We narrowed to 9,737 results for: Ada;

1. pAV10.K4

   Plasmid

   #63212

   Purpose

   pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

   Plasmid

   #79619

   Purpose

   Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   3xFlag-PmCDA1

   Expression

   Mammalian

   Mutation

   D10A for nickase Cas9

   Promoter

   pCMV

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

   Plasmid

   #132560

   Purpose

   Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

   Depositor

   Baisong Lu

   Article

   Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

   Inserts

   ABEmax
   sgRNA, with Tetraloop replaced by com aptamer
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2-ER-EmEGFP

   Plasmid

   #169000

   Purpose

   Express ER-EmEGFP in zebrafish

   Depositor

   Noboru Mizushima

   Article

   Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

   Insert

   ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Synthetic, Human)
   

   Use

   Unspecified

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAV11K

   Plasmid

   #63184

   Purpose

   pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4-RI1-332-MYC-His6

   Plasmid

   #185351

   Purpose

   Mammalian expression of Ribophorin I

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   Ribophorin I (RPN1 Human)
   

   Use

   Tags

   Myc, 6xHis

   Expression

   Mammalian

   Mutation

   aa 1-332 only

   Promoter

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBItetDT12nGFP

   Plasmid

   #80420

   Purpose

   tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 12 CTG repeats in exon 15 located

   Depositor

   Thomas Cooper

   Article

   Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   EGFP and human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-PITCh-H3C2

   Plasmid

   #207780

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of H3C2 (H3C2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MAC_N_ELK3

   Plasmid

   #167750

   Purpose

   Expresses ELK3 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   ELK3 (ELK3 Human)
   

   Use

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-rtTA-zeo

    Plasmid

    #183233

    Purpose

    Lentiviral vector expressing the rtTa gene

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    rtTA-T2A-Zeo
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79621

    Purpose

    Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Mammalian

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pCMV

    Available since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLENTI_RPS3_K214R

    Plasmid

    #127132

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS3 (RPS3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K214R

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5FRT/TO-DVC1-Strep-HA

    Plasmid

    #113481

    Purpose

    Expression of human DVC1 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    DVC1 (SPRTN Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. CytERM-mAvicFP1

    Plasmid

    #129510

    Purpose

    Expresses CytERM-mAvicFP1 in mammalian cells (for OSER assay)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    CytERM-mAvicFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDisplay-GRAB_OTmut

    Plasmid

    #185385

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cells

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEX- HcRed -hLC3ΔG

    Plasmid

    #24992

    Depositor

    Isei Tanida

    Article

    Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

    Insert

    a mutant human MAP1-LC3B fused with HcRed (MAP1LC3B Human)
    

    Use

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    deleted Gly at 121

    Promoter

    Available since

    June 28, 2010

    Availability

    Academic Institutions and Nonprofits only

17. Myc-Cdh1

    Plasmid

    #28127

    Depositor

    Pumin Zhang

    Article

    Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

    Insert

    fzr1 (FZR1 Human)
    

    Use

    Mouse Targeting and Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-HA-RIPK1 K115R

    Plasmid

    #78835

    Purpose

    To overexpress RIPK1 K115R in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K115R

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE2-TetOminiCMV-Oct4

    Plasmid

    #136611

    Purpose

    Dox-inducible lentiviral vector expressing mouse Oct4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCold I-Hero45

    Plasmid

    #187931

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero45

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero45 (SERBP1 Human)
    

    Use

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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