We narrowed to 10,236 results for: ada

1. pPB9v1

   Plasmid

   #210028

   Purpose

   Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas9
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MAC_N_ETV4

   Plasmid

   #167753

   Purpose

   Expresses ETV4 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   ETV4 (ETV4 Human)
   

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Biomolecular FeedBack (FB)

   Plasmid

   #109391

   Purpose

   This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

   Insert

   pJ23100mut-dCas9-T- phtpG1-sgRNA
   

   Expression

   Bacterial

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NO- DSD Sensor 2

   Plasmid

   #199786

   Purpose

   Synthetic gene circuit

   Depositor

   Timothy Lu

   Article

   Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

   Insert

   NO- DSD Sensor
   

   Use

   Synthetic Biology

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-hSyn-OsTIR1(F74A)

   Plasmid

   #140731

   Purpose

   OsTIR1(F74A)-P2A-mAID-EGFP-NES

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   OsTIR1(F74A)-P2A-mAID-EGFP-NES
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX335-EN475

   Plasmid

   #86231

   Purpose

   For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   spCas9-nickase and sgRNA against mouse CTCF STOP Codon
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)

   Plasmid

   #191164

   Purpose

   Similar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequences

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:s70587:EGFP (additional cutsites)
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX335-EN477

   Plasmid

   #86232

   Purpose

   For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   spCas9-nickase and sgRNA against mouse CTCF STOP Codon
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. px458_2A_GFP_sgRNA_PELO

   Plasmid

   #127124

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   gRNA PELO (PELO Human)
   

   Use

   CRISPR

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMK427 (OsTIR1(WT) mAID-EGFP-Nluc)

    Plasmid

    #140658

    Purpose

    OsTIR1(WT)-P2A-mAID-EGFP-Nluc

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(WT) mAID-EGFP-Nluc TOL2
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-myc-ARF

    Plasmid

    #19930

    Depositor

    Yue Xiong

    Article

    Zhang et al Cell. 1998 Mar 20. 92(6):725-34.

    Insert

    cyclin-dependent kinase inhibitor 2A p19ARF (CDKN2A Human)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pdCas12a(RuvC)

    Plasmid

    #186447

    Purpose

    Encodes catalytically inactive Cas12a (D917A, E1006A) from F. novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    dCas12a
    

    Use

    CRISPR

    Mutation

    D917A, E1006A

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQ265E9

    Plasmid

    #213472

    Purpose

    Gateway entry clone (attL1 & attR5) TadDE-32aa linker-zSpCas9-D10A-2xUGI for C-T and A to G base editing

    Depositor

    Yiping Qi

    Article

    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

    Insert

    TadDE-32aa linker-zSpCas9-D10A-2xUGI
    

    Use

    CRISPR; Gateway compatible tadde-32aa linker-zspc…

    Tags

    NO

    Expression

    Plant

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3 barr1 HA

    Plasmid

    #14693

    Depositor

    Robert Lefkowitz

    Article

    Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

    Insert

    beta arrestin 1 (Arrb1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

15. W118-1_hTGM4-Flag

    Plasmid

    #180409

    Purpose

    lentiviral transduction of human TGM4 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM4 (TGM4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. W118-1_hTGM5-V5

    Plasmid

    #180414

    Purpose

    lentiviral transduction of human TGM5 (with a C-terminal V5 tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM5 (TGM5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psigma1-EGFP

    Plasmid

    #53611

    Purpose

    Expresses sigma1 of adaptor protein of AP1

    Depositor

    Tom Kirchhausen

    Article

    Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

    Insert

    sigma 1 (Ap1s1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBABEPuro-HA-GLI1

    Plasmid

    #62967

    Purpose

    Expresses HA-tagged Gli1

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    GLI1 (GLI1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pGEX-gp78c/2767

    Plasmid

    #11429

    Depositor

    Allan Weissman

    Article

    Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

    Insert

    gp78c (AMFR Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    n/a

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pGEMHE-mCherry-mTrim21ΔPRYSPRY

    Plasmid

    #105524

    Purpose

    T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mCherry

    Mutation

    deletion of PRYSPRY domain (aa1-281)

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge