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pCSN074

Plasmid

#166731

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-NLS

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-eYFP-FKBP-MAD1-AA

Plasmid

#114038

Purpose

Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

Depositor

Geert Kops

Article

Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

Insert

Human MAD1 (MAD1L1 Human)

Tags

eYFP-FKBP12

Expression

Mammalian

Mutation

MAD2 binding deficient MAD1 K541A/L543A

Promoter

CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSTC1

Plasmid

#39241

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ11

Plasmid

#39244

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ11 system

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ12

Plasmid

#39246

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ12 system

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ12m

Plasmid

#39247

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ12 system (no transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ21

Plasmid

#39249

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ21 system

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ21m

Plasmid

#39250

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ21 system (no transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pME-secAnxaV-NS

Plasmid

#67718

Purpose

Gateway entry clone containing secreted human AnxaV with no stop codon

Depositor

Rob Parton

Article

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

secAnxaV-NS

Expression

Mammalian

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pME-miniTurbo

Plasmid

#166567

Purpose

Multisite Gateway middle entry clone encoding miniTurbo. Parton lab clone JRQ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

miniTurbo

Use

Gateway Cloning

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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acta1-mRuby2-dGBP

Plasmid

#163855

Purpose

Zebrafish muscle specific expression of mRuby2-dVHH[GFP]

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

dVHH[GFP]

Use

In vitro transcription of rna

Tags

Ruby2

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-roxSTOProx-LynEGFP

Plasmid

#133923

Purpose

Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Dre-based recombination

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

LynEGFP

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMVd1-EGFP-hRhoA-wt

Plasmid

#133926

Purpose

Low level expression of GFP fused human RhoA wild type

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

GFP-hRhoA WT (RHOA Human)

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rac1

Plasmid

#109529

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-DysF

Plasmid

#109492

Purpose

Muscle specific zebrafish expression of mKate2 fused to dysferlin

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

DysF (dysf Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pR2Q024y

Plasmid

#101022

Purpose

fluorescent, functional, glutamate receptor subunit

Depositor

Thomas Hughes

Article

Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

Insert

Glutamate receptor subunit (Gria2 Rat)

Tags

fluorescent protein

Expression

Mammalian

Mutation

tagged with fluorescent protein

Promoter

cmV

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD1 (OTU, aa 287-437)

Plasmid

#61407

Purpose

Expresses human OTUD1 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD1 (OTUD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-286 and aa 438-481.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUB2 (full-length, aa 1-234)

Plasmid

#61421

Purpose

Expresses human OTUB2 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUB2 (Ubiquitin thioesterase OTUB2) (OTUB2 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-OSBP

Plasmid

#67664

Purpose

Mammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-OSBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

Plasmid

#162335

Purpose

Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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