We narrowed to 2,314 results for: tub
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Plasmid#180279PurposePlasmid encoding the human protease OTUB1 without a tag. Polycistronic red fluorescent retroviral vector (unmodified from TAKARA BIO).DepositorInserthuman Otubain-1 (OTUB1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Clim Tub MCH (CTM)
Plasmid#104445PurposeExpresses mCherry under tubulin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.DepositorInsertmCherry
UseExpression in corallochytrium (single-celled eukaā¦TagsExpressionMutationPromotertubulin promoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK-S. tuberosum trnV/3'rps12
Plasmid#123585PurposeMoChlo kitDepositorInsertS. tuberosum trnV/3'rps12 integration site
UseChloroplast transformation vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-S. tuberosum trnG/trnfM
Plasmid#123584PurposeMoChlo kitDepositorInsertS. tuberosum trnG/trnfM integration site
UseChloroplast transformation vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
gRNAs[bTub+Tra].1046D
Plasmid#112693Purposeexpress two gRNA targeting bTub & Tra under dU6-3 promoterDepositorInsertU6.3-gRNA[bTub] & U6.3-gRNA[Tra] (tra Fly)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
gRNA[bTub+DsxF].1046A
Plasmid#112694Purposeexpress two gRNA targeting bTub & DsxF under dU6-3 promoterDepositorInsertU6.3-gRNA[bTub] & U6.3-gRNA[DsxF] (dsx Fly)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
gamma-tubulin (334-451)
Plasmid#104436Purposeexpressing a fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLSDepositorInsertgamma-tubulin fragment 334 to 451 (TUBG1 Human)
UseTagsNoneExpressionMammalianMutationdeleted a.a. 1 to 333PromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9 (BB)-2A-GFP gamma-tubulin sg
Plasmid#104437PurposeKnockdown the expression of gamma-tubulin in human cellsDepositorInsertgamma-tubulin sgRNA
UseTagsThe gamma-tubulin sgRNA is coexpressed with 3xFLAā¦ExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
Xla.Tubb2:hArr-TEV;he1.1:eYFP
Plasmid#218859PurposeXla.Tubb2 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.DepositorInserthArr
UseTagshuman Arrestin-Tobbaco Etch Virus protease fusionā¦ExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP
Plasmid#218857PurposeXla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.DepositorInserthGCGR (GCGR Human)
UseTagsQF fusion proteinExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β2B Tubulin
Plasmid#170553PurposeEncodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.DepositorUseTagsAP linker, GS linker (GGSGG), His10 tag, L21 enhaā¦ExpressionInsectMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTUB1:OsTIR1-3FLAG, SAG1:CAT
Plasmid#87258PurposeTIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.DepositorInsertsTIR1
CAT
UseToxoplasma expressionTags3FLAGExpressionMutationCodon optimized for Toxoplasma gondii expressionPromoterTgSAG1 361 bp and TgTUB1 2717 bpAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUB1 (full-length, aa 1-271)
Plasmid#61420PurposeExpresses human OTUB1 (full-length) in E. coli.DepositorInsertOTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationPromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2
Plasmid#191338PurposeExpresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice culturesDepositorInsertTUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-AtUBQ10:HR1b-GFP-T35S
Plasmid#211837PurposePlant transient expression cassette for HR1b-GFP fusion under UBQ10 promoter.DepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationPromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)
Plasmid#160629PurposeTU for the expression of LbCas12aDepositorInsertAtUBQ10-:LbCpf1(Cas12a):Tnos
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterUBQ10Available sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-N-gamma-tubulin (334-449)
Plasmid#87859PurposeFluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLSDepositorInsertgamma-tubulin 1 (TUBG1 Human)
UseTagsGFPExpressionMammalianMutationfragment from aa 334 to 449PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE GS-gRNA (hSyn-Cre) mTubb3
Plasmid#200369PurposePositive control plasmid expressing mouse Tubb3 GS-gRNA (with hSyn-Cre)DepositorInsertMouse Tubb3 gRNA and Cre
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 1 CMV EYFP Tubulin
Plasmid#206254PurposeENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes EYFP Tubulin under the control of CMV promoter.DepositorInsertEYFP Tubulin
UseMultimate/gateway entr 1TagsEYFPExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)
Plasmid#171089PurposeCRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.DepositorInsertsDecoy sgRNA
dCas9
Chloramphenicol acetyltransferase
UseCRISPR; Toxoplasma gondii expressionTags3X FLAG and Nuclear Localization SignalExpressionMutationD10A, H840APromoterTUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondiā¦Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only