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pLenti CMV rtTA3 Blast (w756-1)
Plasmid#26429Purpose3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, BlastDepositorInsertTetracycline repressor A3 mutant
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNEU-hMbPylRS-4xU6M15
Plasmid#105830PurposePlasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cellsDepositorInsertsHumanized MbPylRS
tRNAM15
UseTagsExpressionMammalianMutation(Y271A, Y349F)MbPylRSPromoterCMV and U6Available sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-hAtg2A
Plasmid#36456DepositorInsertautophagy-related protein 2 homolog A (ATG2A Human)
UseTagsEGFPExpressionMammalianMutationchanged Proline 656 to ArgininePromoterCMVAvailable sinceMay 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCS2FA-H2B-mScarlet3
Plasmid#224436PurposeConstruct for labelling cell nuclei in mammalian cells using Histon protein 2B fused to mScarlet3DepositorInsertH2B-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMV & SP6Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMitoTimer
Plasmid#52659PurposeExpresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivoDepositorInsertDsRed1-E5
UseDsredTagshuman cytochrome c oxidase subunit VIII mitochond…ExpressionMutationPromoterCMVAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcs2-ATF4uORF1-2-GFP-IRES-mCherry
Plasmid#226105PurposeATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)DepositorInsertatf4 (ATF4 Human)
UseTagsGFPExpressionMammalianMutationcontains uORF1-2 of ATF4, whose stability can ser…PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-p15
Plasmid#62656PurposeArabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmidDepositorInsertexo, beta, gam, sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hNurr1-NE
Plasmid#102363PurposeOverexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cellsDepositorInsertNR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
UseTagsNEExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-dTAG-Puro (BRD4)
Plasmid#91796PurposePITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.DepositorInsertFKBP_F36V-2xHA-P2A-Puro
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only
