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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 441 - 460 of 6562 results

  2. pNLS-2xBFP(4C)

    Plasmid
    #176151
    Purpose
    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.
    Depositor
    Siegfried Musser
    Publication
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    NLS-2xBFP(4C)
    Tags
    6xHis tag and SV40 nuclear localization sequence …
    Expression
    Bacterial
    Mutation
    Compared with EGFP, the two BFP domains have the …
    Promoter
    Ptrc
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    176151-plasmid-map-sequence-id-356068

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  3. pRD439

    Plasmid
    #168465
    Purpose
    For the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system
    Depositor
    Remus Dame
    Publication
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-mTurquoise2-H-NSdbd-NLS
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168465-plasmid-map-sequence-id-331915

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  4. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    107943-plasmid-map-sequence-id-207614

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  14. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    139090-plasmid-map-sequence-id-273886

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  15. pCMV-PE2

    Plasmid
    #132775
    Purpose
    Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b
    Depositor
    David Liu
    Publication
    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    See manuscript
    Promoter
    CMV
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127 Articles
    132775-plasmid-map-sequence-id-259720

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  16. pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3

    Plasmid
    #73205
    Purpose
    Expresses myc-dL5(E52D)-mCer3 fusion protein in nuclei of mammalian cells. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Publication
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-myc-dL5-2XG4S-mCer3 (MYC Synthetic, Human)
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73205-plasmid-map-sequence-id-139718

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  17. pWPT-nlsLacZ

    Plasmid
    #12261
    Depositor
    Didier Trono
    Insert
    LacZ
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    12261-plasmid-map-sequence-id-7273

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Showing: 441 - 460 of 6562 results