We narrowed to 20,143 results for: Kin

1. HA-dora[A]_rescue_construct

   Plasmid

   #190640

   Purpose

   Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Use

   Tags

   3xHA

   Expression

   Insect

   Mutation

   Truncated to contain amino acids 1-945

   Promoter

   Actin-5c

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. iSKetSnFR2

   Plasmid

   #140467

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR2, thus enabling optical subcellular pharmacokinetics for S-Ketamine.

   Depositor

   Henry Lester

   Article

   Bera et al Front Cell Neurosci. 2019 Nov 12;13:499.

   Insert

   iSKetSnFR2
   

   Use

   Tags

   His Tag, HA Tag and Myc tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. iSKetSnFR1

   Plasmid

   #140466

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR1, thus enabling optical subcellular pharmacokinetics for S-Ketamine.

   Depositor

   Henry Lester

   Article

   Bera et al Front Cell Neurosci. 2019 Nov 12;13:499.

   Insert

   iSKetSnFR1
   

   Use

   Tags

   His Tag, HA Tag and Myc tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PLK4 G8.1 gRNA

   Plasmid

   #90837

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PLK4

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   PLK4 (Guide Designation G8.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PLK4 G7.1 gRNA

   Plasmid

   #90836

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PLK4

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   PLK4 (Guide Designation G7.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MIS18BP1 F10.1 gRNA

   Plasmid

   #90769

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MIS18BP1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   MIS18BP1 (Guide Designation F10.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MCM6 E1.2 gRNA

   Plasmid

   #90762

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MCM6

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   MCM6 (Guide Designation E1.2)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MASTL F4.1 gRNA

   Plasmid

   #90753

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MASTL

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   MASTL (Guide Designation F4.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. DYNLT3 B3.5 gRNA

   Plasmid

   #90670

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DYNLT3

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   DYNLT3 (Guide Designation B3.5)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DYNLRB1 H11.3 gRNA

    Plasmid

    #90664

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DYNLRB1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    DYNLRB1 (Guide Designation H11.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVR2

    Plasmid

    #84719

    Purpose

    E. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcription

    Depositor

    Nils Walter

    Article

    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

    Inserts

    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    12 nt stretch lacking A's inserted before 5&…

    Promoter

    Available Since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PAK4-3'UTR-BS2

    Plasmid

    #113091

    Purpose

    Luciferase reporter containing PAK4 3' UTR with the second predicted MiR-199a-3p binding

    Depositor

    James Donahue

    Article

    Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

    Insert

    p21 Activated kinase 4 (PAK4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    PGK

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. TGIF2 A11.3 gRNA

    Plasmid

    #90910

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TGIF2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    TGIF2 (Guide Designation A11.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a_6HT-TBP6.7(WT)

    Plasmid

    #112720

    Purpose

    Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(WT)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a_6HT-TBP6.7(P46A)

    Plasmid

    #112721

    Purpose

    This mutation (P46A) marks the first residue within the evolved region of U1A.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(P46A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    P46A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a_6HT-TBP6.7(Q48T)

    Plasmid

    #112725

    Purpose

    This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48T)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48T

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a_6HT-TBP6.7(P51A)

    Plasmid

    #112728

    Purpose

    Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(P51A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    P51A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a_6HT-TBP6.7(Q54A)

    Plasmid

    #112730

    Purpose

    Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q54A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q54A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PAK4-3'UTR-BS1

    Plasmid

    #113090

    Purpose

    Luciferase reporter containing PAK4 3' UTR with the first predicted MiR-199a-3p binding site

    Depositor

    James Donahue

    Article

    Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

    Insert

    p21 Activated kinase 4 (PAK4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    PGK

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TTK H10.1 gRNA

    Plasmid

    #90923

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TTK

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    TTK (Guide Designation H10.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge