We narrowed to 17,914 results for: REV

1. YIp128-ADH-NUbo-E3(48)-mCherry-VSV

   Plasmid

   #212748

   Purpose

   Constitutive expression of NUbo-E3(48)-mCherry-VSV in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(48)
   

   Use

   Integrative vector

   Tags

   NUbo and mCherry-VSV

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. YIp128-ADH-NUbo-E3(48)-NLS-VSV

   Plasmid

   #212745

   Purpose

   Constitutive expression of NUbo-E3(48)-NLS-VSV in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(48)
   

   Use

   Integrative vector

   Tags

   NLS-VSV and NUbo

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. YIp211-ADH-myc-E3(1)-CUbo-mCherry-VSV

   Plasmid

   #212740

   Purpose

   Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(1)
   

   Use

   Integrative vector

   Tags

   CUbo-mCherry-VSV and myc

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. YIp128-ADH-myc-E3(1)-CUbo-mCherry-VSV

   Plasmid

   #212739

   Purpose

   Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(1)
   

   Use

   Integrative vector

   Tags

   CUbo-mCherry-VSV and myc

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. YIp128-ADH-myc-E3(1)-CUbo-NLS-VSV

   Plasmid

   #212737

   Purpose

   Consitutive expression of myc-E3(1)-CUbo-NLS-VSV in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(1)
   

   Use

   Integrative vector

   Tags

   CUbo-NLS-VSV and myc

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. YIp211-ADH-myc-E3(1)-CUbo-VSV

   Plasmid

   #212736

   Purpose

   Consitutive expression of myc-E3(1)-CUbo-VSV in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(1)
   

   Use

   Integrative vector

   Tags

   CUbo-VSV and myc

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. YIp128-ADH-myc-E3(1)-CUbo-VSV

   Plasmid

   #212735

   Purpose

   Consitutive expression of myc-E3(1)-CUbo-VSV in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(1)
   

   Use

   Integrative vector

   Tags

   CUbo-VSV and myc

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. YIp128-ADH-CUbo-mCherry-VSV

   Plasmid

   #212734

   Purpose

   Consitutive expression of CUbo-mCherry-VSV in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   CUbo
   

   Use

   Integrative vector

   Tags

   mCherry-VSV

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YIp128-ADH-NUbo-mCherry-VSV

   Plasmid

   #212731

   Purpose

   Consitutive expression of NUbo-mCherry-VSV in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   NUbo
   

   Use

   Integrative vector

   Tags

   mCherry-VSV

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5-FRT-TO-NUbo-H2B-His8

    Plasmid

    #212810

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-H2B-His8 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    H2B
    

    Use

    Tags

    8xHis and NUbo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-FRT-TO-His6-NUbo-H2B-His8

    Plasmid

    #212811

    Purpose

    Constitutive or doxycycline-inducible expression of His6-NUbo-H2B-His8 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    H2B
    

    Use

    Tags

    6xHis-NUbo and 8xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5-FRT-TO-His8-H2B-CUbo

    Plasmid

    #212812

    Purpose

    Constitutive or doxycycline-inducible expression of His8-H2B-CUbo in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    H2B
    

    Use

    Tags

    8xHis and CUbo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5-FRT-TO-His8-H2B-CUbo(K48R)

    Plasmid

    #212813

    Purpose

    Constitutive or doxycycline-inducible expression of His8-H2B-CUbo(K48R) in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    H2B
    

    Use

    Tags

    8xHis and CUbo(K48R)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p663-UBC-miniTurbo-V5-MAFG

    Plasmid

    #207817

    Purpose

    N-terminal tagged MAFG for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    miniTurbo-V5-MAFG
    

    Use

    Lentiviral

    Tags

    miniTurbo-V5

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p663-UBC-miniTurbo-V5-ZNF746

    Plasmid

    #207819

    Purpose

    N-terminal tagged ZNF746 for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    miniTurbo-V5-ZNF746 (ZNF746 Human)
    

    Use

    Lentiviral

    Tags

    miniTurbo-V5

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. WT_IFL_G1A-T2A_R

    Plasmid

    #200183

    Purpose

    WT PA14 I-F Leader and G1A-T2A Repeat

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G1A-T2A in the I-F repeat

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-Cas9-NS-A

    Plasmid

    #207823

    Purpose

    Dual expression of Cas9 and non-specific sgRNA

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    non-specific sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    EF-1a; U6

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-Cas9-NS-B

    Plasmid

    #207824

    Purpose

    Dual expression of Cas9 and non-specific sgRNA

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    non-specific sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    EF-1a; U6

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCW57.1-ZNF746-C571A

    Plasmid

    #207826

    Purpose

    Dox-inducible expression of ZNF746-C571A

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    ZNF746-C571A (ZNF746 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    C571A

    Promoter

    TRE

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p667-UBC-ZNF746-V5-miniTurbo

    Plasmid

    #207820

    Purpose

    C-terminal tagged ZNF746 for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    ZNF746-V5-miniTurbo (ZNF746 Human)
    

    Use

    Lentiviral

    Tags

    V5-miniTurbo

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCR 2.1-TOPO Fzd6 N352A HA

    Plasmid

    #208365

    Purpose

    Extracellular HA-tagged Fzd6 N352A (mouse)

    Depositor

    Maureen Cetera, Danelle Devenport

    Article

    Cetera et al Development. 2023 Aug 25:dev.202078. doi

    Insert

    Fzd6 (Fzd6 Mouse)
    

    Use

    Cloning vector

    Tags

    HA

    Expression

    Mutation

    Asparagine 352 to Alanine

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCR 2.1-TOPO Fzd6 S354I HA

    Plasmid

    #208364

    Purpose

    Extracellular HA-tagged Fzd6 S354I (mouse)

    Depositor

    Maureen Cetera, Danelle Devenport

    Article

    Cetera et al Development. 2023 Aug 25:dev.202078. doi

    Insert

    Fzd6 (Fzd6 Mouse)
    

    Use

    Cloning vector

    Tags

    HA

    Expression

    Mutation

    Serine 354 to Isoleucine

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCR 2.1-TOPO Fzd6HA

    Plasmid

    #208363

    Purpose

    Extracellular HA-tagged Fzd6 (mouse)

    Depositor

    Maureen Cetera, Danelle Devenport

    Article

    Cetera et al Development. 2023 Aug 25:dev.202078. doi

    Insert

    Fzd6 (Fzd6 Mouse)
    

    Use

    Cloning vector

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET 15b+His SUMO Rab12 (Q101L)

    Plasmid

    #208371

    Purpose

    Bacterial expression of His-SUMO-tagged human Rab12 (Q101L)

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    His SUMO Rab12 (Q101L) (RAB12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q101L

    Promoter

    Available since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRH3175

    Plasmid

    #210177

    Purpose

    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1

    Depositor

    Randy Hampton

    Article

    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

    Insert

    YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1 (ARO7 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R33G,G141S

    Promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHD57 [Tol2-UAS:sypb-egfp-cry2-polyA]

    Plasmid

    #198381

    Purpose

    Expression of SYPB::CRY2olig(535) in neurons of Danio rerio

    Depositor

    Alexander Gottschalk

    Article

    Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

    Insert

    UAS:sypb-egfp-cry2
    

    Use

    Tol2

    Tags

    EGFP

    Expression

    Mutation

    D387A

    Promoter

    UAS

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ALIX(P801G)-mNeonGreen

    Plasmid

    #191192

    Purpose

    Full-length ALIX with P801G mutation in proline-rich domain; C-terminally tagged with mNeonGreen.

    Depositor

    Jin Zhang

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX (P801G) (PDCD6IP Synthetic, Human)
    

    Use

    Tags

    6xHIS and mNeonGreen

    Expression

    Mammalian

    Mutation

    Pro 801 mutated to Gly

    Promoter

    CMV

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV_8xTEAD_luc2

    Plasmid

    #173005

    Purpose

    firefly luciferase reporter vector with 8x clustered TEAD binding sites

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    8xTEAD-luc2
    

    Use

    AAV and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28 sf:em-blue-shift.1

    Plasmid

    #191928

    Purpose

    Encodes a GFP design, sf:em-blue-shift.1

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:em-blue-shift.1
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S Q69P S72A T108V Y145M T167V V224I

    Promoter

    T7

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKG-GW2-NUS1

    Plasmid

    #203169

    Purpose

    Yeast expression vector for yNUS1

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    NUS1 (NUS1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pKG-GW1-RER2

    Plasmid

    #203170

    Purpose

    Yeast expression vector for yeast RER2

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    RER2 (RER2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLVX-CALPAIN-7-mCherry, F98D

    Plasmid

    #180653

    Purpose

    Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. F98D mutation. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-53.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRNA resistant to: GCACCCAUACCUUUACAUU

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCA528 RPS6KL1 MIT, residues 20-140

    Plasmid

    #180623

    Purpose

    Bacterial expression for RPS6KL1 MIT domain, residues 20-140. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-32.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    RPS6KL1 (RPS6KL1 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 20-140

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGEX-THTAP-SNX15, residues 263-342

    Plasmid

    #180624

    Purpose

    Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-GST tag. Internal ID:WISP21-130.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SNX15 (SNX15 Human)
    

    Use

    Tags

    HIS-GST

    Expression

    Bacterial

    Mutation

    Residues 263-342

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCA528 SNX15 MIT residues 263-342

    Plasmid

    #180625

    Purpose

    Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-SUMO tag. Internal ID:WISP21-14

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SNX15 (SNX15 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 263-342

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCA528 SPASTIN , F124D, residues 108-200

    Plasmid

    #180629

    Purpose

    Bacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 108-200, F124D

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCA528 SPASTIN , L177D, residues 108-200

    Plasmid

    #180634

    Purpose

    Bacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 108-200, L177D

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCA528 USP8, residues 1-147

    Plasmid

    #180636

    Purpose

    Bacterial expression for USP8 MIT domain, residues 1-147. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-86.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    USP8 (USP8 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-147

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCA528 USP54, residues 731-878

    Plasmid

    #180637

    Purpose

    Bacterial expression for USP54 MIT domain, residues 731-878. Has N-terminal HIS-SUMO tag. Internal ID:WISP20-85

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    USP54 (USP54 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET16B VPS4A, residues 1-84

    Plasmid

    #180638

    Purpose

    Bacterial expression for VPS4A MIT domain residues 1-84. Has N-terminal HIS-TAG. Internal ID: WISP05-43

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    VPS4A (VPS4A Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Residues 1-84

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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41. pCA528 CALPAIN-7 residues 1-165, V18D

    Plasmid

    #180612

    Purpose

    Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has V18D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-26.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-165, V18D mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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42. pCA528 CALPAIN-7 residues 1-165, F98D

    Plasmid

    #180613

    Purpose

    Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has F98D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-27.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-165, F98D mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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43. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

    Plasmid

    #197426

    Purpose

    π-element knock-in into exon 5 of MAPRE1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. pMVQA

    Plasmid

    #197894

    Purpose

    pCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))

    Depositor

    Thomas Klimkait

    Article

    Otte et al Cell Rep Methods. 2023 May 23;3(6):10048

    Insert

    HIV-1 3' end (HXB2: 9045 till poly-A(10)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. pTP-PCR C3

    Plasmid

    #189620

    Purpose

    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

    Insert

    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Removed repeat region, 10-kbp deletion, and minor…

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. pGA-red-mini

    Plasmid

    #196338

    Purpose

    Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology; Golden gate assembly backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pGA-red-maxi

    Plasmid

    #196337

    Purpose

    Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology; Golden gate assembly backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. pGA-blue

    Plasmid

    #196336

    Purpose

    Easy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screening

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology; Golden gate assembly backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD

    Plasmid

    #199447

    Purpose

    Expession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signal

    Depositor

    Baohui Chen

    Article

    Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

    Insert

    dSaCas9-10xGCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C (UBC)

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pJR032

    Plasmid

    #198773

    Purpose

    Stabilized step function Opsins ChR2 variant

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::hChR2 (C128S, D156A)-EYFP::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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