We narrowed to 36,681 results for: kan

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pDD131

Plasmid

#119886

Purpose

Optimized aTc inducible pTet promoter for Rhodococcus opacus expressing mCherry on pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

Insert

pTet_mCherry

Expression

Bacterial

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMK365 (Tet-OsTIR1-loxP-PURO-loxP)

Plasmid

#121185

Purpose

OsTIR1 introduction

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Tet-OsTIR1-loxP-PURO-loxP

Use

CRISPR

Mutation

OsTIR1 codon optimized for human cells

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_hU6

Plasmid

#102840

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_hU6promoter

Promoter

hU6

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pDD57

Plasmid

#119882

Purpose

Constitutive expression of GFP+ on pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pConstitutive_GFP+

Expression

Bacterial

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-His-TEV-NQO1

Plasmid

#119058

Purpose

For expression in E.coli and affinity purification of N-terminally His tagged NQO1

Depositor

Yosef Shaul

Article

Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Tags

6His, TEV site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pTP261

Plasmid

#104166

Purpose

Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)

Tags

14x Histidine tag and NEDD8 from Brachypodium dis…

Expression

Bacterial

Mutation

1x Cysteine located at bps 493-495 in full sequen…

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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ITPKA_3xmScarletI

Plasmid

#112960

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

ITPKA

Tags

3x mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1-mCherry

Plasmid

#102629

Purpose

In vitro expression of mCherry from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mCherry

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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VEGFR2-YFP

Plasmid

#108853

Purpose

Encodes for human VEGFR2 fluorescently labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linker

Depositor

Kalina Hristova

Article

Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

Insert

KDR (KDR Human)

Tags

Labeled with eYFP on the C-Terminus via a 3 amino…

Expression

Mammalian

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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cyto-YFP-FKBPx5

Plasmid

#103777

Purpose

expression of iPOLYMER component in mammalian cells; has nuclear exclusion sequence

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

cyto-YFP-FKBPx5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

Plasmid

#99278

Purpose

Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance

Depositor

Jordan Miller

Article

Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

Inserts

H2B-mVenus-IRES-mCherry
Puro

Use

Lentiviral

Tags

H2B, IRES, PGK, mCherry, and mVenus

Promoter

EF1 alpha-human and PGK-mouse

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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HIP1R-GFP-FKBP

Plasmid

#100752

Purpose

Expression of HIP1R with a GFP-FKBP tag in mammalian cells.

Depositor

Stephen Royle

Article

Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

Insert

HIP1R (HIP1R Human)

Tags

FKBP and GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pIRES-hrGFP II-TET1_CD

Plasmid

#83570

Purpose

Expresses the catalytic domain of TET1 in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

TET1 amino acids 1-1417 deleted

Promoter

CMV

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pDIRECT_25F

Plasmid

#91143

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA

Use

CRISPR

Expression

Plant

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3

Plasmid

#89975

Purpose

Expresses GFP-tagged ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab8a-QL

Plasmid

#86076

Purpose

A mammalian expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed glutamine 67 to leucine

Promoter

CMV

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-PH(Akt2)

Plasmid

#86596

Purpose

Expresses mCherry-tagged PH(WT) domain of human Akt2

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2 PH domain (AKT2 Human)

Expression

Mammalian

Mutation

Amino acids 1 - 108

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21-T31N (DN)

Plasmid

#83423

Purpose

Mouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Residue 31 from T to N (T31N: ACG -> AAC)

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pME-mTagBFP

Plasmid

#75148

Purpose

TagBFP (blue) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mTagBFP

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEB1deltaC-mCherry

Plasmid

#80603

Purpose

Expresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus end

Depositor

Torsten Wittmann

Article

Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

Insert

EB1 (MAPRE1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Truncated C-terminus; EB1 coding sequence ends at…

Promoter

CMV

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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