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pLenti-X1-Neo-HA-SEC62

Plasmid

#139865

Purpose

Lentiviral expression of HA-tagged human SEC62

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

SEC62 (SEC62 Human)

Use

Lentiviral

Tags

HA

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGal-BE3

Plasmid

#172409

Purpose

Yeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoter

Depositor

Leonid Kruglyak

Article

Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

Insert

BE3

Use

CRISPR

Expression

Yeast

Promoter

GALL

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-Cys-ELP(40nm)-LPETGG

Plasmid

#90475

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

Cys-ELP(40nm)-LPETGG

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-UFM1-dCS

Plasmid

#139869

Purpose

Mammalian expression of HA-tagged human UFM1-cCS

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

UFM1 (UFM1 Human)

Tags

HA

Expression

Mammalian

Mutation

d84-85

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-His6-ODC-Ctag

Plasmid

#163614

Purpose

Expresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.

Depositor

Mark Howarth

Article

Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

Insert

ODC (ODC1 Human)

Tags

C-tag and His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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mbYFPQS

Plasmid

#80742

Purpose

membrane-targeted biosensor for monitoring changes in intracellular chloride

Depositor

Susan Amara, Susan Ingram

Article

Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371

Insert

YFPQS

Tags

palmitoylation signal sequence of neuromodulin

Expression

Mammalian

Mutation

H148Q (CAC to CAG) and V163S (GTG to AGC).

Promoter

CMV

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only
His-AgdNK

Plasmid

#203226

Purpose

For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

Depositor

Vern Schramm

Article

Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

Insert

deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)

Tags

N-terminal TEV cleavable 6xHis tag

Expression

Bacterial

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2 (S939A)

Plasmid

#14132

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 S939A (TSC2 Human)

Tags

Flag

Expression

Mammalian

Mutation

S939A.

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pMT448

Plasmid

#139388

Purpose

Bile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1), pNBU1 backbone, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

Bile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1),

Use

Synthetic Biology

Promoter

Bile Acid inducible Promoter

Available Since

Jan. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMT450

Plasmid

#139390

Purpose

Bile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

Bile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR

Use

Synthetic Biology

Promoter

Bile Acid inducible Promoter

Available Since

Jan. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMT492

Plasmid

#139397

Purpose

Bile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5), pNBU1 backbone, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

Bile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5)

Use

Synthetic Biology

Promoter

Bile Acid inducible Promoter

Available Since

Jan. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-GGG-ELP(40nm)-Cys

Plasmid

#91571

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-ELP(40nm)-Cys

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2 (T1462A)

Plasmid

#14130

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 T1462A (TSC2 Human)

Tags

Flag

Expression

Mammalian

Mutation

T1462A

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pMT493

Plasmid

#139398

Purpose

Bile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6), pNBU1 backbone, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

Bile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6)

Use

Synthetic Biology

Promoter

Bile Acid inducible Promoter

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMT494

Plasmid

#139399

Purpose

Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7), pNBU1 backbone, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7)

Use

Synthetic Biology

Promoter

Bile Acid inducible Promoter

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMT455

Plasmid

#139392

Purpose

aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate), AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate)

Use

Synthetic Biology

Promoter

aTC and BA inducible promoters

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-TEX264-HA

Plasmid

#139866

Purpose

Lentiviral expression of HA tagged (C terminal) human TEX264

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

TEX264 (TEX264 Human)

Use

Lentiviral

Tags

HA

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-SAG1-DHFR

Plasmid

#80322

Purpose

Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

SAG1 sgRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-GtACR2

Plasmid

#85463

Purpose

Natural anion conducting Channelrhodopsin with blue absorption. Closing kinetics 60ms. High conductivity. Codon optimized for mammalian expression (human/mouse)

Depositor

Peter Hegemann

Article

Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

Insert

Synthetic construct ACR2 gene

Tags

mCherry

Expression

Mammalian

Promoter

CMV (+enhancer)

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-GtACR1

Plasmid

#85464

Purpose

Natural anion conducting Channelrhodopsin with cyan absorption. Closing kinetics 140ms. High conductivity. Codon optimized for mammalian expression (human/mouse)

Depositor

Peter Hegemann

Article

Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

Insert

Synthetic construct ACR1 gene

Tags

mCherry

Expression

Mammalian

Promoter

CMV (+enhancer)

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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