We narrowed to 11,762 results for: pcDNA

1. pcDNA pDEST40 Cas9-POLD3

   Plasmid

   #183194

   Purpose

   Expression clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   POLD3
   

   Use

   CRISPR

   Tags

   Cas9WT

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1/myc-his Ndel1

   Plasmid

   #12572

   Depositor

   Li-Huei Tsai

   Article

   Shu et al Neuron. 2004 Oct 14. 44(2):263-77.

   Insert

   Ndel1 (NDEL1 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1-hRIPK1-Flag-delDD

   Plasmid

   #112494

   Purpose

   Mammalian expression of the indicated Flag- hRIPK1 mutant. Please note Flag tag is N-terminal.

   Depositor

   Junying Yuan

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deletion of DD domain (AA584-671 deleted)

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)/UUG-nLuc-3XFLAG

   Plasmid

   #127302

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from UUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. CHOP 6: mCHOP-WT-9E10-pcDNA1

   Plasmid

   #21913

   Depositor

   David Ron

   Insert

   CHOP10 (Ddit3 Mouse)
   

   Use

   Tags

   9E10 (Myc)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-BG4-NLS-mVen

   Plasmid

   #211449

   Purpose

   mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence

   Depositor

   Helmut Pospiech

   Insert

   Anti-DNA G-quadruplex single chain antibody BG4
   

   Use

   Tags

   NLS-mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4/HisMaxB-YAP1-S127A

   Plasmid

   #18988

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Jul 17. ():.

   Insert

   Yes-kinase associated protein S127A mutant (YAP1 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Serine 127 changed to Alanine, mutated protein t…

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA5/FRT/TO-GAlphaGustducin-RLuc8

   Plasmid

   #140979

   Purpose

   Encodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphaGustducin-RLuc8 (GNAT3 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5/FRT/TO_CEP41-HaloTag7_T2A_EGFP

   Plasmid

   #169327

   Purpose

   Expression of CEP41-HaloTag7 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   CEP41-HaloTag7 (CEP41 Human)
   

   Use

   Tags

   HaloTag7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Tras light chain

    Plasmid

    #216294

    Purpose

    Expresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Tras light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Pert Light chain

    Plasmid

    #216310

    Purpose

    Expresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Pert Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPD005 pcDNA Golden Gate

    Plasmid

    #138749

    Purpose

    Vector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-Ikkb-FLAG(K44A)

    Plasmid

    #23299

    Depositor

    Warner Greene

    Article

    Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

    Insert

    Ikkbeta (IKBKB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    Available since

    April 16, 2010

    Availability

    Academic Institutions and Nonprofits only

14. LARG mtq sspB in pcDNA3.1

    Plasmid

    #90460

    Purpose

    RhoA mediated cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

    Inserts

    LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
    mTq
    sspB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R73Q and deletion a.a. (234-239)

    Promoter

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO GFP DNAJB9

    Plasmid

    #19505

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DNAJB9 (DNAJB9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-FRT-TO-C-8His-Flag

    Plasmid

    #157678

    Purpose

    C-terminal tagging vector

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    8His-Flag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. PERK 3: PERK.dC.9E10.pCDNA

    Plasmid

    #21816

    Depositor

    David Ron

    Article

    Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

    Insert

    PERK (C-term deleted) (Eif2ak3 Mouse)
    

    Use

    Tags

    Myc (9E10)

    Expression

    Mammalian

    Mutation

    amino-acids 582-1081 deleted

    Promoter

    Available since

    July 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO APEX2-eIF4E1

    Plasmid

    #129644

    Purpose

    Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

    Depositor

    Nicholas Ingolia

    Article

    Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

    Insert

    EIF4E1 (EIF4E Human)
    

    Use

    Tags

    APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-CD9-(TEV)-tTA

    Plasmid

    #162596

    Purpose

    human CD9-TEV cleavage site-tTA

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD9 (CD9 Human)
    

    Use

    Tags

    TEV protease cleavage site and tTA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-MTS-WT-c-Src-FLAG

    Plasmid

    #44652

    Depositor

    Yoshimi Homma

    Article

    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

    Insert

    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    

    Use

    Tags

    FLAG tag and mitochondria-targeting sequence (res…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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