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AAV-EF1a-BbChT

Plasmid

#45186

Depositor

Dawen Cai, Joshua Sanes

Article

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Has Service

AAV9

Insert

pAAV-Ef1a-Brainbow/mCherry/mTFP-WPRE

Use

AAV; Adeno-associated viral

Expression

Mammalian

Promoter

human EF1a

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-BbTagBY

Plasmid

#45185

Depositor

Dawen Cai, Joshua Sanes

Article

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Has Service

AAV9

Insert

pAAV-EF1a-Brainbow-invert tagBFP-EYFP-WPRE

Use

AAV; Adeno-associated viral

Expression

Mammalian

Promoter

human EF1a

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Dendra2

Plasmid

#103967

Purpose

Mammalian expression of fluorescent protein Dendra2 used for transfection efficiency control

Depositor

Qing Deng

Article

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Insert

Dendra2

Expression

Mammalian

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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RCASBP-AP (A) (CC#37)

Plasmid

#15160

Depositor

Connie Cepko

Article

Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

Insert

PLAP (ALPP Human)

Use

Retroviral; Avian expression

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

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PcDNA3.1-Flag

Plasmid

#210342

Purpose

Empty Vector for molecular cloning

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

Flag tag

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pBAMD1-4

Plasmid

#61565

Purpose

Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available Since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

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NeonGreen-C1-ORP3

Plasmid

#220070

Purpose

overexpresses ORP3 with a neonGreen Fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL3 (OSBPL3 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 APEX2-NES

Plasmid

#49386

Purpose

Expresses APEX2 in the cytosol of mammalian cells

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

Flag - APEX2 - NES

Tags

Flag

Expression

Mammalian

Mutation

K14D, W41F, E112K, A134P on soybean APX; improved…

Promoter

CMV

Available Since

Nov. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pCFD5

Plasmid

#73914

Purpose

multiplex gRNA plasmid

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

dU6-3:tRNA:gRNA:tRNA:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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HyPer7.2DAAO-mito

Plasmid

#168304

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the mitochondria

Depositor

Thomas Michel

Article

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-mito

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-Mito4x-GCaMP6f

Plasmid

#127870

Purpose

Fluorescent reporter for mitochondrial matrix Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

4 repeats of COX8 signal peptide plus GCaMP6f

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ABE8e-SpRY

Plasmid

#226858

Purpose

Expresses ABE8e-SpRY base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

ABE8e-SpRY

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2

Plasmid

#81041

Purpose

Mammalian or insect expression for wt LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV, p10

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAX2

Plasmid

#117398

Purpose

allelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switch

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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HyPer7.2DAAO-NLS

Plasmid

#168302

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the nucleus

Depositor

Thomas Michel

Article

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti Vp64-dCas9 T2A mTagBFP2

Plasmid

#171052

Purpose

Expression of Vp64-dCas9 activation system

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

Vp64-dCas9 T2A mTagBFP2

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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NeonGreen-C1-ORP5

Plasmid

#220071

Purpose

overexpresses ORP5 with a neonGreen fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL5 (OSBPL5 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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Pink Flamindo

Plasmid

#102356

Purpose

biosensor for cAMP in mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Harada et al Sci Rep. 2017 Aug 4;7(1):7351. doi: 10.1

Insert

Pink Flamindo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-Zdk1

Plasmid

#81057

Purpose

mammalian expression of Zdk1

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, and mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pSOUP

Plasmid

#165419

Purpose

The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

Insert

Rep A

Use

Helper plasmid required for replication for all p…

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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