We narrowed to 8,174 results for: gnal

1. pcDNA3-Cerulean3-Cerulean3-FLARE-CKAR

   Plasmid

   #123346

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Cerulean3-Cerulean3-FLARE-CKAR
   

   Use

   Tags

   6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-PM-InPAkt

   Plasmid

   #181915

   Purpose

   Indicator of Phosphoinositides using Akt; targeted to the plasma membrane.

   Depositor

   Jin Zhang

   Article

   Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

   Insert

   pm-InPAkt
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Contains the following mutations with respect to …

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBbi-Pur-mCerulean3-cpVenus[E172]

   Plasmid

   #125203

   Purpose

   SB-transposon plasmid for stable expression of mCerulean3-cpVenus[E172] FRET high control

   Depositor

   Eric Davis

   Article

   Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

   Insert

   mCerulean3-cpVenus[E172]
   

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5 Fyn SH2:R176E

   Plasmid

   #16035

   Depositor

   Filippo Giancotti

   Article

   Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

   Insert

   c-Fyn (FYN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SH2 point mutant: R176E

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

5. GST-Rab8a-QL

   Plasmid

   #86079

   Purpose

   A bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed glutamine 67 to leucine

   Promoter

   tac

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRC/CMV-TYK2P1104A-VSV

   Plasmid

   #139350

   Purpose

   Expression of the TYK2-P1104A variant, rs34536443

   Depositor

   Sandra Pellegrini

   Article

   Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

   Insert

   TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   V362F-P1104A

   Promoter

   CMV

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEos3-caRac1

   Plasmid

   #92041

   Purpose

   Expresses mEos3 fusion with caRac1 (Q61L)

   Depositor

   Ji Yu

   Article

   Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

   Insert

   RAC1 (RAC1 Human)
   

   Use

   Tags

   mEos3

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Q61L (constitutively active)

   Promoter

   CMV

   Available since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRS6E1b-luc(-732/-721) mutant 5

   Plasmid

   #45388

   Depositor

   Thomas Gelehrter

   Article

   Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

   Insert

   6 copies of hPAI-1 promoter -732/-721 mutated at -725/723
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   -725/-723 mutated from gtt to ccc

   Promoter

   Available since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

9. AAV-FLEX-tmeGFP-IRES-nls-mRuby2

   Plasmid

   #63060

   Purpose

   Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

   Insert

   nls-mRuby2
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABEpuro-Flag-YAP2

    Plasmid

    #27472

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only

11. Cry2 mCherry PGK-1 in pcDNA3.1

    Plasmid

    #64214

    Purpose

    mCherry fused between Cry2 and PGK-1 to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    PGK1 (PGK1 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

12. MIS18A_pLENTI-CAG-IRES-GFP

    Plasmid

    #176996

    Purpose

    Mammalian lentiviral expression vector encoding MIS18A

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    MIS18A (MIS18A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. dmBACCS2VL-IRES-dOrai-IRES-mCherry

    Plasmid

    #72898

    Purpose

    Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2VL-IRES-dOrai-IRES-mCherry
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-GFP-GFP-FLARE-AKAR

    Plasmid

    #123332

    Purpose

    Green-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    GFP-GFP-FLARE-AKAR
    

    Use

    Tags

    6xHIS, EGFP, T7 tag (gene 10 leader), and Xpress …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

    Plasmid

    #173866

    Purpose

    Mammalian expression of mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3_AVPR2-NTEV-TCS-GV-2xHA

    Plasmid

    #194370

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    AVPR2-NTEV-TCS-GV-2xHA (AVPR2 Synthetic, Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. dmBACCS2NS-IRES-dOrai-IRES-mCherry

    Plasmid

    #72896

    Purpose

    Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2NS-IRES-dOrai-IRES-mCherry
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. 200 bp Axin2 mutant

    Plasmid

    #25704

    Depositor

    Eric Fearon

    Article

    Leung et al J Biol Chem. 2002 Jun 14. 277(24):21657-

    Insert

    Axin 2 promoter (AXIN2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    TCF DNA binding site at bp -108 to -102 mutated …

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pSOMA-T679A-NES

    Plasmid

    #118940

    Purpose

    Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. ELF2_pet28a

    Plasmid

    #131662

    Purpose

    Bacterial expression of ETS gene ELF2

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ELF2 (ELF2 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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