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pLVX-M-Flag-BAP1-E31A

Plasmid

#125843

Purpose

express mutant BAP1-E31A

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

E31A

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1-S10T

Plasmid

#125842

Purpose

express mutant BAP1-S10T

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

S10T

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-HLA-G-COMP5AP-AviTag-9xHis

Plasmid

#157397

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

HLA-G (HLA-G Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SEMA3F-Fc(DAPA)-AviTag-6xHis

Plasmid

#156977

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA3F (SEMA3F Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-MICA-COMP5AP-AviTag-9xHis

Plasmid

#157289

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

MICA (MICA Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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DYRK2_HUMAN_D0

Plasmid

#79729

Purpose

This plasmid encodes the kinase domain of DYRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DYRK2 (DYRK2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pLVX-M-Flag-BAP1-L49V

Plasmid

#125844

Purpose

express mutant BAP1-L49V

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

L49V

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-2gamma

Plasmid

#124145

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-p18Ink4c-IRES-GFP

Plasmid

#52220

Purpose

retroviral bicistronic expression of p18Ink4c and GFP

Depositor

Martine Roussel

Article

Forget et al Cell Cycle. 2008 Dec;7(23):3737-46. Epub

Insert

Ink4c (Cdkn2c Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA5_FRB-pBirA-HA

Plasmid

#83415

Purpose

proximity-directed biotin labeling on the FRB domain of mTOR in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al ACS Cent Sci. 2016 Aug 24;2(8):506-16. d

Insert

FRB-pBirA-HA (MTOR Human, Synthetic)

Tags

HA and pBirA

Expression

Mammalian

Promoter

CMV-F

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pME mKate2-dynactin1-811

Plasmid

#105971

Purpose

entry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

dynactin1-811 (DCTN1 Human)

Tags

mKate2

Expression

Bacterial

Mutation

contains only amino acids 1-811

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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KLC1-Myc

Plasmid

#166962

Purpose

Expresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KLC1 (Klc1 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-VCAM1-COMP5AP-AviTag-9xHis

Plasmid

#157506

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

VCAM1 (VCAM1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1-F170C

Plasmid

#125848

Purpose

express mutant BAP1-F170C

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

F170C

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1-G109V

Plasmid

#125846

Purpose

express mutant BAP1-G109V

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

G109V

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-1alpha

Plasmid

#124139

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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sgCRY2

Plasmid

#197457

Purpose

Expresses sgRNA(CRY2 gene) in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCRY2-1-hU6-sgCRY2-2 (Cry2 Mouse)

Use

AAV

Tags

mCherry (K14E, N210D, E249G)

Promoter

U6, hSyn

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF0032

Plasmid

#141484

Purpose

Lentiviral vector for overexpressing the GSC2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GSC2 (GSC2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-TNFRSF6B-COMP5AP-AviTag-9xHis

Plasmid

#157277

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TNFRSF6B (TNFRSF6B Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC35D2

Plasmid

#132122

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35D2 (SLC35D2 Human)

Expression

Mammalian

Available Since

Nov. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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