We narrowed to 12,019 results for: pcdna

1. pcDNA3.1(+)-DnaJB1-PKAcat-mCherry

   Plasmid

   #181850

   Purpose

   mCherry-tagged DnaJB1-PKA catalytic subunit fusion; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5 FRT-TO-EGFP-UPF1-WT

   Plasmid

   #136000

   Purpose

   DOX-inducible WT UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   UPF1
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO CBX1-APEX2

   Plasmid

   #129642

   Purpose

   Express heterochromatin-localized CBX1-APEX2 fusion in mammalian cells

   Depositor

   Nicholas Ingolia

   Article

   Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

   Insert

   CBX1 (CBX1 Human)
   

   Use

   Tags

   1xFLAG and APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1/Puro-CAG-JEDI-2P-Kv

   Plasmid

   #179462

   Purpose

   Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Insert

   JEDI-2P-Kv
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT-TO-Nprot-HF_GC3opt

   Plasmid

   #157732

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nprot-HF_GC3opt (N SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available Since

   Aug. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3.1/myc-his Ndel1

   Plasmid

   #12572

   Depositor

   Li-Huei Tsai

   Article

   Shu et al Neuron. 2004 Oct 14. 44(2):263-77.

   Insert

   Ndel1 (NDEL1 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1-mouse cdk6 D163N

   Plasmid

   #75173

   Purpose

   Mammalian expression of mouse cdk6 with D163N

   Depositor

   Martine Roussel

   Insert

   cyclin dependent kinase 6 (Cdk6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D163N

   Promoter

   CMV

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA5/FRT/TO APEX2-eIF4E1

   Plasmid

   #129644

   Purpose

   Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

   Depositor

   Nicholas Ingolia

   Article

   Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

   Insert

   EIF4E1 (EIF4E Human)
   

   Use

   Tags

   APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4TO-24xGCN4_v1-P2A-sfGFP

   Plasmid

   #61057

   Purpose

   sfGFP and the 24xGCN4_v1 peptide array are expressed from the same transcript, separated by a P2A site

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   24xGCN4_v1 and sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA5-FRT-TO-AID-EGFP

    Plasmid

    #80076

    Purpose

    Mammalian expression vector for C-terminal AID-EGFP tagging

    Depositor

    Andrew Holland

    Insert

    AID-EGFP
    

    Use

    Tags

    AID and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3puro-mGFP-FMR1iso1-G266E

    Plasmid

    #225468

    Purpose

    Express mEGFP-fusion protein of FMR1 isoform 1 (G266E)

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FMR1 isoform 1 (FMR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    G266E

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3puro-mGFP-FMR1iso1-I304N

    Plasmid

    #225463

    Purpose

    Express mEGFP-fusion protein of FMR1 isoform 1 (I304N)

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FMR1 isoform 1 (FMR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    I304N

    Promoter

    CMV

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Y951F-VEGFR2-mCherry

    Plasmid

    #139607

    Purpose

    Expresses a mutant (Y951F) VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNAintron-CHIKV-nsp3-c3xFLAG

    Plasmid

    #215696

    Purpose

    Produces the Chikungunya virus nonstructural protein 3 with a c-terminal 3xFLAG tag

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV nsp3
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA-LBR-mRFP1-polyA

    Plasmid

    #59749

    Purpose

    Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    LBR-mRFP1
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    aa 1-249 of LBR

    Promoter

    CMV and T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)-HLA-Flag-natT1R2

    Plasmid

    #113947

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from HLA class I …

    Expression

    Mammalian

    Mutation

    residues 22-839

    Promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA5-TEX264-deltaLRR-cSSH

    Plasmid

    #220210

    Purpose

    Mammalian expression of TEX264, 2-25 residues (LRR) truncated, C-terminal twin-Strep_HA tag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Strep-strep-HA

    Expression

    Mammalian

    Mutation

    c.4-75del / p.2-24del

    Promoter

    CMV/TetO

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-hRIPK1-Flag-delN

    Plasmid

    #112492

    Purpose

    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of N terminus (AA1-274 deleted)

    Promoter

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.2/V5 mmu-mir-133a-1

    Plasmid

    #26326

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-133a-1 (Mir133a-1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA5-FRT-TO-EGFP-AID

    Plasmid

    #80075

    Purpose

    Mammalian expression vector for N-terminal EGFP-AID tagging

    Depositor

    Andrew Holland

    Insert

    EGFP-AID
    

    Use

    Tags

    AID and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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