We narrowed to 11,632 results for: Vars

1. pCDB181

   Plasmid

   #80692

   Purpose

   for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHEE_06

   Depositor

   David Baker

   Article

   Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

   Insert

   gEHEE_06
   

   Tags

   OsmY-10His-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSECB sgMTHFD2L_1

   Plasmid

   #106306

   Purpose

   Express Cas9 and sgRNA targeting MTHFD2L

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting MTHFD2L (MTHFD2L Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSECB sgMTHFD2L_2

   Plasmid

   #106307

   Purpose

   Express Cas9 and sgRNA targeting MTHFD2L

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting MTHFD2L (MTHFD2L Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. LacI/109-pHG165c

   Plasmid

   #90058

   Purpose

   WT LacI polymorphism with T at position 109

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LacI/109
   

   Expression

   Bacterial

   Mutation

   109

   Promoter

   Iq

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p35S::IRE1a

   Plasmid

   #135232

   Purpose

   constitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)

   Depositor

   Armin Djamei

   Article

   Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

   Insert

   IRE1a (IRE1A Mustard Weed)
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pY71-PT7-RiboJ-sfGFP-MGapt

   Plasmid

   #129119

   Purpose

   Expresses a fluorescent reporter for the quantification of cell-free protein expression.

   Depositor

   Matthew Lux

   Article

   Cole et al ACS Synth Biol. 2019 Sep 20;8(9):2080-20

   Insert

   Superfolder GFP with Malachite Green aptamer
   

   Use

   Synthetic Biology

   Tags

   His6 and RiboJ Insulator

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pnEA-vH_His-TEV-DSep1

   Plasmid

   #174493

   Purpose

   bacterial expression of Drosophila Sep1

   Depositor

   Manos Mavrakis

   Article

   Mavrakis et al Nat Cell Biol. 2014 Apr;16(4):322-34. do

   Insert

   Sep1 (Sep1 Fly)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPKm-293

   Plasmid

   #105817

   Purpose

   pcDNA - TET DBD - MTAD. Expresses TET DNA binding domain fused to MTAD

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   TET DBD and MTAD
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEMS2131

   Plasmid

   #111895

   Purpose

   ssAAV genome with MCS for inserting MiniP promoter to drive an emerald GFP (EmGFP) reporter. Contains WPRE

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Type

   Empty backbone

   Use

   AAV

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPKm- 226

    Plasmid

    #90497

    Purpose

    pcDNA3 - CMVmin - PIF3 - VPR expresses PIF3 and VPR transactivator domain , under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    PIF3-VP64
    

    Expression

    Mammalian

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL

    Plasmid

    #63886

    Purpose

    An AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.

    Depositor

    Brandon Harvey

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    ER-localized GCaMP3(wt)
    

    Use

    AAV

    Promoter

    human SYN1

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_PSTPIP1-1/1

    Plasmid

    #91453

    Purpose

    Protein expression and purification of human SH3 domain construct PSTPIP1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PSTPIP1-1/1 (PSTPIP1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mKO-Fibrillarin-7

    Plasmid

    #57842

    Purpose

    Localization: Nuclear - Nucleolus, Excitation: 548, Emission: 561

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Fibrillarin (FBL Human)
    

    Tags

    mKO

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEMS1964

    Plasmid

    #82574

    Purpose

    High copy number plasmid containing Ple305 MiniPromoter insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple305
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Available Since

    Sept. 23, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. CoV2-N-P13L

    Plasmid

    #177946

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P13L). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P13L) (N )
    

    Expression

    Mammalian

    Mutation

    P13L

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. CoV2-N-P80R

    Plasmid

    #177947

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P80R) (N )
    

    Expression

    Mammalian

    Mutation

    P80R

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CoV2-N-S235F

    Plasmid

    #177956

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (S235F) (N )
    

    Expression

    Mammalian

    Mutation

    S235F

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103_PIK3R1-1/1

    Plasmid

    #91274

    Purpose

    Protein expression and purification of human SH3 domain construct PIK3R1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PIK3R1-1/1 (PIK3R1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCFJ2453 - [1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)

    Plasmid

    #159863

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_NEDD9-1/1

    Plasmid

    #91498

    Purpose

    Protein expression and purification of human SH3 domain construct NEDD9-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NEDD9-1/1 (NEDD9 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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