We narrowed to 17,148 results for: tes

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pCMV3Tag8li-hADARB2-3xFLAG

Plasmid

#165470

Purpose

Human ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Adenosine deaminase RNA specific B2 (inactive) (ADARB2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mScarletI-NLS-blast

Plasmid

#136260

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mScarletI-NLS

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Strep/HA-UBE2J1

Plasmid

#124669

Purpose

Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2J1 (UBE2J1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-hAtg12

Plasmid

#87869

Purpose

Express a EGFP-human Atg12/Apg12 in mammalian cells

Depositor

Eiki Kominami, Isei Tanida

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

ATG12 (ATG12 Human)

Expression

Mammalian

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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p2K7-bsd-UBI-tagRFP-KDEL

Plasmid

#114179

Purpose

Lentiviral vector for expression of tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

Depositor

Nicolas Demaurex

Article

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

tagRFP-KDEL

Use

Lentiviral

Tags

KDEL retention signal and signal peptide

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2-Sox9 T236A

Plasmid

#111455

Purpose

Transient expression of Flag-tagged human Sox9 with T236A mutation

Depositor

Sharon Pine

Article

Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

Insert

Sox9 (SOX9 Human)

Tags

Flag

Expression

Mammalian

Mutation

T236A mutation

Promoter

CMV

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-Ag43

Plasmid

#107743

Purpose

Expresses Ag43 under the control of an L-arabinose transcriptional regulator

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

Insert

Ag43 (flu )

Expression

Bacterial

Mutation

removed all pstI cut sites with silent mutations,…

Promoter

pBAD

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3 3'UTR reporter MUT 1.3 kb CD274 Hs 3'UTR Final

Plasmid

#107010

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING-342STOP

Plasmid

#102588

Purpose

Lentivector to express STING delta 342 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta 342

Promoter

SFFV

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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TUBG1

Plasmid

#87954

Purpose

expresses human gamma-tubulin with a GFP-tag on the N-terminal of the protein

Depositor

Maria Alvarado-Kristensson

Article

Rossello et al Heliyon. 2016 Sep 24;2(9):e00166. eColle

Insert

gamma-tubulin 1 (TUBG1 Human)

Tags

GFP

Expression

Mammalian

Mutation

whole protein, The C-terminal is a sh-gamma-tubul…

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221 UBR5 ∆UBR

Plasmid

#81063

Purpose

Gateway entry vector encoding full length UBR5 W1235L (∆UBR)

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)

Use

Cloning

Mutation

W1235L (bp g3704t)

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro-GFPi

Plasmid

#31848

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pSIREN-RetroQ-CDK6-shRNA

Plasmid

#25789

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

shRNA against human CDK6 (CDK6 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
pGL2-50bp

Plasmid

#16295

Depositor

Wafik El-Deiry

Article

Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

Insert

p53 promoter fragment (TP53 Human)

Use

Luciferase

Expression

Mammalian

Mutation

fragment -38 to +12 relative to first transcripti…

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pInducer10b-HA-KRAS G12V CO SR

Plasmid

#164928

Purpose

Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRASG12V sequence

Depositor

Ji Luo

Article

Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.

Insert

KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)

Use

Lentiviral

Tags

HA tag

Mutation

Glycine 12 changed to valine; 9 Valine codons are…

Promoter

Ubc promoter

Available Since

May 27, 2021

Availability

Academic Institutions and Nonprofits only

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pJKR-O-acuR

Plasmid

#62569

Purpose

Acrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

CFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pEx_MCS-attBtagRFPt

Plasmid

#48876

Purpose

PhiC31 exchange vector with multiple cloning site, exchange of EGFP in landing site with tagRFPt

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al Development. 2013 Sep 18.

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-3xFLAG-BioID2-MCS

Plasmid

#186811

Purpose

CAG promoter-driven expression of BioID2-fused protein (N terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Tags

3xFLAG-BioID2

Expression

Mammalian

Promoter

CAG promoter

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-hLC3

Plasmid

#87872

Purpose

Express a EGFP-human MAP1-LC3 in mammalian cells

Depositor

Eiki Kominami, Isei Tanida

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

MAP1LC3 (MAP1LC3B Human)

Expression

Mammalian

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221 UBR5 ∆HECT

Plasmid

#81065

Purpose

Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)

Use

Cloning

Mutation

C2768A (bp t8302g and g8303c)

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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