insert

gRNA vs murine BAK (Bak1 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

None

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC-EsaR-I70V-EsaI

Plasmid

#47661

Purpose

esaI gene downstream of esaR-a208g in pAC-EsaR-I70V (CmR)

Depositor

Cynthia Collins

Publication

Shong et al ACS Synth Biol. 2013 Jul 23.

Insert

esaI-term

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plac

Available since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pU6_rd6_pegRNA_(PP7-C4-Q1)

Plasmid

#232433

Purpose

pegRNA with optimized 3' modifications to correct the rd6 mutation

Depositor

Gil Westmeyer

Publication

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

3'-PP7-tagged pegRNA for rd6 correction driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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eiABE

Plasmid

#227144

Purpose

Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain was inserted between TadA-8e and eIscBn driven by CMV promoter

Depositor

Dali Li

Publication

Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

Insert

bpNLS-TadA-8e-linker-HMG-D-linker-nOgeuIscB-v3-bpNLS

Mutation

D96R/E84R/V159R/H339A

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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iCAP-BI-28-KanR

Plasmid

#203532

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Publication

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

AAV-BI28 Cap

Use

AAV

Expression

Mammalian

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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eTd-CBEm

Plasmid

#196602

Purpose

Mammalian expression of eTd-CBEm

Depositor

Dali Li

Publication

Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

Insert

bpNLS-TadA-8e (N46L/A48M)-nSpCas9-bpNLS-P2A-UGI-bpNLS

Tags

6*His

Expression

Mammalian

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-UXPS

Plasmid

#172138

Purpose

bacteria expression plasmid for a UDP/UTP sensor

Depositor

Huiwang Ai

Publication

Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

Insert

UXPS

Expression

Bacterial

Promoter

araBAD

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AasgRNA

Plasmid

#121954

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Publication

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA

Expression

Mammalian

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJY3948

Plasmid

#113338

Purpose

6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

Depositor

Suzanne Sandmeyer

Publication

Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

Insert

2-pyrone synthase, codon optimized for Y. lipolytica

Tags

6XHis

Expression

Yeast

Mutation

Please see depositor comments

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUASTattb-cAMPr Y205A, R626A

Plasmid

#84932

Purpose

cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.

Depositor

Justin Blau

Publication

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr Y205A R626A

Expression

Insect

Mutation

PKA-C Y205A, PKA-R R626A

Promoter

5X UAS

Available since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-miRE_shBRD9_508-SV40-GFP

Plasmid

#75138

Purpose

Retroviral expression plasmid encoding shBRD9_508 (targeting human BRD)

Depositor

Christopher Vakoc

Publication

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBRD9_508

Use

Retroviral

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGGE001

Plasmid

#48839

Purpose

Provides the rbcS terminator as GreenGate module.

Depositor

Jan Lohmann

Publication

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

rbcS terminator

Use

Golden gate compatible cloning vector

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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placZα-tdMCP

Plasmid

#191132

Purpose

Expression of tdMCP from a lacZα inducible promoter

Depositor

Ariel Lindner

Publication

Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

Insert

dMCP_1

Use

Synthetic Biology

Promoter

lac-inducible promoter

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas11

Plasmid

#178882

Purpose

Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag

Depositor

Yan Zhang

Publication

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas11c-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hGFAP-Pink Flamindo-WPRE-SV40

Plasmid

#178790

Purpose

AAV vector to express the red cAMP indicator in astrocytes

Depositor

Tetsuya Kitaguchi

Publication

Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

Insert

Pink Flamindo

Use

AAV

Promoter

hGFAP

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLIC-lyn11-myc-LOV2GIVe

Plasmid

#170623

Purpose

For the mammalian expression of mLOV2GIVe

Depositor

Mikel Garcia-Marcos

Publication

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe

Tags

myc

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCFJ2359 - intron GG2 - 250bp

Plasmid

#159878

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG2 - 250bp

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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