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pHR_Gal4UAS_Blinatumomab_PGK_mCherry

Plasmid

#85437

Purpose

[Edit] Lentiviral vector for Gal4 inducible Blinatumomab and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Blinatumomab

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only

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cisRAJ31-GFP-LAA

Plasmid

#69928

Purpose

Expresses cisRAJ31-GFP-LAA, a cis-repressed mRNA regulated by RAJ31.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

PllacO1-cisRAJ31-GFP-LAA

Use

Synthetic Biology

Expression

Bacterial

Promoter

PlLacO1

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD_C0000

Plasmid

#91081

Purpose

Module C, Promoter: none, Gene: none (BaeI site for GT donor cloning), Terminator: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Unspecified

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ control UTRs

Plasmid

#67929

Purpose

Translational reporter - control UTRs

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

sfGFP-NLS-P2A-NLS-mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-ZapCY2

Plasmid

#36320

Depositor

Amy Palmer

Article

Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

Insert

ZapCY2 high affinity Zn(II) sensor

Expression

Mammalian

Mutation

see comment

Promoter

CMV

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only
pMBP-FnCas12a

Plasmid

#113432

Purpose

Expresses 10xHis-MBP fused to FnCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

FnCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pTRANS_220d

Plasmid

#91114

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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mRNA-CleanCap-NanoLuc

Plasmid

#188247

Purpose

Template plasmid to transcribe mRNA encoding NanoLuc

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

NanoLuc

Use

Luciferase

Expression

Mammalian

Promoter

T7

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Endosomes-14

Plasmid

#58014

Purpose

Localization: Endosomes, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Endosomes (RHOB Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCAG-AKAP-mtagBFP2-UBC6

Plasmid

#105007

Purpose

Mammalian Expression of mtagBFP2

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

mtagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pFH3.2_mTagBFP2-MS2x12

Plasmid

#205558

Purpose

Express mTagBFP2-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mTagBFP2

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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prCAG-BoxB

Plasmid

#191122

Purpose

Transcription of 47xCAG and 12xBoxB repeats from a tetracycline-inducible promoter

Depositor

Ariel Lindner

Article

Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

Inserts

rCAG
BoxB

Use

Synthetic Biology

Promoter

tetracycline-inducible promoter

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pKD238-1a

Plasmid

#90958

Purpose

Expresses S. baltica TtrS constitutively

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

Tetrathionate sensor kinase

Expression

Bacterial

Promoter

Bba_J23109

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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SGL40C.EFS.dTomato

Plasmid

#89395

Purpose

Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with dTomato expression (EFS Promoter)

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMIN-VS1

Plasmid

#197783

Purpose

Level 2 destination vector: pMIN-VS1 backbone

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pY3

Plasmid

#50606

Purpose

Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5

Depositor

Jay Keasling

Article

Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

Inserts

tyrB
tyrA
aroC
aroA
aroL

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Nov. 14, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-Camk2-NES-Twitch-GR

Plasmid

#173044

Purpose

TnC-based calcium sensor with green-red FRET pair ratiometric readout

Depositor

Yiyang Gong

Article

Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

Has Service

AAV2

Insert

NES-Twitch-GR

Use

AAV

Expression

Mammalian

Promoter

CamK2

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-TID-HA3-HIS3MX6

Plasmid

#227981

Purpose

To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDD104 (Peft-3::Cre)

Plasmid

#47551

Purpose

Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

Depositor

Bob Goldstein

Article

Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

Insert

Cre Recombinase

Use

Cre/Lox

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pET22b RNase A

Plasmid

#58903

Purpose

Bacterial expression of bovine pancreatic ribonuclease A

Depositor

Ronald Raines

Article

delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

Insert

RNase A (RNASE1 Bovine)

Tags

pelB sequence

Expression

Bacterial

Mutation

See Depositor Comments below

Promoter

T7

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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