We narrowed to 89,560 results for: Mal

1. pcDNA6.2-YFP-CWT

   Plasmid

   #176689

   Purpose

   Fluorescent reporter for phagosomal escape of Staphylococcus aureus

   Depositor

   Martin Fraunholz

   Article

   Blattner et al PLoS Pathog. 2016 Sep 15;12(9):e1005857.

   Insert

   cell wall-targeting domain of Lysostaphin
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   deletion of protase domain of Lysostaphin

   Promoter

   CMV

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-HF-BE3

   Plasmid

   #87439

   Purpose

   Expresses HF-BE3 with C-terminal NLS in mammalian cells

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

   Insert

   HF-BE3 (Apobec1 Rat, Human, Bacillus phage PBS2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mammalian codon-optimized Cas9-HF

   Promoter

   CMV

   Available Since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLD-puro-Cn-VA-NHEJ1-WT

   Plasmid

   #141339

   Purpose

   Lentiviral vector for expression of human NHEJ1-WT in mammalian cells

   Depositor

   Mohan Babu

   Article

   Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

   Insert

   NHEJ1 (NHEJ1 Human)
   

   Use

   Lentiviral

   Tags

   VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. TAPBPL pmRFP-N2 (721)

   Plasmid

   #62000

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   TAPBPL (TAPBPL Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

5. Circular 200,100 (GAPDH)

   Plasmid

   #170120

   Purpose

   AAV vector carrying a guide RNA targeting the human GAPDH mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA (GAPDH Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-myc-PDGFRalpha-V242K

   Plasmid

   #136436

   Purpose

   Mammalian expression plasmid for c-myc-tagged PDGFRA (V242K mutant)

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   PDGFRalpha (PDGFRA Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   V242K (disrupts PDGF binding, but still binds HCM…

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDisplay-DNER

   Plasmid

   #51053

   Purpose

   expression of HA-DNER in mammalian cells

   Depositor

   Mineko Kengaku

   Article

   Eiraku et al J Biol Chem. 2002 Jul 12;277(28):25400-7

   Insert

   DNER (Dner Mouse)
   

   Use

   Tags

   HA and signal peptide

   Expression

   Mammalian

   Mutation

   without endogenous signal peptide (1-221bp)

   Promoter

   CMV

   Available Since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

8. AAV-hSyn-Ribo-jGCaMP8m

   Plasmid

   #167574

   Purpose

   DEPRECATED: Please see Addgene item #169248 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Has Service

   AAV1

   Insert

   Ribo-GCaMP8m
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode

   Plasmid

   #170220

   Purpose

   Barcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CDK1 (CDK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   T14A, Y15F

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG106 Lyn11-FAST

    Plasmid

    #130721

    Purpose

    Expresses FAST (also called YFAST) fused to Lyn11 sequence in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    lyn11-FAST
    

    Use

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX-HA-birA*-K-Ras(G12D)-IRES-Puro

    Plasmid

    #120562

    Purpose

    Expresses HA-birA*-K-Ras G12D fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    KRAS4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    CMV

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-PHFF

    Plasmid

    #100280

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    

    Use

    Tags

    FLAG, T7 and Mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    All genes are codon-optimized for expression in h…

    Promoter

    CAG promoter

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. EF1a_KLF4_P2A_Hygro_Barcode

    Plasmid

    #120455

    Purpose

    Barcoded lentiviral vector to express KLF4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG156 Mito-FAST

    Plasmid

    #130723

    Purpose

    Expresses FAST (also called YFAST) fused to a mitochondria targeting sequence (mito) in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    Mito-FAST
    

    Use

    Tags

    Myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-sPDGFRalpha-Fc

    Plasmid

    #136438

    Purpose

    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    PDGFRalpha (PDGFRA Human)
    

    Use

    Tags

    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…

    Expression

    Mammalian

    Mutation

    Extracellular domain of PDGFR-alpha (a.a. 1-524)

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-myc-S (Wuhan variant)

    Plasmid

    #169846

    Purpose

    Mammalian expression plasmid for S of SARS-CoV-2 (Wuhan variant) with N-terminal c-myc tag

    Depositor

    Erik Procko

    Article

    Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

    Insert

    Protein S
    

    Use

    Tags

    HA leader and c-myc tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG109 H2B-FAST

    Plasmid

    #130722

    Purpose

    Expresses FAST (also called YFAST) fused to zebrafish histone H2B in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    H2B-FAST
    

    Use

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTBL210

    Plasmid

    #126451

    Purpose

    Expresses Cas9-XTEN-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-XTEN-TdT (DNTT Human, Streptococcus pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-TagRFP658-P2A-GFP

    Plasmid

    #178971

    Purpose

    Expresses TagRFP658 and GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    TagRFP658-P2A-GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas9-NLS-gs10-cLYN268

    Plasmid

    #188259

    Purpose

    Plasmid ensures constitutive expression of the C-terminal fragment of split LYN kinase domain (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    cLYN268 (LYN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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