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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 4441 - 4460 of 6562 results

  10. SauCas9 PE2*

    Plasmid
    #169851
    Purpose
    Mammalian Expression, sauCas9 based prime editor
    Depositor
    Scot Wolfe
    Publication
    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaCas9-N580A-M-MLV
    Tags
    bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    June 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    169851-plasmid-map-sequence-id-332821

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  11. pMB67

    Plasmid
    #47947
    Purpose
    Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter
    Depositor
    Mike Boxem
    Publication
    Waaijers et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and egl-13 NLS
    Expression
    Worm
    Promoter
    hsp-16.48
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    47947-plasmid-map-sequence-id-68808

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  13. pSL3718 (pQCascade_Pse)

    Plasmid
    #200899
    Purpose
    Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.
    Depositor
    Samuel H. Sternberg
    Publication
    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PseCas7, PseCas8, PseCas6, PseTniQ
    Tags
    NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)
    Expression
    Mammalian
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200899-plasmid-map-sequence-id-393105

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  15. SauKKHCas9 PE2*

    Plasmid
    #169852
    Purpose
    Mammalian Expression, sauCas9-KKH based prime editor
    Depositor
    Scot Wolfe
    Publication
    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaCas9KKH-N580A-M-MLV
    Tags
    bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169852-plasmid-map-sequence-id-337167

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  16. px459 VRER

    Plasmid
    #101716
    Purpose
    sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VRER
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VRER (D1135V, G1218R, R1335E and T1337R)
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    101716-plasmid-map-sequence-id-196973

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  18. Cas9-sgRNA-A+B

    Plasmid
    #149370
    Purpose
    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites
    Depositor
    Tarek Abbas
    Publication
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CBh, PGK, and U6
    Available since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    149370-plasmid-map-sequence-id-315474

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  20. TK4_SH4-1_targeting

    Plasmid
    #254452
    Purpose
    TK4 targeting vector containing left and right homology arms for the SH4-1 locus
    Depositor
    Marius Wernig
    Publication
    Uenaka et al Cell Stem Cell. 2026 Feb 13:S1934-5909(2
    Inserts
    mycNLS-mScarlet-T2A-mNeonGreen-PEST
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Piggybac
    Tags
    PEST, T2A-mycNLS-mTagBFP2, and c-myc-NLS
    Expression
    Mammalian
    Promoter
    CAG and EF1a
    Available since
    April 24, 2026
    Availability
    Academic Institutions and Nonprofits only
    254452-plasmid-map-sequence-id-507707

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Showing: 4441 - 4460 of 6562 results