We narrowed to 32,724 results for: eng

1. PAJ408

   Plasmid

   #104150

   Purpose

   dCas9 plasmid expressing full length mKate2 mRNA without RBS

   Depositor

   Alfonso Jaramillo

   Article

   Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   pLtetO-1

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. GGDestX2-Amp

   Plasmid

   #157654

   Purpose

   Golden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNA

   Depositor

   Karmella Haynes

   Article

   Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   U6, CBh

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUP95Q15AL460PGW (E3)

   Plasmid

   #74033

   Purpose

   lentiviral expression of Psd95-Q15A L460P-EGFP fusion

   Depositor

   Robert Malenka, Oliver Schluter, Weifeng Xu

   Article

   Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

   Insert

   Psd95
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Q15A, L460P

   Promoter

   hUbC

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

4. pXf20pemIK-GW

   Plasmid

   #126576

   Purpose

   Vector for gene expression in Xylella fastidiosa, includes pemI/pemK toxin-antitoxin system for plasmid stability, Gateway cloning compatible

   Depositor

   Lindsey Burbank, Drake Stenger

   Article

   Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUP95DPESTGW (C7)

   Plasmid

   #74023

   Purpose

   lentiviral expression of Psd95-DPEST-EGFP fusion

   Depositor

   Robert Malenka, Oliver Schluter, Weifeng Xu

   Article

   Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

   Insert

   Psd95
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   DPEST; amino acids 10-25 deleted

   Promoter

   hUbC

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

6. pGGB005

   Plasmid

   #48822

   Purpose

   Provides the SV40 nuclear localization signal as GreenGate N-terminal tag module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   SV40 nuclear localization signal
   

   Use

   Golden gate compatible cloning vector

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGGA003

   Plasmid

   #48814

   Purpose

   Provides the WUS promoter as GreenGate module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   WUS promoter
   

   Use

   Golden gate compatible cloning vector

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFPxm163

   Plasmid

   #193480

   Purpose

   Turn-off fluorescent sensor for chloride

   Depositor

   Sheel Dodani

   Article

   Peng et al Chem Sci. 2022 Oct 6;13(43):12659-12672.

   Insert

   GFPxm163
   

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. hCas9

   Plasmid

   #41815

   Purpose

   Expresses human codon optimized Cas9 nuclease for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ef1a_Large T-antigen_Ires_Puro

    Plasmid

    #18922

    Depositor

    Linzhao Cheng

    Article

    Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

    Insert

    Large T Antigen (SV40gp6 Simian virus 40)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ai9

    Plasmid

    #22799

    Purpose

    Rosa26 targeting vector, Cre-dependent tdtomato expression. See article for more colors.

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

    Insert

    CAG-Floxed tdTomato
    

    Use

    Mouse Targeting

    Available Since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hsyn-GRAB_Ado1.0mut

    Plasmid

    #153287

    Purpose

    Express the adenosine-insensitive control sensor GRAB_Ado1.0mut in neurons

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNA_AAVS1-T2

    Plasmid

    #41818

    Purpose

    Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_AAVS1-T2
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiSAMPHv2

    Plasmid

    #167934

    Purpose

    All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYC01

    Plasmid

    #224586

    Purpose

    IPTG-inducible phage φX174 lysis gene E

    Depositor

    Ophelia Venturelli

    Article

    Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

    Insert

    phage φX174 lysis gene E (phiX174p08 )
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PA1lacO-1

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHelper_TetR/pTet-Tnpase (camR)

    Plasmid

    #213910

    Purpose

    ATc-inducible himar1C9 transposase expression - cam resistance

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    TetR/ptet ATc-inducible himar1C9 transposase
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-AncMEcoGII291

    Plasmid

    #205024

    Purpose

    Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 291
    

    Tags

    His6x tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-AncMEcoGII250

    Plasmid

    #205025

    Purpose

    Expressing engineered M.EcoGII with higher thermostability

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 250
    

    Tags

    His6x tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 Dendra2

    Plasmid

    #103967

    Purpose

    Mammalian expression of fluorescent protein Dendra2 used for transfection efficiency control

    Depositor

    Qing Deng

    Article

    Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

    Insert

    Dendra2
    

    Expression

    Mammalian

    Available Since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

    Plasmid

    #41863

    Purpose

    This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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