We narrowed to 15,167 results for: Egf

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pEGFP-NMHC-IIA-S1916A

Plasmid

#101040

Purpose

Expresses GFP-MYH9 construct (human myosin IIA) carrying mutation of serine 1916 to alanine, driven by CMV promoter. Thus inhibits PKC phosphorylation of the tail. Also carries S1915A mutation.

Depositor

Tom Egelhoff

Article

Rai et al J Biol Chem. 2017 Feb 24;292(8):3099-311

Insert

Non-muscle myosin IIA heavy chain (MYH9 Human)

Tags

GFP

Expression

Mammalian

Mutation

S1916A, S1917A

Promoter

CMV

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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eGFP Y93H AC editing reporter

Plasmid

#198883

Purpose

Express eGFP fluorescence after Y93H correction and mCherry as transfection control

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Mutation

Y93H, Gly92(GGT-to-GGA)

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA4 (MSW#517)

Plasmid

#208070

Purpose

Expression of GFP-tagged hsRPA4 in human cells

Depositor

Marc Wold

Article

Haring et al Nucleic Acids Res. 2010 Jan;38(3):846-58

Insert

RPA4 (RPA4 Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-nl.EGFP-SypGFP

Plasmid

#172380

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized EGFP + SypEGFP

Use

Neurotropic virus

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A-APEX2-csChBP

Plasmid

#108877

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against mCherry (includes green cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2-csChBP

Expression

Mammalian

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP(C3)-Nup153-C (880-1475)

Plasmid

#64317

Purpose

GFP-tagged mammalian expression of C-terminal domain of Nup153

Depositor

Birthe Fahrenkrog

Article

Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

Insert

Nup153 (NUP153 Human)

Tags

GFP

Expression

Mammalian

Mutation

C-terminal subunit (880-1475)

Promoter

CMV

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-C3-PLK4 K41M-3xFLAG

Plasmid

#69838

Purpose

This plasmid encodes kinase dead PLK4 isoform 1 (K41M mutation) with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

K41M

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-ULK1(K46N)

Plasmid

#38197

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed Lysine 46 to Asparagine

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
psubCAG-WPRE-hVEGF-D-Strept

Plasmid

#119227

Purpose

Plasmid encodes Strep-tagged full-length (unprocessed) VEGF-D of human origin to be used for stimulation of lymphangiogenesis in mice in vivo.

Depositor

Kari Alitalo

Insert

VEGFD (VEGFD Human)

Use

AAV

Tags

Strept

Promoter

CAG

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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BE3-P2A-EGFP (pJUL977)

Plasmid

#123612

Purpose

CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3-P2A-EGFP

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A-APEX2-ChBP

Plasmid

#108879

Purpose

Expression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2-ChBP

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-cmlc2-Trpa1b-2A-EGFP

Plasmid

#106405

Purpose

For expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.

Depositor

Randall Peterson

Article

Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

Inserts

cmlc2 promoter
Trpa1b (trpa1b Zebrafish)

Use

Gateway

Tags

2A-EGFP

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HIV-Rev_O

Plasmid

#147113

Purpose

Mammalian Expression of HIV-Rev

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HIV-Rev

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTRE3G-EGFPC-uL10-(Neo)R

Plasmid

#158069

Purpose

eGFP and uL10 ribosomal protein under the TRE3G promoter, Neo resistance

Depositor

Adolfo Rivero-Muller

Article

Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

Insert

RPLP0 (RPLP0 Human)

Tags

eGFP

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/MSCV-EGFP

Plasmid

#82613

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g

Insert

3xFLAG-dCas9

Use

Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Mutation

human codon-optimized, D10A + H840A

Promoter

LTR

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_off-mEGFP_WRPE-SV40

Plasmid

#194692

Purpose

hSyn1 driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_off-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-PHOX2B-MUT

Plasmid

#194577

Purpose

Mammalian Expression of mEGFP-PHOX2B Mutant (S207Afs*102) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

PHOX2B (PHOX2B Human)

Tags

mEGFP

Expression

Mammalian

Mutation

S207Afs*102

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP Y93H TC editing reporter

Plasmid

#198882

Purpose

Express eGFP fluorescence after Y93H correction and mCherry as transfection control

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Mutation

Y93H

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo

Plasmid

#160458

Purpose

Homology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTR

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Inserts

PAX7 Left/5' Homology Arm (PAX7 Human)
PAX7 Right/3' Homology Arm (PAX7 Human)

Use

Insertion of a fluorescent reporter through homol…

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Y951F-VEGFR2-mCherry

Plasmid

#139607

Purpose

Expresses a mutant (Y951F) VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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