We narrowed to 6,134 results for: nls

1. 3xnls-G-Ca-FLITS

   Plasmid

   #191463

   Purpose

   Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   G-Ca-FLITS
   

   Use

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-P4:gs:nls:VPR

   Plasmid

   #135983

   Purpose

   Expresses the P4 coiled coil fused to the VPR transcriptional activation domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   P4 coiled-coil fused to the VPR activation domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti_Efs_hSpCas9_NLS_FLAG-WPRE

   Plasmid

   #97313

   Purpose

   Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Lentiviral and Mouse Targeting

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJIT163-2NLSCas9

   Plasmid

   #53064

   Purpose

   Expression of rice codon-optimized Cas9 in plant cells

   Depositor

   Caixia Gao

   Article

   Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

   Insert

   Cas9
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   Rice codon optimized

   Promoter

   2x35S

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR

   Plasmid

   #81251

   Purpose

   Lentiviral construct for building stable cell lines expressing Cas9 via puromycin selection

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   Cas9-3xNLS
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-NLS-ZapCV2

   Plasmid

   #203664

   Purpose

   Expresses zinc sensor ZapCV2 in the nucleus of mammalian cells

   Depositor

   Amy Palmer

   Article

   Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

   Insert

   ZapCV2 Zinc sensor
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2xNLS-pMJ915v2

   Plasmid

   #88916

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. BB1_23_dCAS9(3xFLAG_SV40NLS)

   Plasmid

   #161478

   Purpose

   BB1 encoding a FLAG tagged dCAS9 with a SV40 NLS

   Depositor

   Diethard Mattanovich, Brigitte Gasser

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Insert

   dCAS9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   FLAG Tag, SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3E-nlsGFP no-pA

   Plasmid

   #80815

   Purpose

   nlsGFP without pA

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   p3E-CMV-nlsGFP no pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAi14-GFPNLS-MC

    Plasmid

    #87114

    Purpose

    minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    GFPNLSPpA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    nEF

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMVMagFRET-1-NLS

    Plasmid

    #50748

    Purpose

    Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

    Insert

    gene encoding for MagFRET-1 with NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR'CMVGFP_NLS_hRIF1(1-1308)

    Plasmid

    #23134

    Depositor

    Lifeng Xu

    Article

    Xu et al J Cell Biol. 2004 Dec 6. 167(5):819-30.

    Insert

    RIF1 deletion construct (RIF1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-21a_2xNLS_LbCpf1_protein_expression

    Plasmid

    #114366

    Purpose

    Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

    Insert

    2xNLS_LbCpf1
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

    Plasmid

    #79372

    Purpose

    Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    GCN4-sfGFP-p65-hsf1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR_TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP

    Plasmid

    #162233

    Purpose

    Lentiviral vector for the expression of GFP nanobody LaG16LaG2 -IFP : TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    anti-GFP nanobody lag16lag2 and IFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p16 nlsGPS1-NR

    Plasmid

    #101082

    Purpose

    GA biosensor expression plasmid

    Depositor

    Wolf Frommer, Alexander Jones

    Article

    Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

    Insert

    nlsGPS1-NR
    

    Use

    Tags

    cMyc

    Expression

    Plant

    Mutation

    AtGAI E51K E54R, AtGID1C S114A F115A

    Promoter

    p16 (AT3G60245)

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF1a_MYC deltaNLS_P2A_Hygro_Barcode

    Plasmid

    #120506

    Purpose

    Barcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC H84NLS (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of nuclear localization signal sequence

    Promoter

    EF1a

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWPT-nlsLacZ

    Plasmid

    #12261

    Depositor

    Didier Trono

    Insert

    LacZ
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. 1xNLS-pMJ915v2

    Plasmid

    #88915

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMVMagFRET-6-NLS

    Plasmid

    #50757

    Purpose

    Expresses MagFRET-6 in the nucleus of mammalian cells. Weak affinity control FRET sensor for MagFRET-1

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

    Insert

    gene encoding for MagFRET-6 with NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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