We narrowed to 5,055 results for: cre encoding plasmid

1. pEXSISERS_FLuc_loxP-PuroR

   Plasmid

   #177866

   Purpose

   Cloning Backbone for Firefly-luciferase-based EXSISERS containing loxP-sites flanked PuroR cassette

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

   Insert

   FLuc-based EXSISERS
   

   Use

   CRISPR, Cre/Lox, Luciferase, Synthetic Biology, a…

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1α1.1-FLEX-rc [Archon1-KGC-GFP-ER2]

   Plasmid

   #115891

   Purpose

   AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon1-KGC-GFP-ER2
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α promoter (1.1kb short version)

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. DRH031_ AAV-SIO-nEF-WT-THR

   Plasmid

   #225090

   Purpose

   Cre-dependent expression of wild-type thyroid receptor beta (THRB, human) in neurons driven by nEF promoter.

   Depositor

   Bernardo Sabatini

   Article

   Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

   Insert

   DN-THRB (THRB Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. DRH032_ AAV-SIO-nEF-DN-THR

   Plasmid

   #225091

   Purpose

   Cre-dependent expression of dominant-negative thyroid receptor beta (THRB, human) in neurons driven by nEF promoter.

   Depositor

   Bernardo Sabatini

   Article

   Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

   Insert

   WT-THRB (THRB Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. MADR pDonor smFP-FLAG (dark) WPRE

   Plasmid

   #131005

   Purpose

   MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-FLAG, a cytoplasmic fluorescent protein which includes multiple FLAG epitope tags

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   smFP-FLAG WPRE
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   FLAG - 10 total FLAG tags

   Expression

   Mutation

   "dark" variant with GGG fluorphore comp…

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TAC-ERP2-(N-HA)KRAS_G12D

   Plasmid

   #201254

   Purpose

   Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

   Depositor

   Alexander Kleger

   Article

   Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

   Insert

   KRAS (KRAS Human)
   

   Use

   Piggybac transposon

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   changed Gly (G) 12 to Asp (D)

   Promoter

   TRE

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEMS1052

   Plasmid

   #29004

   Purpose

   Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

   Depositor

   Elizabeth Simpson

   Article

   Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

   Insert

   Ple112 (HCRT Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Cre-EGFP-NLS

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEMS1051

   Plasmid

   #29003

   Purpose

   Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

   Depositor

   Elizabeth Simpson

   Article

   Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

   Insert

   Ple111 (HCRT Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Cre-EGFP-NLS

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-DIO-BACE-HA

   Plasmid

   #232353

   Purpose

   Beta-Secretase to be expressed (1:1) with either ATLAS protein.

   Depositor

   Don Arnold

   Article

   Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

   Insert

   BACE (BACE1 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-mGFAP(ABC1D)-2pabPAC

    Plasmid

    #234547

    Purpose

    to express the optogenetic tool 2pabPAC, which increases cAMP levels when stimulated by blue light, in astrocytes

    Depositor

    Hajime Hirase

    Article

    Vittani et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

    Insert

    2pabPAC
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    mGFAP(ABC1D)

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-mGFAP(ABC1D)-2pabPAC(F198Y)

    Plasmid

    #234548

    Purpose

    to express a version with reduced constitutive activity (thanks to point mutation F198Y) of the optogenetic tool 2pabPAC, which increases cAMP levels when stimulated by blue light, in astrocytes

    Depositor

    Hajime Hirase

    Article

    Vittani et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

    Insert

    2pabPAC(F198Y)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    F198Y

    Promoter

    mGFAP(ABC1D)

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMx-puro-MGT

    Plasmid

    #111809

    Purpose

    Retroviral expression vector for direct cardiac reprogramming

    Depositor

    Li Qian

    Article

    Wang et al Circ Res. 2015 Jan 16;116(2):237-44. doi

    Insert

    Mef2c, Tbx5 and Gata4 (Gata4, Mef2c, Tbx5 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    viral LTR

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV9:cTNT::3Flag-hYAP S127A

    Plasmid

    #86558

    Purpose

    To produce cardiac specific adeno-associated virus expressing activated human YAP.

    Depositor

    William Pu

    Article

    Lin et al Circ Res. 2014 Jul 18;115(3):354-63. doi

    Insert

    YAP (YAP1 Human)
    

    Use

    AAV

    Tags

    3Flag

    Expression

    Mutation

    Serine 127 was mutated into Alanine, to activate …

    Promoter

    cTNT

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. MADR pDonor smFP-Myc (dark) WPRE

    Plasmid

    #130987

    Purpose

    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-Myc, a cytoplasmic fluorescent protein which includes multiple Myc epitope tags

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    smFP-Myc WPRE
    

    Use

    Mosaic analysis for dual recombinase-mediated cas…

    Tags

    Myc - 10 total Myc tags

    Expression

    Mutation

    "dark" variant with GGG fluorophore ver…

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_CD3E_WT

    Plasmid

    #81865

    Purpose

    Gateway Donor vector containing CD3E , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CD3E (CD3E Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_CD3E_p.E189Q

    Plasmid

    #81314

    Purpose

    Gateway Donor vector containing CD3E , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CD3E (CD3E Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    E189Q

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-TET-MKOS

    Plasmid

    #20959

    Purpose

    piggyBac vector with tet inducible cMyc, KLF4, Oct4, Sox2 for creation of induced pluripotent stem cells

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    cMyc, KLF4, Oct4, Sox2 (Myc, Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    piggybac

    Tags

    IRES-bGeo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. FoxO6deltaCt

    Plasmid

    #124486

    Purpose

    Expresses dominant negative form of mouse FoxO6 with deleted C-term transactivation domain. T26A/S184A mutations increase nuclear localization of FoxO6.

    Depositor

    Anne Brunet

    Article

    Salih et al Genes Dev. 2012 Dec 15;26(24):2780-801.

    Insert

    FoxO6 (Foxo1, Foxo4, Foxo3, Foxo6 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T26A, S184A, transactivation domain deleted from …

    Promoter

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. AiP11818-pAAV-AiE2004m_3xC2-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN1818)

    Plasmid

    #164458

    Purpose

    AiE2004m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minCMV

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. AiP12014-pAAV-AiE2004m-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN2014)

    Plasmid

    #164457

    Purpose

    AiE2004m is an enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minCMV

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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