163,331 results

1. pMIR-FLAG-MLL-AF9

   Plasmid

   #71444

   Purpose

   Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

   Depositor

   Daisuke Nakada

   Article

   Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

   Insert

   MLL-AF9 (KMT2A Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-fDIO EYFP (AAV1)

   Viral Prep

   #55641-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Promoter

   EF1a

   Tags

   EYFP (Flp-dependent)

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

3. pCFT4.2

   Plasmid

   #208807

   Purpose

   Expresses the fluorescent protein phiLOV2.1

   Depositor

   François-Xavier Campbell-Valois

   Article

   Tchagang et al Front Microbiol. 2023 Oct 20;14:1245755.

   Insert

   phiLOV2.1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6/V5-DEST-MTHFD2

   Plasmid

   #31213

   Depositor

   Lynda Chin

   Article

   Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

   Insert

   MTHFD2 (MTHFD2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPR-mCherry

   Plasmid

   #75161

   Purpose

   Derived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNLS-iRFP670

   Plasmid

   #45466

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP670
   

   Use

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

   Promoter

   CMV

   Available since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAdEasy-1

   Plasmid

   #16400

   Purpose

   Adenoviral vector for use in AdEasy System.

   Depositor

   Bert Vogelstein

   Article

   He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDRM210 LGP (Luciferase GFP Puro)

   Plasmid

   #174722

   Purpose

   Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   Firefly Luciferase
   eGFP
   pac
   

   Use

   Lentiviral and Luciferase

   Tags

   E2A linker and F2A linker

   Expression

   Mammalian

   Mutation

   Promoter

   MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMJ920

   Plasmid

   #42234

   Depositor

   Jennifer Doudna

   Article

   Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   GFP, HA epitope, and NLS (nuclear localization si…

   Expression

   Mammalian

   Mutation

   codon-optimized synthetic DNA sequence

   Promoter

   CMV

   Available since

   March 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 3xFlag IFIT1

    Plasmid

    #53554

    Purpose

    mammalian expression of IFIT1

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT1 (IFIT1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

    Plasmid

    #84561

    Purpose

    pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops

    Depositor

    Robert Singer

    Article

    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

    Insert

    FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
    

    Use

    Lentiviral

    Tags

    Flag, Suntag, oxBFP, AID (auxin-inducible degron)

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. PHB2-mCherry

    Plasmid

    #232785

    Purpose

    Encodes an mCherry-tagged Prohibitin2/PHB2

    Depositor

    Giulia Bertolin

    Article

    Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

    Insert

    PHB2 (PHB2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)

    Plasmid

    #126580

    Purpose

    A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    OsARF16-PB1 domain
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmGFP-ADAR2

    Plasmid

    #117929

    Purpose

    ADAR2 expression plasmid.

    Depositor

    Kumiko Ui-Tei

    Article

    Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

    Insert

    ADAR2 (ADARB1 Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA

    Plasmid

    #113691

    Purpose

    U6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK1334

    Plasmid

    #127965

    Purpose

    sgRNA expression vector compatible with CROP-Seq and imaging screens

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. V2R-GFP

    Plasmid

    #208910

    Purpose

    encodes the human vasopressin receptor 2, tagged with GFP

    Depositor

    Christian Gruber

    Article

    Di Giglio et al Sci Rep. 2017 Feb 1;7:41002. doi: 10.103

    Insert

    AVPR2 (AVPR2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag

    Plasmid

    #209652

    Purpose

    Expression of iATPSnFR2, medium affinity

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95K.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A95K

    Promoter

    CAG

    Available since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (AAV1)

    Viral Prep

    #50942-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (#50942). In addition to the viral particles, you will also receive purified pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate mRuby2 expression under a human synapsin1 promoter. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Tobias Bonhoeffer, Tobias Rose, Mark Huebener

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Promoter

    Syn

    Tags

    mRuby2

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

20. Integrin β4-GFP

    Plasmid

    #205092

    Purpose

    Express GFP-tagged human integrin β4 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 4 (ITGB4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-SynB

    Plasmid

    #174861

    Purpose

    Expresses mouse syncytin B in mammalian cells

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    syncytin B (Synb Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDEST47-ARL2-GFP

    Plasmid

    #67396

    Purpose

    Mammalian expression of hArl2 with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL2 (ARL2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pEVOL-pylT-N346A/C348A

    Plasmid

    #127411

    Purpose

    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers

    Depositor

    Wenshe Liu

    Article

    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3

    Insert

    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N346A-C348A

    Promoter

    araBAD

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pSDM052

    Plasmid

    #194341

    Purpose

    pUC mini-Tn7 vector expressing LseCas13a

    Depositor

    Joseph Bondy-Denomy

    Article

    Mendoza et al Nature. 2020 Jan;577(7789):244-248. doi:

    Insert

    Cas13a(Lse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-hsyn-GRAB_Ado1.0

    Plasmid

    #153285

    Purpose

    Express the adenosine sensor GRAB_Ado1.0 in neurons

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine sensor GRAB_Ado1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. Mouse Metabolism Library

    Pooled Library

    #163966

    Purpose

    Lentiviral CRISPR library targets 2,918 mouse metabolic genes with ~18,000 guide RNAs. The library contains ~6 gRNAs per gene.

    Depositor

    Alec Kimmelman

    Article

    Biancur et al Cell Metab. 2020 Oct 28. pii: S1550-4131

    Use

    CRISPR and Lentiviral

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

27. pCAG-VSVL

    Plasmid

    #64085

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain polymerase gene

    Depositor

    Ian Wickersham

    Insert

    VSV polymerase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMRX-No-HaloTag7-mGFP-LC3-mRFP

    Plasmid

    #184902

    Purpose

    Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Use

    Tags

    HaloTag7, monomeric EGFP, and monomeric RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV 3xFLAG-LC3C WT

    Plasmid

    #123095

    Purpose

    Expresses 3xFLAG-LC3C wild-type in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGP-CMV-GCaMP6s-CAAX

    Plasmid

    #52228

    Purpose

    Plasma membrane targeted GCaMP6s, calcium sensor

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    GCaMP6s-CAAX
    

    Use

    Tags

    6xHis and CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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