167,433 results

1. pGFP-LacI

   Plasmid

   #179510

   Purpose

   Expresses EGFP-LacI in mammalian cells

   Depositor

   Yasushi Hiraoka

   Article

   Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

   Insert

   LacI
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PB-Ostir-neo

   Plasmid

   #161973

   Purpose

   Expresses Ostir and G418 resistance gene in mammalian cells

   Depositor

   Joanna Wysocka

   Article

   Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

   Insert

   Ostir-3xHA-p2a-neoR
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. shCD44-2 pRRL

   Plasmid

   #19123

   Depositor

   Bob Weinberg

   Article

   Godar et al Cell. 2008 Jul 11. 134(1):62-73.

   Insert

   small hairpin RNA against CD44 (CD44 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEF Synaptophysin-mRuby

   Plasmid

   #188980

   Purpose

   Synaptic buton marker

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Synaptophysin (Syp Rat, Synthetic)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNK5785

   Plasmid

   #219753

   Purpose

   pGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pGAP - nnLuz_v4 - tAOX
   

   Use

   Luciferase

   Expression

   Yeast

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSW002-PpsbA_mTurquoise2

   Plasmid

   #108237

   Purpose

   Broad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   PsbA promoter - mTurquoise2
   

   Expression

   Bacterial

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1 CARD8-TEV-GFP-FLAG

   Plasmid

   #169991

   Purpose

   Mammalian purification construct for CARD8

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Tags

   TEV-GFP-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CAG-H2BmGreenLantern

   Plasmid

   #177332

   Purpose

   To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

   Depositor

   Murray Blackmore, Gregory Petsko, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   H2B (Hist1h2bq Rat)
   

   Use

   AAV

   Tags

   mGreenLantern

   Expression

   Mammalian

   Mutation

   This fusion protein was optimized to the Human co…

   Promoter

   CAG

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PM-RA

   Plasmid

   #209878

   Purpose

   Dimerization dependent fluorescent protein RA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddRFP A
   

   Tags

   Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUCmini-iCAP-PHP.S

    Plasmid

    #103006

    Purpose

    non-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    Synthetic construct isolate AAV-PHP.S VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAGGS puro HA-hUSP27X

    Plasmid

    #225718

    Purpose

    Bacterial expression of USP27X with HA tag

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X (USP27X Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP

    Plasmid

    #213119

    Purpose

    Expression of an ActA FKBP payload for MinDE recruitment circuits in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    ActA(1-584)-BFP-FKBP (actA Listeria monocytogenes)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hCas9-VPR

    Plasmid

    #68497

    Purpose

    nuclease competent SP-Cas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    SP-Cas9-VPR
    

    Tags

    VPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Bassik lab Human CRISPR-Cas9 Deletion Library - Membrane proteins

    Pooled Library

    #101929

    Purpose

    Bassik lab Human CRISPR-Cas9 Deletion Library - Membrane proteins. Coexpresses mCherry.

    Depositor

    Michael Bassik

    Article

    Morgens et al Nat Commun. 2017 May 5;8:15178. doi: 10.

    Expression

    Mammalian

    Use

    CRISPR

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pSPneoHHgRNAH

    Plasmid

    #63557

    Purpose

    Expresses gRNA in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

    Insert

    guide sequence
    

    Use

    Leishmania expression

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-C1-FLAG-BRAT1

    Plasmid

    #161920

    Purpose

    Expresses human BRAT1 with a N-terminal GFP-FLAG tag. Confers G418 resistance.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    BRCA1-associated ATM activator 1 (BRAT1 Human)
    

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSFFV-dNme2Cas9

    Plasmid

    #193190

    Purpose

    Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    dNme2Cas9
    

    Tags

    NLS and NLS-3xHA-NLS

    Expression

    Mammalian

    Mutation

    H588A, D16A

    Promoter

    SFFV

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR5'-attP (pCM254)

    Plasmid

    #68311

    Purpose

    Gateway middle entry vector for full-length attP site for phiC31-mediated transgenesis.

    Depositor

    Christian Mosimann, Leonard Zon

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Insert

    attP
    

    Use

    Gateway entry vector

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCS2+Paxillin-mKate

    Plasmid

    #105974

    Purpose

    in vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesions

    Depositor

    Caren Norden

    Article

    Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

    Insert

    Paxillin
    

    Tags

    mKate

    Expression

    Bacterial

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIRES_IBB-mCherry-IRES-HA-mAID-nanobody

    Plasmid

    #117720

    Purpose

    Expression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNA

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Inserts

    mAID-nanobody
    IBB-mCherry
    

    Tags

    importin-beta bining domain (IBB) and triple HA

    Expression

    Mammalian

    Mutation

    codon optimized mAID for murine expression

    Promoter

    CMV and None, IRES-driven

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge