We narrowed to 58,602 results for: plasmid
-
Plasmid#120799PurposeE. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)DepositorInsertPxyl::mCherryOpt
ExpressionBacterialMutation1.4 kb xylR-Pxyl DNA fragment from C. difficile R…PromoterPxylAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTP434
Plasmid#104167PurposeBacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labelingDepositorInsertAnti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
Tags14x Histidine tag and NEDD8 from Brachypodium dis…ExpressionBacterialMutation3x Cysteines located at bps 493-495, 520-522, and…Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
SIVu
Plasmid#81089PurposeSingle Integration Vector URA - Cloning vector for single integration into the URA3 locus of S.cerevisiae containing two MCS.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1
Plasmid#197061PurposeDonor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)DepositorInsertsEncapsulin
Adaptor Protein
UseCRISPRTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PE8max
Plasmid#257701Purposein vitro transcription plasmid for PE8maxDepositorInsertPE8max prime editor
UseTemplate for in vitro transcriptionAvailable SinceJune 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) (AAV PHP.eB)
Viral Prep#199776-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) (#199776). In addition to the viral particles, you will also receive purified AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) plasmid DNA. Cre recombinase expression in striatal direct pathway medium spiny neurons (D1-MSNs). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKIIa-jGCaMP8f-WPRE (AAV5)
Viral Prep#176750-AAV5PurposeReady-to-use AAV5 particles produced from AAV-CamKIIa-jGCaMP8f-WPRE (#176750). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8f-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCamKIIalphaAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-JRGECO1a (AAV1)
Viral Prep#128317-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-JRGECO1a (#128317). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-JRGECO1a plasmid DNA. EF1a-driven, Flp-dependent expression of the biosensor jRGECO1a. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKIIa-jGCaMP8s-WPRE (AAV1)
Viral Prep#176752-AAV1PurposeReady-to-use AAV1 particles produced from AAV-CamKIIa-jGCaMP8s-WPRE (#176752). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8s-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCamKIIalphaAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-FnCas12a-TEV
Plasmid#174658PurposeFnCas12a expression plasmidDepositorInsertFnCas12a
UseCRISPRExpressionBacterialAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-GFP-fGFP (AAV1)
Viral Prep#135631-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-S5E2-GFP-fGFP (#135631). In addition to the viral particles, you will also receive purified pAAV-S5E2-GFP-fGFP plasmid DNA. Bicistronic expression of GFP and membrane-targeted GFP under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.DepositorTagsEGFPAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
eforRed chromoprotein
Plasmid#117837PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses eforRed chromoprotein in E. coliDepositorInsertpromoter, RBS, eforRed
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1-BFP
Plasmid#120577PurposeLentiviral plasmid to co-express a guide RNA and EBFP.DepositorTypeEmpty backboneUseLentiviralAvailable SinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)
Plasmid#114070PurposeT7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tagDepositorInsertbacteria codon optimized LbCas12a with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHA2
Plasmid#85710PurposeThis plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomesDepositorTypeEmpty backboneUseCre/Lox; Herpesvirus cloningExpressionBacterialAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NADP-Snifit-cyto
Plasmid#113917PurposePlasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cellsDepositorInsertNADP-Snifit
ExpressionMammalianPromoterCMV TetO2Available SinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
loxP-myctagBFP-STOP-loxP-mCherry-NTR
Plasmid#108456PurposeThis plasmid can be used to recombine the loxP-myctagBFP-STOP-loxP-mCherry-NTR cassette (or only mCherry-NTR) into a BACDepositorInsertloxP-myctagBFP-STOP-loxP-mCherry-NTR
UseCre/LoxAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM491-Flag-His
Plasmid#109282PurposeORBIT integrating plasmid for C-terminal tagging with FLAG-His tagDepositorInsertattB-Flag-His
UseOrbit integrating plasmidAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol2-UAS-zArchon1-KGC-EGFP-ER2
Plasmid#108427PurposeCodon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmidDepositorInsertzArchon1-KGC-EGFP-ER2
UseZebrafishPromoterUASAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pgRNAtet-lvaA
Plasmid#106397PurposeGuide RNA plasmid targeting lvaA on BBR1-UP originDepositorInsertsgRNA towards lvaA in Pseudomonas putida
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only