We narrowed to 21,236 results for: pET

1. pXWJ109As(RS-te-AAV)

   Plasmid

   #123156

   Purpose

   For TEV-based leakiness regulation. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J109, ParsR, PhrpLE

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. As2

   Plasmid

   #123160

   Purpose

   Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J109, ParsR-ABS84, PhrpLE

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXWJ109AsAmp30E

   Plasmid

   #123144

   Purpose

   Arsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J109, ParsR, PhrpLE

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXWJ109HgAmp30E

   Plasmid

   #123149

   Purpose

   Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J109, PmerT, PhrpLE

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pXW109Hg(RS-RinA)

   Plasmid

   #123150

   Purpose

   Mercury sensor with two-layered amplifier cascade. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J109, PmerT, PhrpLE, PrinA

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pXW109ABS84(RS)

   Plasmid

   #123155

   Purpose

   Amplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J109, ParsR-ABS84, PhrpLE

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. As3 (pBW411J117-arsR)

   Plasmid

   #123142

   Purpose

   An arsenic sensor with optimized intracellular receptor density. Also used in the sensor cell array. pSB3K3 carrying J117-30arsR-t-ParsR-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J117-30arsR-t-ParsR-30gfp-t
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J117, ParsR

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. As1

   Plasmid

   #123159

   Purpose

   Arsenic sensor used in the sensor cell array. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J117, ParsR, PhrpLE

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p4A8_M59I_T94M_BC

   Plasmid

   #217833

   Purpose

   4A8 variant in barcoded, yeast surface display plasmid

   Depositor

   Timothy Whitehead

   Article

   Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

   Insert

   4A8 variant Fab
   

   Expression

   Yeast

   Mutation

   VH: M59I, VL: T94M

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXW109ABS62(RS)

    Plasmid

    #123153

    Purpose

    Amplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J109, ParsR-ABS62, PhrpLE

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXW109ABS67(RS)

    Plasmid

    #123154

    Purpose

    Amplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J109. ParsR-ABS67, PhrpLE

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJMP3

    Plasmid

    #79875

    Purpose

    Bacillus subtilis sgRNA expression vector; integrates into thrC

    Depositor

    Jason Peters, Stanley Qi, Carol Gross

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    sgRNA RR1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    veg

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJMP2

    Plasmid

    #79874

    Purpose

    Bacillus subtilis sgRNA expression vector; integrates into amyE

    Depositor

    Jason Peters, Stanley Qi, Carol Gross

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    sgRNA RR1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    veg

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. As0

    Plasmid

    #123158

    Purpose

    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J109, ParsR-ABS67, PhrpLE, PrinA

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXW117As(RS-E11)

    Plasmid

    #123145

    Purpose

    Arsenic sensor with two-layered amplifier cascade. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J117, ParsR, PhrpLE, Pecf11

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJMP1

    Plasmid

    #79873

    Purpose

    Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

    Depositor

    Jason Peters, Stanley Qi, Carol Gross

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    xylA

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pXW109Hg(RS-RinA-E11)2

    Plasmid

    #123151

    Purpose

    Mercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J109, PmerT, PhrpLE, PrinA

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXW117As(RS-E11-RinA)2

    Plasmid

    #123146

    Purpose

    Arsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J117, ParsR, PhrpLE, Pecf11

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBK- Ma PylRS WT

    Plasmid

    #197573

    Purpose

    Expresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Insert

    M. alvus PylRS WT
    

    Expression

    Bacterial

    Promoter

    GlnS (constitutive)

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pALS2-sfGFP WT

    Plasmid

    #197575

    Purpose

    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replication

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP WT
    M. alvus Pyl-tRNA (6)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    araC and lpp

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAcBac1-Ma-sfGFP WT

    Plasmid

    #197567

    Purpose

    Expression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP WT
    M. alvus Pyl-tRNA (6) (4x copies)
    

    Tags

    V5-His6

    Expression

    Mammalian

    Promoter

    CMV and U6/H1

    Available Since

    April 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAcBac1-Mb-sfGFP WT

    Plasmid

    #197569

    Purpose

    Expression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP WT
    Pyl-tRNA (4 copies)
    

    Tags

    V5-His6

    Expression

    Mammalian

    Promoter

    CMV and U6/H1

    Available Since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pALS2-sfGFP 150TAG

    Plasmid

    #197574

    Purpose

    Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP 150TAG
    M. alvus Pyl-tRNA (6)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    araC and lpp

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV2-SBECN1W

    Plasmid

    #107937

    Purpose

    Adeno-Associated viral (AAV) vector plasmid expressing mouse Beclin-1 (BECN1) in neurons

    Depositor

    Johan Jakobsson

    Article

    Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

    Insert

    mouse Beclin-1 (BECN1) (Becn1 Mouse)
    

    Use

    AAV

    Promoter

    hSynapsin

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAcBac1-Ma-sfGFP 150TAG

    Plasmid

    #197568

    Purpose

    Expression of sfGFP-150TAG with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP 150TAG
    M. alvus Pyl-tRNA (6) (4x copies)
    

    Tags

    V5-His6

    Expression

    Mammalian

    Mutation

    N150TAG

    Promoter

    CMV and U6/H1

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAcBac1-Mb-sfGFP 150TAG

    Plasmid

    #197570

    Purpose

    Expression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP 150TAG
    Pyl-tRNA (4 copies)
    

    Tags

    V5-His6

    Expression

    Mammalian

    Mutation

    N150TAG

    Promoter

    CMV and U6/H1

    Available Since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAcBac1-Ma-Acridone-RS1 (A7)

    Plasmid

    #197566

    Purpose

    Expression of Methanomethylophilus alvus (Ma) Acridone RS1 (A7) tRNA synthetase/Pyl-tRNA pair for the encoding of Acridone at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    M. alvus PylRS - Acd RS1 (A7)
    M. alvus Pyl-tRNA (6) (4x copies)
    

    Tags

    None

    Expression

    Mammalian

    Mutation

    N166A, V168G, W239C

    Promoter

    CMV and U6/H1

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pNeg-Ma-barnase-TAG3-TAG45

    Plasmid

    #197572

    Purpose

    Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    Barnase - 2xTAG
    M. alvus Pyl-tRNA (6)
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    3TAG and 45TAG

    Promoter

    araC and lpp

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPos-Ma-CmR-TAG111

    Plasmid

    #197571

    Purpose

    Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    Chloramphenicol resistance gene - 111TAG
    T7 polymerase -TAG
    GFPuv
    M. alvus Pyl-tRNA(6)
    

    Expression

    Bacterial

    Mutation

    111TAG and 8TAG/112TAG

    Promoter

    AraC, T7, cat promoter (constitutive), and lpp (c…

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-hSyn-Htt171-66Q-myc-WPRE

    Plasmid

    #107934

    Purpose

    Adeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neurons

    Depositor

    Johan Jakobsson

    Article

    Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

    Insert

    Exon 1 of mutant HTT, 66 polyQ repeats (HTT Human)
    

    Use

    AAV

    Promoter

    hSynapsin

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge