We narrowed to 9,356 results for: Mela
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pGEX-2T-CBP(1603-2678)
Plasmid#183774PurposeExpress GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.DepositorAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-FLAG-CBPΔN
Plasmid#182840PurposeA 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.DepositorInsertCBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)
UseTagsFLAG tagExpressionInsectMutationPromoterMTAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-DSep1-msfGFP
Plasmid#174494Purposebacterial expression of Drosophila Sep1 fused to monomeric superfolder GFPDepositorAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-MCS2-EF1-Puro-RGS7-R44C+V56C
Plasmid#113730PurposeExpress double mutant RGS7- R44C+V65C in mamalian cellsDepositorInsertRGS7 (RGS7 Human)
UseLentiviralTagsFlagExpressionMammalianMutationR44C and V56CPromoterCMVAvailable SinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
KHBD00021
Plasmid#34183DepositorAvailable SinceJan. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE
Plasmid#159630PurposeAAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoterDepositorInsertAst (AstA Fly)
UseAAVTagsHA, IRES, mCherry, and neurophysin-1ExpressionMammalianMutationPromoterDCA (same as CAG promoter)Available SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mScarlet-PuroR [M1G]
Plasmid#171809PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]DepositorInsertmScarlet-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-SpCas9D10A nickase
Plasmid#216737PurposeExpresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)DepositorArticleInsertCas9-D10A nickase
UseAAV and CRISPRTagsExpressionMammalianMutationD10APromoterCMVd1Available SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mScarlet-BlastR [M1G]
Plasmid#171814PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]DepositorInsertmScarlet-T2A-BlastR
UseGene taggingTagsExpressionMutationBlastR: Changed Methionin 1 to Glycine.PromoterAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3Basic-ME.2/ApoEpromoter
Plasmid#51436PurposeThis plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancerDepositorUseLuciferaseTagsExpressionMammalianMutationThe ME.2 enhancer is fused upstream of the ApoE g…PromoterAvailable SinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Phi3-AURKB-PGK-mCherry
Plasmid#153316PurposeExpresses AURKB and mCherry in mammalian cells.DepositorAvailable SinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only
