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pAW8_mCherry

Plasmid

#110238

Purpose

Cre/Lox C-terminal tagging construct encoding red fluorescent protein mCherry

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_13Myc

Plasmid

#110232

Purpose

Cre/Lox C-terminal tagging construct encoding 13 copies of the human c-myc (Myc) epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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spoT-

Plasmid

#100913

Purpose

deletes synpcc7942_1377 (spoT)

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

Kanamycin casette (in place of spoT gene) flanked by S. elongatus sequence

Use

Cloning vector

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAW32

Plasmid

#110225

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt1 promoter

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW33

Plasmid

#110226

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt41 promoter

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW34

Plasmid

#110227

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt81 promoter

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_yEGFP

Plasmid

#110229

Purpose

Cre/Lox C-terminal tagging construct encoding codon-optimized green fluorescent protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_1XFLAG

Plasmid

#110234

Purpose

Cre/Lox C-terminal tagging construct encoding single copy of the FLAG epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_Xho

Plasmid

#110223

Purpose

Cre-expression plasmid for cassette exchange but MCS modified compared to pAW8

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_TAP

Plasmid

#110240

Purpose

Cre/Lox C-terminal tagging construct encoding the Twin Affinity Purification protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHES944

Plasmid

#112642

Purpose

express SST2 from the GAL1 promoter

Depositor

Hana El-Samad

Article

Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

Insert

SST2

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_3HA

Plasmid

#110233

Purpose

Cre/Lox C-terminal tagging construct encoding 3 copies of the influenza virus hemagglutunin (HA) epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_yECitrine

Plasmid

#110231

Purpose

Cre/Lox C-terminal tagging construct encoding codon-optimized yellow fluorescent protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pNX3a-HA3

Plasmid

#78359

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA
kanMX6

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pEZ04

Plasmid

#81127

Purpose

pACSA_J23109_tetR PEZ1_gfp

Depositor

Brian Pfleger

Article

Zess et al Biotechnol Bioeng. 2016 Feb;113(2):424-3

Insert

GFP

Promoter

PEZ1

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pEZ12

Plasmid

#81138

Purpose

pACSA_J23113_tetR PEZ4_gfp

Depositor

Brian Pfleger

Article

Zess et al Biotechnol Bioeng. 2016 Feb;113(2):424-3

Insert

GFP

Promoter

PEZ4

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEZ14

Plasmid

#81140

Purpose

pACSA_J23113_tetR PEZ5_gfp

Depositor

Brian Pfleger

Article

Zess et al Biotechnol Bioeng. 2016 Feb;113(2):424-3

Insert

GFP

Promoter

PEZ5

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pEZ06

Plasmid

#81130

Purpose

pACSA_J23113_tetR PEZ1_gfp

Depositor

Brian Pfleger

Article

Zess et al Biotechnol Bioeng. 2016 Feb;113(2):424-3

Insert

GFP

Promoter

PEZ1

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pEZ08

Plasmid

#81132

Purpose

pACSA_J23113_tetR PEZ2_gfp

Depositor

Brian Pfleger

Article

Zess et al Biotechnol Bioeng. 2016 Feb;113(2):424-3

Insert

GFP

Promoter

PEZ2

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pEZ05

Plasmid

#81128

Purpose

pACSA_J23117_tetR PEZ1_gfp

Depositor

Brian Pfleger

Article

Zess et al Biotechnol Bioeng. 2016 Feb;113(2):424-3

Insert

GFP

Promoter

PEZ1

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGC295

Plasmid

#19665

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*iA-4xNLS-YFP::let-858(3’)

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pGC292

Plasmid

#19645

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*iA-4xNLS-GFP::let-858(3’)

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC232

Plasmid

#19650

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC296

Plasmid

#19651

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*iA-4xNLS-tdimer2(12)::let-858(3’)

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC309

Plasmid

#19652

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*iA-1xNLS-tdimer2(12)::let-858(3’)

Use

Flp/frt

Tags

1xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC294

Plasmid

#19661

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*iA-4xNLS-CFP::let-858(3’)

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC258

Plasmid

#19664

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC308

Plasmid

#19666

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*iA-1xNLS-YFP::let-858(3’)

Use

Flp/frt

Tags

1xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC382

Plasmid

#19667

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*-YFP::let-858(3’)

Use

Flp/frt

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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CJ34 (miR-1003 3'ssAG->GA)

Plasmid

#17770

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-miR-1003 minigene 3'ssAG->GA

Use

Drosophila p-element vector

Mutation

splice site mutation

Available Since

May 12, 2008

Availability

Academic Institutions and Nonprofits only
pEMdelta

Plasmid

#8487

Depositor

Nancy Craig

Article

Bainton et al Cell 1993 Mar 26;72(6):931-43.

Insert

miniTN7 (tnsA Bacteria)

Expression

Bacterial

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pDblet-swi1+

Plasmid

#15981

Depositor

Eishi Noguchi

Article

Noguchi et al Mol Cell Biol. 2003 Nov . 23(21):7861-74

Insert

Swi1 (swi1 Fission Yeast)

Expression

Yeast

Available Since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only

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pLG173

Plasmid

#124368

Purpose

Control plasmid with only spectinomycin

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

empty backbone (control) (NEWENTRY Synthetic)

Expression

Bacterial

Promoter

No promoter

Availability

Academic Institutions and Nonprofits only

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pMT Draper-GFP

Plasmid

#126653

Purpose

Inducible expression of Draper-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper

Expression

Insect

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Crk-mCherry

Plasmid

#126659

Purpose

Inducible expression of Crk-mCherry in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Crk

Expression

Insect

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT vCrk-GFP

Plasmid

#126661

Purpose

Inducible expression of vCrk truncation in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Crk

Expression

Insect

Mutation

residues 1-182

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Shark-mCherry

Plasmid

#126668

Purpose

Inducible expression of Shark-mCherry in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Shark mCherry

Expression

Insect

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-6xHis-sfGFP

Plasmid

#199171

Purpose

used to express a superfolder GFP in E. coli for comparison to supercharged variants

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

superfolder GFP

Tags

MGHHHHHHGGA

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro

Plasmid

#8452

Purpose

Retroviral vector for shRNA expression.

Depositor

Bob Weinberg

Article

Stewart et al RNA 2003 Apr;9(4):493-501.

Type

Empty backbone

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pVSV eGFP dG

Plasmid

#31842

Depositor

Connie Cepko

Article

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

VSV genome

Expression

Bacterial

Available Since

Oct. 1, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge

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