We narrowed to 61,832 results for: tra
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Plasmid#164519PurposeAll-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneExpressionMammalianAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only
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pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep
Plasmid#176534PurposeBacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrepDepositorInsertCRISPR-associated IscB (Delaware Bay metagenome)
Tags14xHis-MBP-bdSUMO and TwinStrepExpressionBacterialPromoterT7Available SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLuc-WPRE-HGHpA
Plasmid#87951PurposeRecombinant single-stranded AAV transfer vector expressing Firefly Luciferase under the EF1a promoterDepositorInsertFirefly Luciferase
UseAAVPromoterEF1aAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
GC52 pEGFPN1 anti-Gephyrin nanobody
Plasmid#135224PurposeMammalian expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an intrabodyDepositorInsertanti-Gephyrin (Human) recombinant llama nanobody GC52 (GPHN L. glama (llama))
Tags6xHis-HA-EGFPExpressionMammalianAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA
Plasmid#107313PurposeEncoding aCamkII-mCherry-CreDepositorInsertaCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA
UseAAVExpressionMammalianAvailable SinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF0917
Plasmid#142198PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Flt3-ITD-Y591F/Y919F
Plasmid#74499PurposeRetroviral expression of FLT3 ITD-Y591F/Y919FDepositorInsertFLT3 (FLT3 Human)
UseRetroviralExpressionMammalianMutationY591F/Y919F, T227MPromoterunknownAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKGmDUET-NgBR/hCIT
Plasmid#203173PurposeExpression of human cis-prenyltransferase complex for purificationDepositorTagsStrep-Tag II-TEV and internally 6HIS taggedExpressionMammalianPromoterCMVAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
IC65 pEGFPN1 anti-IRSp53 nanobody
Plasmid#135227PurposeMammalian expression of anti-IRSp53 (Human) recombinant llama nanobody for use as an intrabodyDepositorInsertanti-IRSp53 (Human) recombinant llama nanobody IC65 (BAIAP2 L. glama (llama))
Tags6xHis-HA-EGFPExpressionMammalianAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
DENV4 Rluc Reporter GND mutant Replicon
Plasmid#118586PurposeFor use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.DepositorInsertDENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
UseYeast-e. col shuttle vector prs424MutationThe conserved GDD in the NS5 polymerase gene muta…Available SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFAST-BAC BAF155/SMARCC1-His
Plasmid#177863PurposeTransfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-HisDepositorInsertSMARCC1 (SMARCC1 Human)
TagsHis and His tag at C-terminus with GGGGS linkerExpressionInsectPromoterpolyhedrinAvailable SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF3052
Plasmid#144528PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMega-MaFRSA
Plasmid#200226PurposeThe plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..DepositorInsertsPyrrolysyl-tRNA synthetase
pyrrolysyl-tRNA
TagsnoneExpressionBacterialMutationmutated Asparagine 166 to Alanine and Valine 168 …PromoterproK-lacO and tacIAvailable SinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB-Halo,IRES-NGFR
Plasmid#164520PurposeAll-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneExpressionMammalianAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hARΔ142–337
Plasmid#89106PurposeMammalian expression of human androgen receptor with deletion of amino acids 142-337 N-terminal transactivation function 1DepositorAvailable SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB123 - pL0_rLUC-I (CDS1)
Plasmid#123181PurposeGolden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plantsDepositorInsertluciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
UseLuciferase; Part for plant expressionExpressionPlantMutationNo BpiI and BsaI sites; codon-optimised for plantsAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHDM-SARS-CoV-2 Δ18
Plasmid#164434PurposeEncodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 Spike truncated to remove 18 amino acids (S )
ExpressionMammalianAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-mTagBFP2-rMPC1 shRNA
Plasmid#229016PurposeExpression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfectionDepositorAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-tdTomato
Plasmid#62516PurposeExpresses tdTomato under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing proceduresDepositorInserttdTomato
UseAAV and Synthetic BiologyExpressionMammalianPromoterhUbCAvailable SinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gfp-zf-vasa
Plasmid#167323PurposePlasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.DepositorInsertzf vasa 3'UTRs (ddx4 Zebrafish)
ExpressionBacterialAvailable SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only