We narrowed to 88,195 results for: MAL

1. pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro

   Plasmid

   #120565

   Purpose

   Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K, T35A

   Promoter

   CMV

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEX-uORF-HA-birA*-N-Ras(Q61K/Y40A)-IRES-Puro

   Plasmid

   #120567

   Purpose

   Expresses HA-birA*-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K, Y40A

   Promoter

   CMV

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX-uORF-HA-birA*-N-Ras(Q61K/E37A)-IRES-Puro

   Plasmid

   #120566

   Purpose

   Expresses HA-birA*-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K, E37A

   Promoter

   CMV

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMCh-1538

   Plasmid

   #82431

   Purpose

   Plasmid for expression of NHA-tagged human-Nematostella GW182 11W11A chimera in mammalian cells

   Depositor

   Marina Chekulaeva

   Article

   Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

   Insert

   hs-nvGW182 11W11A chimera
   

   Use

   Tags

   NHA

   Expression

   Mammalian

   Mutation

   aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …

   Promoter

   CMV

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. NKP9 pmRFP-N2 (719)

   Plasmid

   #61999

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   C17orf62 (CYBC1 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

6. NKP40 RFP (794)

   Plasmid

   #62006

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   C17orf32 (C17orf32 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   pgk

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pDest40 DHFR.dn-cHSF1

   Plasmid

   #134735

   Purpose

   Mammalian expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

   Insert

   DHFR.dn-cHSF1 (HSF1 Human)
   

   Use

   Tags

   DHFR

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 186-202, 379−529

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMasterK

   Plasmid

   #165081

   Purpose

   Episomal expression of reprogramming factors.

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

   Inserts

   OCT4 (POU5F1 Human)
   SOX2 (SOX2 Human)
   KLF4 (KLF4 Human)
   MYC (MYC Human)
   LRH1 (NR5A2 Human)
   NANOG (NANOG Human)
   lin28 (LIN28A Human)
   EGFP
   HSV TK
   KDM4D (KDM4D Callithrix jacchus)
   GLIS1 (GLIS1 Human)
   

   Use

   Episomal

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAG-Cas9-T2A-EGFP-ires-puro

   Plasmid

   #78311

   Purpose

   Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

   Depositor

   Timo Otonkoski

   Article

   Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

   Insert

   WT SpCas9-T2A-EGFP-ires-puromycin resistance
   

   Use

   CRISPR

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti-SpCas9 neo

    Plasmid

    #104996

    Purpose

    This lentiviral construct delivers hSpCas9 and G418 resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-SpCas9 hygro

    Plasmid

    #104995

    Purpose

    This lentiviral construct delivers hSpCas9 and hygromycin resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. lenti-SpCas9 blast

    Plasmid

    #104997

    Purpose

    This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-hERalpha

    Plasmid

    #101141

    Purpose

    Mammalian expression of full-length human estrogen receptor-alpha

    Depositor

    Elizabeth Wilson

    Article

    Mao et al J Biol Chem. 2008 May 9;283(19):12819-30

    Insert

    estrogen receptor-alpha (ESR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti-SpCas9 puro

    Plasmid

    #104994

    Purpose

    This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PE7_AAVS1_knockin_HDR_donor

    Plasmid

    #223001

    Purpose

    HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Hdr donor

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. LV_EF1a_FLAG-Ikkbeta(S177E,S181E)-P2A-Hygro_Barcode

    Plasmid

    #170226

    Purpose

    Barcoded lentiviral vector to express Ikkbeta (S177E, S181E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    IKBKB (IKBKB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    S177E, S181E

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. HDM_SARS2_Spike_del21_D614G

    Plasmid

    #158762

    Purpose

    Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614

    Depositor

    Jesse Bloom

    Article

    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

    Insert

    SARS2-Spike_del21_D614G (S Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted amino acids 1257-1278, changed aspartic a…

    Promoter

    CMV

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. LV_EF1a_Ikkalpha(S176E,S180E)-P2A-Hygro_Barcode

    Plasmid

    #170236

    Purpose

    Barcoded lentiviral vector to express Ikkalpha (S176E, S180E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CHUK (CHUK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    S176E, S180E

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-PHFF-sh-hBVRA

    Plasmid

    #100285

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Inserts

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for human BVRA
    

    Use

    Tags

    FLAG, T7 and Mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    All genes are codon-optimized for expression in h…

    Promoter

    CAG promoter and H1 promoter

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode

    Plasmid

    #170237

    Purpose

    Barcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    KRAS (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G12V

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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