We narrowed to 15,756 results for: ADA
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Plasmid#163561PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-APOBEC1-UdgX-HF-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…PromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mGas8-PA
Plasmid#176468PurposeExpression vector of mouse growth arrest specific 8 (Gas8) tagged with PA at C-terminus.DepositorInsertgrowth arrest specific 8 (Gas8 Mouse)
UseTagsPAExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shLuc.mKO2
Plasmid#85224PurposeshLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.DepositorInsertFirefly Luciferase
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIneoMyc UNC119Aa
Plasmid#128331PurposeMammalian expression vector of Myc-tagged human UNC119A isoform aDepositorInsertUNC119 (UNC119 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIneoMyc UNC119Ab
Plasmid#128332PurposeMammalian expression vector of Myc-tagged human UNC119A isoform bDepositorInsertUNC119 (UNC119 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-DIO-BiPOLES-mCerulean
Plasmid#154949PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertBiPOLES
UseAAVTagsExpressionMammalianMutationPromoterEf1-alphaAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-eEF2K(S441A/S445A)
Plasmid#110161PurposeExpression of human eEF2K (S441A/S445A) in mammalian cellsDepositorInserteEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)
UseTagsHAExpressionMammalianMutationS441A/S445APromoterCMVAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-eGFP-NLS-RNF168
Plasmid#133977Purposemammalian expression vector of eGFP tagged wild type RNF168DepositorInsertRNF168 (RNF168 Human)
UseTagseGFPExpressionMammalianMutationsiRNA resistant sequence: GAGGAGTCGTGTTTATTGAPromoterCMVAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
PCS4 3XFLAG-PPARgamma2
Plasmid#78770PurposeTo overexpress PPARgamma2 in Mammalian CellsDepositorInsertPPAR_2 (Ppargc1a Mouse)
UseTags3xFLAGExpressionMammalianMutationPromoterCMV IE94Available sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKK-CyOFP1-TEV
Plasmid#105782PurposeExpression of your protein of interest in fusion with orange fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsCyOFP1-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-mClover3-TEV
Plasmid#105778PurposeExpression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).DepositorTypeEmpty backboneUseFlp-in competentTagsmClover3-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSyn-DIO-BiPOLES-mCerulean
Plasmid#154950PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertBiPOLES
UseAAVTagsExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a-Nlux
Plasmid#176239PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux under the control of Ribi promoterDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterRibosomal subunitAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYX233-DHFR
Plasmid#163761PurposeGal-inducible yeast expression of cytosolic DHFRDepositorInsertDHFR (Dhfr Mouse)
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
BcLOV4 fusion tools screening plasmid #1
Plasmid#174511PurposeCloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.DepositorInsert[BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
mDlx-BiPOLES-mCerulean
Plasmid#154946PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertBiPOLES
UseAAVTagsExpressionMammalianMutationPromoterminimal DlxAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_NR1_NR2_LPT1
Plasmid#176253PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene and one spacer targeting the LPAAT geneDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2-TetR-neoR
Plasmid#153310PurposeTet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GWDepositorInsertTetR
UseTransposonTagsExpressionMutationPromoterSV40Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
SECURE miniABEmax-V82G (pJUL1828)
Plasmid#131313PurposeCMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).DepositorInsertbpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGLO-GFP-3UAG
Plasmid#82501PurposeExpresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153DepositorInsertGreen Fluorescence Protein with 3 UAG codons
UseTagsExpressionBacterialMutationAdded an UAG codon at the amino acid position 3, …PromoterAraBADAvailable sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only