We narrowed to 34,114 results for: IND

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pET151-GFP-PP-RPN2 (918-932)

Plasmid

#73748

Purpose

Expression of GFP-PP-RPN2 (918-932) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 918-932 of RPN2

Promoter

T7

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pTXB1-xlH2B(1-114)_K114A

Plasmid

#118217

Purpose

Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.

Depositor

Cynthia Wolberger

Article

Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

Insert

xlH2B(1-114)

Tags

Mxe-GyrA intein fused to chitin binding domain

Expression

Bacterial

Mutation

Lysine 114 to alanine

Promoter

T7

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_SIK3_T142Q

Plasmid

#108110

Purpose

Lentiviral expression plasmid of human SIK3 cDNA (gatekeeper mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

change Threonine 142 to Glutamine

Promoter

EFS promoter

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pETNKI-his-3C-LIC-Amp

Plasmid

#108704

Purpose

Expression of a His-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

His-3C protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_huMcl-1.2

Plasmid

#85531

Purpose

Inducible expression of guide RNA (huMcl-1.2) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

hu Mcl-1.2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1t

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-Claspin-224N

Plasmid

#31634

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

Insert

Claspin (CLSPN Human)

Tags

GST

Expression

Bacterial

Mutation

1st 224 amino acids of claspin

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
p252 pPr-CREM/CMV-STOP-luc

Plasmid

#8406

Purpose

Expression of modified Cre from androgen-inducible probasin promoter and LOX-dependent conditional reporter cassette.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CREM/lox/luciferase

Use

Cre/Lox

Expression

Mammalian

Mutation

modified Cre

Promoter

Probasin promoter, CMV

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pCELF PAI-RBP v1

Plasmid

#45506

Depositor

Thomas Gelehrter

Article

Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

Insert

PAI-RBP v1 (SERBP1 Rat, Human)

Tags

GST tag

Expression

Mammalian

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
pCELF PAI-RBP v2

Plasmid

#45505

Depositor

Thomas Gelehrter

Article

Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

Insert

PAI-RBP v2 (SERBP1 Rat, Human)

Tags

GST tag

Expression

Mammalian

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
PatoG-OG241

Plasmid

#72839

Purpose

Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoG

Expression

Bacterial

Promoter

PatoG

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pETDUET1-hUCH37 (ISF1)-NFRKB(39-156)

Plasmid

#61943

Purpose

Co-expression of hUCH37 (ISF1) and hNFRKB (39-156) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Inserts

hUCH37 (ISF1) (UCHL5 Human)
hNFRKB (1-156)

Tags

6x HIS

Expression

Bacterial

Mutation

Residues 2-39 deleted resulting in construct star…

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pSR04

Plasmid

#69151

Purpose

read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as gene of interest for integration on cxtTi10816, Mos Chr IV

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
TagBFP
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

eef-1A.1 (eft-3) and rps-27

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pTRC-APX(W41F) dimeric

Plasmid

#58499

Purpose

Bacterial expression of APX W41F inducible by IPTG

Depositor

Alice Ting

Article

Dwyer et al Proc Natl Acad Sci U S A. 2014 May 20;11

Insert

pea APX W41F (dimeric)

Tags

His6

Expression

Bacterial

Mutation

W41F

Promoter

trc

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-Claspin-BPI

Plasmid

#31633

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

Insert

Claspin (CLSPN Human)

Tags

GST

Expression

Bacterial

Mutation

aa 273-492 of claspin

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pEF Itk P158A P159A myc

Plasmid

#20708

Depositor

Leslie Berg

Article

Bunnell et al J Biol Chem. 2000 Jan 21. 275(3):2219-30

Insert

Itk PR (Itk Mouse)

Tags

myc

Expression

Mammalian

Mutation

P158A P159A. Inactivates the proline-rich region.

Available Since

March 17, 2009

Availability

Academic Institutions and Nonprofits only
pAAVS1-P-6XPyltRNA-dCas9K1151TAG-IRESPylRS

Plasmid

#248695

Purpose

Expression dead CRISPR/Cas9 with unnatural amino acid incorporated at K1151 residue

Depositor

Shuibing Chen

Article

Zhu et al Proc Natl Acad Sci U S A. 2025 Oct 7;122

Insert

dead CRISPR/Cas9, MbPylRS

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Split PS Intein TetR-vp16

Plasmid

#242023

Purpose

Blue-light-induced reconstitution of the tTA transactivator via split PS Intein-mediated fusion of TetR and vp16.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

Split PS Intein TetR-vp16

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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VEE-mScarlet3-IRES-EGFP-IRES-E3

Plasmid

#242408

Purpose

Self-amplifying RNA (saRNA) E3 construct; expresses the vaccinia virus E3 protein, which binds and sequesters dsRNA broadly inhibiting dsRNA-sensing pathways

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

mScarlet3
IRES-EGFP
IRES-E3

Use

Self-amplifying rna

Expression

Mammalian

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-scFv-GCN4-sfGFP-IRESv4-HaloTag-tdMCP

Plasmid

#241137

Purpose

Expresses Anti-GCN4 scFv-sfGFP and HaloTag-tdMCP to track mature and nascent SunTag-tagged proteins and MS2 tagged mRNA

Depositor

Tim Stasevich

Article

Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

Inserts

Anti-GCN4 scFv
tdMCP

Tags

HaloTag and sfGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET45 His-proSAAS (27 kDa)

Plasmid

#231637

Purpose

Bacterial expression of full-length mouse proSAAS chaperone protein

Depositor

Iris Lindberg

Article

Hoshino et al J Neurochem. 2014 Feb;128(3):419-30. doi

Insert

Pcsk1N (Pcsk1n Mouse)

Tags

6x His

Expression

Bacterial

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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