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LI B-C c-myc

Plasmid

#54450

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

c-MYC epitope tag (EQKLISEEDL)

Use

Cloning vector

Available Since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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LI D-E mCherry + link c1

Plasmid

#54420

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + mCherry

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pUHD-SB-AID/Hyg

Plasmid

#171677

Purpose

TRE-controlled AID-fusion sleeping beauty transposon vector with selection marker (hygromycin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Type

Empty backbone

Tags

AID

Expression

Mammalian

Promoter

TRE

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-stl

Plasmid

#51721

Purpose

conditional gene knockdowns in Trypanosoma brucei

Depositor

Arthur Gunzl

Article

Brandenburg et al EMBO J. 2007 Nov 28;26(23):4856-66. Epub

Type

Empty backbone

Use

Conditional gene knockdown in t. brucei

Promoter

T7 (2X)

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pPERPlucFL

Plasmid

#14811

Depositor

Tyler Jacks

Article

Reczek et al Mol Cancer Res. 2003 Dec. 1(14):1048-57.

Insert

PERP (Perp Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
phlFs-mLin

Plasmid

#140792

Purpose

CMV-phlFo-phlFR-2A-mCherry mammalian synthetic gene circuit

Depositor

Gabor Balazsi

Article

Szenk et al ACS Synth Biol. 2020 Apr 17;9(4):930-939

Insert

phlFs-mLinearizer

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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BSP179

Plasmid

#110915

Purpose

For cloning expression cassettes by HR in yeast, Universal MosSCI compatible

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Type

Empty backbone

Use

Synthetic Biology; Yeast homologous recombination…

Expression

Worm

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pME-nEGFP

Plasmid

#138363

Purpose

Gateway compatible middle entry clone contains nuclear EGFP sequences.

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

nEGFP

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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LI C-D 11GFP

Plasmid

#54431

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

superfolder GFP 11 domain

Use

Cloning vector

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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LI A-B Ubi (+ intron) c4

Plasmid

#54408

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

L. japonicus polyubiquitin promoter

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-CMVmin:GFPmem pA

Plasmid

#80823

Purpose

CMVmin-promoted GFPmem with pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

3E-CMV-GFPCAAXpA spacer

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pME-mVenus-NS

Plasmid

#80497

Purpose

Multisite gateway entry clone for adding N-terminal fusions of mVenus

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

mVenus (no STOP)

Use

Multisite gateway middle entry clone

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET26-CNTN149L

Plasmid

#100164

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Mutation

N149L

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p70rg 1

Plasmid

#17828

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
mEGFP-30nmSAH-IQ

Plasmid

#61678

Purpose

GFP-labeled SAH scaffold with IQ attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-IQ

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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LI C-D destab GFP c1

Plasmid

#54454

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

destablized GFP

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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mEGFP-EF(N)-30nmSAH

Plasmid

#61679

Purpose

GFP-labeled SAH scaffold with EF Hand attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-EF(N)-30nmSAH

Expression

Mammalian

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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LI C-D Cerulean c1

Plasmid

#54417

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

CFP Cerulean

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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p70rg 5

Plasmid

#17829

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pLIC.B3.TM1734

Plasmid

#11527

Depositor

Sung-Hou Kim

Article

Liu et al J Biol Chem. 2005 Apr 22. 280(16):15960-

Insert

PhoU (TM1734 T. maritima)

Tags

HisTag, TEV cleavable

Expression

Bacterial

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only