We narrowed to 63,812 results for: cloning
-
Plasmid#8838DepositorPublicationTypeEmpty backboneTagsP. furiosus MBPExpressionBacterialAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by
-
pAAVone-AAV2-CMV-eGFP
Plasmid#230930PurposepAAVone plasmid is used to package AAV-CMV-eGFP with an AAV2 serotype, utilizing the AAVone single-plasmid AAV production system.DepositorInserteGFP
UseAAVPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNF02-mLychee
Plasmid#219400PurposeConstitutive expression of mLychee in E. coliDepositorInsertmLychee
ExpressionBacterialPromoterproDpAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-C1-Nir1-LNS2
Plasmid#220083PurposeHigh affinity PA biosensor with green fluorophore (also known as NeonGreen-PILS-Nir1)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPITPNM3 (PITPNM3 Human)
TagsNeonGreen-GGSGGMExpressionMammalianMutationamino acids 613-897PromoterCMVAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)
Plasmid#214885PurposeLentiviral vector encoding TMP-regulatable RfxCas13d-DD fusionDepositorInsertEF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEF1aAvailable sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cys-60nmSAH-SPYcatcher
Plasmid#214840PurposeThis plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-60nmSAH-SPYcatcher
TagsHis-TEV-SUMO-ExpressionBacterialPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Mitochondrial cpGFP
Plasmid#215711PurposeExpresses cpGFP in the mitochondriaDepositorInsertcpGFP
UseLentiviralTagsFLAG, HA, and mitochondrial localization sequence…ExpressionMammalianMutationcircularly permuted GFPPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Nuclear cpGFP
Plasmid#215707PurposeExpresses cpGFP in the nucleusDepositorInsertcpGFP
UseLentiviralTagsFLAG, HA, and NLSExpressionMammalianMutationcircularly permuted GFPPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-q ONE-GO
Plasmid#189738PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralAvailable sinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEMS-II-PEST
Plasmid#204766PurposeGEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP-PEST-m6Alinker-DHFR
Mutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3
Plasmid#209730PurposeExpresses presynaptic low affinity ratiometric ATP sensorDepositorInsert(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3
UseLentiviralExpressionMammalianPromoterCamKIIAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLminP_Luc2P_RE6
Plasmid#90340PurposeSTAT5 - Stat5 gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterSTAT5Available sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-mCherry-MED1-IDR
Plasmid#194545PurposeBacterial Expression of 6xHis mCherry-MED1-IDR Fusion ProteinDepositorAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EGFP-p300
Plasmid#191764PurposeExpression of EGFP fused to core p300 histone acetyltransferaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFRB-EGFP-p300 core (EP300 Human)
TagsEGFP (N-terminal of p300 core) and FRB (N-termina…ExpressionBacterial and MammalianPromoterEF1alphaAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLIK-rABE-Flag
Plasmid#191383PurposeDoxycycline inducible expression of RNA A-to-I editor rABEDepositorInsertrABE-Flag
UseLentiviral and Synthetic BiologyTagsFlagExpressionMammalianPromotertet-inducible promoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-BFP
Plasmid#188776PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9-mTagBFP2
UseLentiviralTagsHA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusionPromoterSFFVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-TMEM106b-mApple
Plasmid#179385PurposeLentiviral vector for expression of TMEM106b fused mAppleDepositorInsertTMEM106b - mApple (TMEM106B Synthetic, Human)
UseLentiviralTagsmAppleExpressionMammalianPromoterEF1Available sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ERT2-iCre-ERT2
Plasmid#178059PurposeConditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.DepositorInsertERT2-iCre-ERT2
UseAAVExpressionMammalianPromoterHuman synapsin 1 (Syn) promoterAvailable sinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APPSwe-T2A-mCherry
Plasmid#190804PurposeExpress Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherryDepositorAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV9.452sub.LUNG1
Plasmid#184592Purposenon-standard AAV2 rep-AAV9.452sub.LUNG1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system for mouse lung transduction after intravenous injectionDepositorInsertSynthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
UseAAVExpressionMammalianMutation7 amino acid substitution between VP1 452 and VP1…Promoterp41Available sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only