We narrowed to 49,235 results for: LIS

1. pMH-SFB-DYRK1A

   Plasmid

   #101770

   Purpose

   Mammalian expression of DYRK1A fusion protein

   Depositor

   Michael Huen

   Article

   Li et al Nucleic Acids Res. 2024 Jul 5:gkae574. d

   Insert

   DYRK1A (DYRK1A Human)
   

   Use

   Tags

   S peptide-flag-streptavidin binding peptide (SFB)

   Expression

   Mammalian

   Mutation

   siRNA-resistant allele

   Promoter

   Available since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 7Gli:GFP

   Plasmid

   #110494

   Purpose

   reporter of Hedgehog signaling activity

   Depositor

   Michael Lewis

   Article

   Li et al PLoS Genet. 2016 May 19;12(5):e1006054.

   Insert

   7 repeats of the Gli binding site GAACACCCA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   7 repeats of Gli biding site

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX SH3(2)-4R

   Plasmid

   #112090

   Purpose

   Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   SH3(2)-4R (NCK1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HER2 CA (V659E)

   Plasmid

   #16259

   Depositor

   Mien-Chie Hung

   Article

   Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

   Insert

   HER2 (ERBB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Constitutively active: V659E

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSH-EFIRES-P-Seipin-miniIAA7-mScarlet-I

   Plasmid

   #129721

   Purpose

   AAVS1 safe harbor targeting vector expressing miniIAA7-mScarlet-I tagged Seipin

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   Seipin (BSCL2 Human)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   miniIAA7-mScarlet-I

   Expression

   Mammalian

   Mutation

   silent mutations

   Promoter

   EF1a

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNICKclos1.0

   Plasmid

   #73639

   Purpose

   Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

   Depositor

   Sheng Yang

   Article

   Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

   Inserts

   Cas9 nickase
   sGRNA to pyrE
   

   Use

   E.coli-clostridium shuttle vector

   Tags

   Expression

   Mutation

   D10A

   Promoter

   j23119 and ptb

   Available since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE

   Plasmid

   #111393

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   mCAR (Cxadr Mouse)
   GCaMP6f
   

   Use

   AAV

   Tags

   6xHis, Myc, T7, and Xpress

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX SH3(2)-3R

   Plasmid

   #112091

   Purpose

   Bacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   SH3(2)-3R (NCK1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSH-EFIRES-B-AtAFB2-mCherry

   Plasmid

   #129718

   Purpose

   AAVS1 safe harbor targeting vector with blasticidin resistance gene expressing AtAFB2-mCherry

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   AFB2 (AFB2 Mustard Weed)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   EF1a

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 1313_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-SACas9-HA-OLLAS-spA

    Plasmid

    #109314

    Purpose

    Plasmid for liver-specific expression of AAV SaCas9 with a BbsI cloning site for a gRNA

    Depositor

    William Lagor

    Article

    Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEXPqcxip-hCCDC47-FLAG

    Plasmid

    #159141

    Purpose

    Hu CCDC47 -flag mammalian expression Gateway vector.

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    coiled-coil domain containing 47 (CCDC47 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (cytomegalovirus) enhancer and the MSV (mouse…

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNICKclos2.0

    Plasmid

    #73228

    Purpose

    Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    Cas9 nickase
    sgRNA to xylR
    

    Use

    E.coli - clostridium shuttle vector

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Pj23119 and Pthl

    Available since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 1518_pAAV-U6-SA-eGFP-gRNA-HLP-SACas9-HA-OLLAS-spA

    Plasmid

    #109316

    Purpose

    Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against eGFP

    Depositor

    William Lagor

    Article

    Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

    Insert

    eGFP gRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Rescue Septin 11 mRNA

    Plasmid

    #122022

    Purpose

    Rescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 (Sept11 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations are in 5 consecutive codons (AUU…

    Promoter

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-hSyn-DIO {hCAR}off-{ChETA}on-WPRE

    Plasmid

    #111388

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    hCAR (CXADR Human)
    ChETA
    

    Use

    AAV

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-dCpf1-eBE

    Plasmid

    #107688

    Purpose

    Expresses dLbCpf1-eBE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-eBE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dLbCpf1(D832A/E925A/D1148A)

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mMib1-FLAG

    Plasmid

    #37116

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    Mib1(Mus musculus mindbomb homolog 1 (Drosophila) (Mib1)) (Mib1 Mouse)
    

    Use

    Tags

    3 x FLAG

    Expression

    Mammalian

    Mutation

    ACC Kozak sequence added before ATG annotated.

    Promoter

    CMV

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-hSyn-DIO {mCAR}off-{DTR-GFP}on-WPRE

    Plasmid

    #111395

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    mCAR (Cxadr Mouse)
    DTR-EGFP
    

    Use

    AAV

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLX317-hcRED

    Plasmid

    #115440

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    hcRED
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-mPCSK3 H194L

    Plasmid

    #122689

    Purpose

    Expresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Histidine 194 to Leucine

    Promoter

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge