We narrowed to 22,217 results for: tes

1. ALKBH5-dCas9

   Plasmid

   #134783

   Purpose

   Expression fusion protein ALKBH5-dCas9 in Mammalian cells

   Depositor

   Shu-Bing Qian

   Article

   Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

   Insert

   ALKBH5 and inactive dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. FTO-dCas9

   Plasmid

   #134781

   Purpose

   Expression fusion protein FTO-dCas9 in Mammalian cells

   Depositor

   Shu-Bing Qian

   Article

   Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

   Insert

   FTO and inactive dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. px459V2.0_Conc2

   Plasmid

   #134451

   Purpose

   Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGFP-Rab8A[Q67L]

   Plasmid

   #24900

   Depositor

   Maxence Nachury

   Article

   Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

   Insert

   hRab8A[Q67L]
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Glutamine 67 to Leucine

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGFP-Rab8A[T22N]

   Plasmid

   #24899

   Depositor

   Maxence Nachury

   Article

   Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

   Insert

   hRab8A[T22N]
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Threonine 22 to Asparagine

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLG010 (DRT type 2)

   Plasmid

   #157888

   Purpose

   Expresses the DRT type 2 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   DRT type 2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBabe-c-myc-zeo

   Plasmid

   #17758

   Depositor

   Susan Lindquist

   Article

   Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

   Insert

   c-myc (MYC Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTNS2

   Plasmid

   #87802

   Purpose

   For constructing NusA-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.

   Depositor

   Yu-Zhu Zhang

   Article

   Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

   Type

   Empty backbone

   Use

   Tags

   6xHis and Strep II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRDA_478

   Plasmid

   #179096

   Purpose

   BE Cas expression

   Depositor

   John Doench, David Root

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   BE3.9 (SpG)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3.1_3XFlag

    Plasmid

    #182494

    Purpose

    Empty plasmid containing Flag tag for C-terminal tagging

    Depositor

    Paolo Comoglio

    Article

    Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

    Insert

    3xFlag with 5 Glycolic spacer
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xAP1pGL3 (3xAP-1 in pGL3-basic)

    Plasmid

    #40342

    Depositor

    Alexander Dent

    Article

    Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

    Insert

    3xAP-1
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains three canonical AP-1 binding sites (TGAC…

    Promoter

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. plenti-CAG -IRES-GFP

    Plasmid

    #69047

    Purpose

    Lentiviral backbone for GFP coexpression

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFP-Talin1

    Plasmid

    #26724

    Depositor

    Anna Huttenlocher

    Article

    Franco et al Nat Cell Biol. 2004 Oct . 6(10):977-83.

    Insert

    Talin (Tln1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mRFP-Rab5

    Plasmid

    #14437

    Purpose

    Mammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.

    Depositor

    Ari Helenius

    Article

    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

    Insert

    Rab 5 (RAB5A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pV1093

    Plasmid

    #111428

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-puro

    Plasmid

    #39481

    Purpose

    Tetracycline inducible 3rd generation lentiviral vector

    Depositor

    Ie-Ming Shih

    Article

    Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5 and 6XHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-SENP3

    Plasmid

    #34554

    Depositor

    Mary Dasso

    Article

    Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

    Insert

    SENP3 (SENP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FUGW-H1-Scrambled control shRNA

    Plasmid

    #40625

    Depositor

    Sally Temple

    Article

    Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

    Insert

    Scrambled control shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CROPseq-3P5P

    Plasmid

    #219680

    Purpose

    CROPseq vector enabling gRNA detection via mRNA-based capture (CROPseq), 3'-based direct capture using 10x capture sequence 1 or 5'-based enrichment using a custom reverse transcription primer

    Depositor

    Christoph Bock

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lact-C2-GFP

    Plasmid

    #22852

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

    Insert

    Lactadherin-C2 Domain (MFGE8 Bovine)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge