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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,835 results for: CAR

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Showing: 4541 - 4560 of 7835 results

  1. HCA1-DuET

    Plasmid
    #213307
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HCA1 (HCAR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213307-plasmid-map-sequence-id-430845

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  3. pJK057

    Plasmid
    #75036
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase, N-terminal His6 (H6EcPurE1) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase (purK Escherichia coli)
    Expression
    Bacterial
    Mutation
    changes residues 2-7 to His (SSRNNP>HHHHHH)
    Promoter
    T7 and n/a
    Available since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    75036-plasmid-map-sequence-id-142299

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  5. pBad-CAts TAG97

    Plasmid
    #105668
    Purpose
    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 97 and C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Publication
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 97
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105668-plasmid-map-sequence-id-205515

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  6. pattB-aubergine-ADH-gf

    Plasmid
    #69448
    Purpose
    genomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid; mutagenesis PCR was used to change the catalytic residue DDH to ADH to express cat-dead Aub
    Depositor
    Phillip Zamore
    Insert
    aubergine (aub Fly)
    Tags
    NA
    Expression
    Insect
    Mutation
    chr2L:10,998,626(−)T>G
    Promoter
    aubergine
    Available since
    Oct. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69448-plasmid-map-sequence-id-122550

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  13. pGEX6P1-human LIC1 G domain

    Plasmid
    #74598
    Purpose
    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389
    Depositor
    Ron Vale
    Publication
    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554
    Insert
    human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
    Tags
    N-terminal GST and Strep II
    Expression
    Bacterial
    Promoter
    T7
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74598-plasmid-map-sequence-id-145957

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  16. pPBbleo-EGF-ScNeo

    Plasmid
    #209902
    Purpose
    To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Publication
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGF-ScNeo (EGF )
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Expression
    Mammalian
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209902-plasmid-map-sequence-id-426731

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  17. pPBbleo-EREG-ScNeo

    Plasmid
    #209905
    Purpose
    To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Publication
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EREG-ScNeo (EREG Human)
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Expression
    Mammalian
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209905-plasmid-map-sequence-id-416688

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Showing: 4541 - 4560 of 7835 results